A kind of enteromorpha sea cucumber biological feed and preparation method thereof
A technology for biological feed and prolifera, which is applied in the field of prolifera sea cucumber biological feed and its preparation, can solve problems such as shortage of sea cucumber and seaweed sources, shorten the feed production time, improve the growth rate and survival rate, and improve the fermentation rate Effect
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Embodiment 1
[0023] Prepare Enteromorpha type sea cucumber biological feed raw material with enteromorpha powder: take by weighing particle size and be 200 order enteromorpha powder 40g, Ulva powder 30g, soybean meal 8g, scallop edge powder 15g, glucose 1g, shell powder 4g, vitamin premix 1g and Add 1g of mineral premix, add 300ml of distilled water, stir evenly, and adjust the pH to 9-11. Among them, each kilogram of vitamin premix contains: VA 1 200 000 IU, VD 300 000 IU, VE 5 000 mg, VB1 3 000 mg, VB2 2 000 mg, VB6 800 mg, VB12 5 mg, VC 20 000 mg, VK 33 000 mg, biotin 30 mg, inositol 10 000 mg, folic acid 300 mg, calcium pantothenate 3 000 mg, niacin 3 000 mg; per kg mineral premix contains: Cu 1 500 mg, Se 25 mg, Fe 5 000 mg, Co 500 mg, I 150 mg, Zn 2 000 mg.
Embodiment 2
[0025] Prepare enteromorpha type sea cucumber biological feed raw material with fresh enteromorpha, comprise the following steps:
[0026] ① After the fresh Enteromorpha is salvaged, wash, remove impurities, drain the surface water of the algae, and measure the water content of the fresh Enteromorpha to be 85%;
[0027] ② Take by weighing 267g of fresh Enteromorpha (equivalent to 40g dry weight of Enteromorpha), add 73ml of distilled water, and homogenate to prepare Enteromorpha homogenate;
[0028] ③ Weigh 30g of Ulva powder, 8g of soybean meal, 15g of scallop powder, 1g of glucose, 4g of shell powder, 1g of vitamin premix and 1g of mineral premix, mix well, add Enteromorpha homogenate, stir well, and adjust the pH for 9-11. Among them, each kilogram of vitamin premix contains: VA 1 200 000 IU, VD 300 000 IU, VE 5 000 mg, VB1 3 000 mg, VB2 2 000 mg, VB6 800 mg, VB12 5 mg, VC 20000 mg, VK 33 000 mg, biotin 30 mg, inositol 10 000 mg, folic acid 300 mg, calcium pantothenate 3 ...
Embodiment 3
[0030] Optimization of alkaline protease dosage:
[0031] Take alkaline protease as enzyme source to carry out enzymolysis test to embodiment 1 or 2 enteromorpha type compound feed, take total amino acid content as discriminant index to different enzyme additions to the enzymolysis effect of enteromorpha type compound feed ( figure 1 ), the results show that the total amino acid increases with the increase of the amount of alkaline protease, but the difference is not significant (P<0.05) when the amount of alkaline protease added is 8‰ and 10‰, in combination with the production cost, it is determined that the amount of alkaline protease added is 8‰ is the optimum addition amount for enzymatic hydrolysis of enteromorpha type compound feed.
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