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New lactic acid bacteria, natural immune activator containing new lactic acid bacteria as active ingredients, and food and drink containing new lactic acid bacteria

A technology of natural immunity and lactic acid bacteria, applied in the field of food and beverages containing new lactic acid bacteria, can solve the problem of undiscovered CRISPR-Cas9, etc., and achieve the effect of high natural immunity activation ability and high resistance

Active Publication Date: 2019-08-06
GENOME PHARMA INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is believed that double-stranded RNA is formed in the CRISPR-Cas9 pathway, but CRISPR-Cas9 has not been found in the genome sequence of Lactococcus lactis

Method used

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  • New lactic acid bacteria, natural immune activator containing new lactic acid bacteria as active ingredients, and food and drink containing new lactic acid bacteria
  • New lactic acid bacteria, natural immune activator containing new lactic acid bacteria as active ingredients, and food and drink containing new lactic acid bacteria
  • New lactic acid bacteria, natural immune activator containing new lactic acid bacteria as active ingredients, and food and drink containing new lactic acid bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0206]

[0207] After the 11 / 19-B1 strain cultured overnight in GAM medium was sterilized at 121°C for 20 minutes, 50 μL was injected into decapitated muscle specimens of 5th instar silkworms. ) to measure innate immune activation activity.

[0208] Determination of innate immune activation activity based on slow muscle contraction according to Ishii K., Hamamoto H., Kamimura M., Sekimizu K., J. Biol. Chem. Jan. 25; 283(4): 2185-91 (2008) method described in.

[0209] That is, 0.05 mL of the above-mentioned sample was administered into the blood of a decapitated muscle specimen of a fifth-instar silkworm, and the body length was measured when the C value was maximum (about 10 minutes later), and the body length after injection was subtracted from the body length before injection. Divide this value by the body length before injection, and the obtained value is the measured C value (contraction value, Contraction Value).

Embodiment 2

[0229]

[0230] >

[0231] The cultured 11 / 19-B1 strain was sterilized at 121° C. for 20 minutes, and then concentrated. 20.0 mg of the concentrated 11 / 19-B1 strain culture solution, 40 mg of lactose, 20 mg of starch, and 5 mg of low-substituted hydroxypropylcellulose were uniformly mixed, and an 8% by mass aqueous solution of hydroxypropylmethylcellulose was used as a binder Agent, using wet granulation method to manufacture granules for tableting. 0.5 mg to 1 mg of magnesium stearate necessary for providing lubricity is added thereto, and the tablet is compressed using a tablet machine to form tablets.

[0232] >

[0233] 10.0 mg of the concentrated 11 / 19-B1 strain culture solution was dissolved in 10 mL of a 2 mass % 2-hydroxypropyl-β-cyclodextrin aqueous solution to prepare a liquid preparation for injection.

Embodiment 3

[0235]

[0236] Milk was sterilized at 95° C. for 5 minutes, then cooled to 40° C., and 0.001 parts by mass of 11 / 19-B1 strain was added. Then, it fermented at 37 degreeC for 72 hours, and obtained fermented milk. After cooling this fermented milk at 10° C. or lower, flavor and physical properties were confirmed.

[0237] As a result, both flavor and physical properties were extremely favorable.

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PUM

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Abstract

The present invention relates to a kind of lactic acid bacterium, it is the lactic acid bacterium that belongs to Lactococcus (Lactococcus) genus, and the preservation number of this lactic acid bacterium in the Patent Microbiology Deposit Center (NPMD) of Japan National Institute of Technology and Evaluation (NITE) is NITE BP-01694; A natural immune activator of the lactic acid bacteria as an active ingredient; food and drink containing the lactic acid bacteria or the natural immune activator; At least one bacterium from the group consisting of Staphylococcus aureus, Pseudomonas aeruginosa and Enterococcus monsonii is resistant.

Description

[0001] 【Technical field】 [0002] The present invention relates to a novel lactic acid bacterium, a natural immune activator containing the novel lactic acid bacterium as an active ingredient, and a food and drink containing the novel lactic acid bacterium. [0003] 【Background technique】 [0004] Lactic acid bacteria have been used to ferment food since ancient times, and are used industrially in the production of food, pharmaceuticals, and probiotics. Lactic acid bacteria are Gram-positive, catalase-negative, non-endospore-forming, and non-motile. [0005] It is known that Lactococcus lactis (L. lactis) is a non-pathogenic Gram-positive lactic acid bacterium, which does not belong to the human intestinal flora, and does not form colonies in the oral cavity and intestinal tract. In addition, its whole genome has been deciphered, and analyzes of genetics, vector system, and gene expression system have also been carried out. Recently, it has been reported that recombinant Lact...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/12A23L33/135A61K35/744A61P37/04C12R1/01
CPCA23C9/123C12N1/20A23V2002/00A61K35/744A23L33/135A61P37/04C12R2001/46C12N1/205A23V2400/231A23V2200/324A61K35/74
Inventor 关水和久西田智
Owner GENOME PHARMA INST
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