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Mint polyploidy identification method

A technology for ploidy identification and mint, applied in the field of identification of mint polyploidy, can solve the problems of high technical difficulty, difficult to find the number of cells, long knocking time, etc., and achieves improved observation efficiency, short dyeing time, and dyeing. clear effect

Inactive Publication Date: 2016-05-25
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Problems solved by technology

The author has used the adventitious root tip pressing method to observe the chromosomes of Mentha plants (Observation of Chromosomes of Mentha plants, Jiangsu Agricultural Science, Issue 4, 2009, page 190), but after observing that the cells with mitotic phase obtained by this method are few, The number of cells that can be used for chromosome observation in the mid-division stage is more difficult to find, and the root tip cell structure is dense, it is difficult to disperse, and it takes a long time to knock the slices after dissociation. It is difficult to master and analyze a large number of samples.

Method used

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Embodiment 1

[0019] Example 1. Ploidy identification of two main peppermint cultivars '687' and 'Hu-39':

[0020] 1. Meristem preparation:

[0021] Select the leaves of the first and second nodes near the stem tip of the mint varieties '687' and 'Hu-39' of that year, place them in 30-fold diluted 84 disinfectant solution for 15 minutes, rinse them with running water for 30 minutes, and use them on the sterile operating table. Disinfect with 75% ethanol for 90 seconds, wash with sterile water for 3 times, place in 0.1% mercuric chloride for 10 minutes, and finally wash with sterile water for 4 times, shaking for 3-5 minutes each time. Cut into small pieces of 0.5×0.5cm long, inoculate them face up in induction medium containing 1.5mg / L 2,4-D and 1.5mg / L KT for callus induction, and the culture temperature is 20±2°C, first culture in the dark for 24h, then normal culture for 15d; under normal culture conditions, the light time is 10-16h / d, and the light intensity is 1600-2000lx; after 15d i...

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Abstract

The present invention to design a mint polyploidy identification method, an annual plant leaf is selected as an explant, callus is inducted as an observing material, and after pre-treatment, dyeing, pressing and microscope counting, polyploidy is identified. A culture and selection method and a pressing treatment method of meristem used for polyploidy observation are determined. The method is simple and effective, cells areeasy to disperse, mitosis medium-term phase cells are high in ratio and easy to find, and chromosomes dispersion is facilitated. The mint polyploidy identification method is suitable for the ploidy complex plantmint which is many in chromosome number and small in size.

Description

technical field [0001] The present invention specifically relates to a kind of mint ( Menthahap localyx Briq . ) Identification method of polyploidy. Background technique [0002] Peppermint (MenthahaplocalyxBriq.) is a perennial herb of the Lamiaceae Mentha genus. The whole herb is used as medicine to treat wind-heat cold, the beginning of wind-temperature, headache, red eyes, sore throat, sore throat, sore mouth and tongue, toothache, nettle Measles, rubella, etc., the volatile oil contained in their stems and leaves is widely used in food, medicine, cosmetics, spices, tobacco and other industries. China is recognized as the main mint producing country in the world, and Jiangsu Province is the authentic producing area of ​​mint. However, many varieties cultivated for many years have been seriously degraded, and the production of fresh grass and mint oil has declined. Polyploids have many advantages that diploids do not have, such as plant gigantism, increased resistance...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12Q1/68
CPCC12N5/04C12N5/0025C12Q1/68
Inventor 于盱梁呈元亓希武房海灵李维林
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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