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Method for producing butanol by high-temperature enzymolysis and fermentation of lignocellulose

A lignocellulose, high-temperature enzymatic hydrolysis technology, applied in the field of lignocellulose high-temperature enzymatic hydrolysis and fermentation to produce butanol, can solve the problems that are far from industrialization and the immature temperature-resistant cellulase industry, and achieve increased temperature, The effect of reducing the cost of enzymes and improving the ability to withstand high temperatures

Inactive Publication Date: 2016-06-01
LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The optimal enzymatic hydrolysis temperature of the cellulase used in the traditional cellulase process is 45-50°C. Studies have shown that enzymatic hydrolysis at a higher temperature can improve the enzymatic hydrolysis efficiency and reduce the use of cellulase. The existing high temperature resistant fiber Sulfase can be used for high-temperature enzymatic hydrolysis of sugar, and then fermented to produce butanol, but the heat-resistant cellulase industry is still immature and far from industrialized

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  • Method for producing butanol by high-temperature enzymolysis and fermentation of lignocellulose

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Embodiment 1

[0029] The lignocellulosic raw material used in the embodiment of the present invention is dry corn stalk, wherein 38.2wt% of cellulose, 22.1wt% of hemicellulose, 20.2wt% of lignin, and 3.9wt% of ash are pulverized with a pulverizer until the particle size is 1- 5mm. Dilute acid steam explosion was used for pretreatment, the reaction temperature was 190°C, the reaction time was 5min, the solid-liquid ratio was 1:2, and the concentration of dilute sulfuric acid was 2.0wt%. After coming out from the steam explosion device, adjust the pH with NaOH to be 5.0, wherein the dry matter concentration is 32wt%, the cellulose content in the dry matter is 40wt%, the xylose content in the dry matter is 20.8%, and the pretreatment dry matter recovery rate is 96wt%.

[0030] The seed liquid medium is a conventional medium for butanol fermentation strains, such as P2 and RCM, etc., and is sterilized at 115°C for 30 minutes for later use. The preparation of the seed solution is divided into t...

Embodiment 2

[0040] The method for producing butanol by high-temperature enzymatic hydrolysis and fermentation of lignocellulose of the present invention comprises the following steps:

[0041] (1) Combined pretreatment of lignocellulosic raw material corn stalks and corn stalks with dilute acid steam explosion to obtain pretreated raw materials;

[0042] (2) Add the pretreated raw materials, cellulase, water and polyethylene glycol into the enzymolysis tank for preenzymolysis, and control the dry matter concentration of the enzymolysis system to 5wt%; control the enzymolysis time to 8h; preenzyme The pH of hydrolysis is 4.5; the temperature of pre-enzymolysis is 62 ℃; the addition amount of polyethylene glycol is 0.02g / L; the addition amount of described cellulase makes the ratio of cellulase and cellulose in the pretreatment raw material be 5IU / g cellulose;

[0043] (3) After the pre-enzymolysis, the feed liquid is poured into the fermenter, and the butanol fermentation strain is added ...

Embodiment 3

[0046] The method for producing butanol by high-temperature enzymatic hydrolysis and fermentation of lignocellulose of the present invention comprises the following steps:

[0047] (1) Combined pretreatment of lignocellulosic raw material corn stalks and corn stalks with dilute acid steam explosion to obtain pretreated raw materials;

[0048] (2) Add pretreated raw materials, cellulase, water and polyethylene glycol into the enzymolysis tank for preenzymolysis, and control the dry matter concentration of the enzymolysis system to 15wt%; control the enzymolysis time to 48h; preenzyme The pH of hydrolysis is 5.5; the temperature of pre-enzymolysis is 62 ℃; the addition amount of polyethylene glycol is 0.20g / L; the addition amount of described cellulase makes the ratio of cellulase and cellulose in pretreatment raw material be 25IU / g cellulose;

[0049] (3) After the pre-enzymolysis, the feed liquid is poured into the fermenter, and the butanol fermentation strain is added to ca...

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Abstract

The invention discloses a method for producing butanol by high-temperature enzymolysis and fermentation of lignocellulose. The method comprises the following steps: (1) pretreating a lignocellulose raw material to obtain a pretreated raw material; (2) adding the pretreated raw material, cellulase, water and polyethylene glycol into an enzymolysis tank to perform pre-enzymolysis, and controlling the dry matter concentration of an enzymolysis system to be 5-15wt%; (3) adding a feed liquor after enzymolysis into a fermentation tank and adding a butanol fermentation strain to perform synchronous saccharification and fermentation; and (4) adding a fermentation liquor into a rectification device to separate butanol as well as acetone and ethanol byproducts. According to the method, polyethylene glycol is added in the enzymolysis system, so that the high-temperature tolerance of the cellulase is improved, the activity of cellulase enzymolysis under the high-temperature condition is high, the cellulase consumption is reduced, the lignocellulose enzymolysis efficiency is raised, and the economy is improved.

Description

technical field [0001] The invention belongs to the field of biomass energy, and in particular relates to a method for producing butanol by high-temperature enzymatic hydrolysis and fermentation of lignocellulose. Background technique [0002] Butanol is an important organic chemical raw material, which is widely used in chemical, pharmaceutical and petroleum industries. And because there are two more methylene groups than ethanol, butanol has higher hydrophobicity and lower volatility, can be mixed with gasoline in any proportion, and has a calorific value equivalent to gasoline. As a potential renewable bioenergy that can replace gasoline, butanol has attracted more and more attention from all over the world. [0003] With the increasing depletion of petroleum resources, reducing the proportion of fossil energy use and strengthening the development of renewable energy has become an important trend of human energy utilization in the future. Moreover, butanol produced by t...

Claims

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Application Information

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IPC IPC(8): C12P7/16C12P7/10C12P7/36C12P19/14C12R1/145
CPCC12P7/10C12P7/16C12P7/36C12P19/14Y02E50/10
Inventor 姚秀清池麟孙秀丽
Owner LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY
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