Collection device dedicated for arterial blood sample of mouse

Abstract

The invention relates to a collection device dedicated for an arterial blood sample of a mouse. The collection device comprises a blood sample drawing device, a puncturing component and a needle stand cover; the puncturing component is arranged on the needle stand cover; the needle stand cover is connected with the blood sample drawing device; the puncturing component is a thin and soft catheter. When the collection device is used, after being subjected to general anesthesia, the mouse is fixed on a mouse board; a common carotid artery is separated and exposed; the common carotid artery is cut to form a centripetal incision forming an angle of 30 to 45 degrees relative to a blood vessel; the thin and soft catheter is inserted into the artery from the incision to a cardiac direction to draw arterial blood of required amount. As the thin and soft catheter is adopted to puncture the blood vessel, the collection device cannot penetrate through an arterial wall; the collection device is safe, simple and convenient to operate; moreover, the thin and soft catheter can be conveniently retained and fixed in the body of a laboratory animal, so that the arterial blood can be conveniently collected repeatedly.

Description

technical field [0001] The invention relates to a device specially used for collecting arterial blood samples of rats. Background technique [0002] Mice have become widely used experimental animals in biomedical research, and they are also the most well-studied mammalian experimental animals in the world today. They are often used in genetics and genetic diseases, oncology, infectious diseases, drugs, immunology, endocrine Diseases, geriatrics and other fields of research. As the global climate problem is becoming more and more serious, relevant studies have confirmed that air pollution is closely related to the incidence of respiratory diseases and cardiovascular diseases. In 2012, the international authoritative magazine JAMA confirmed that air pollution is a risk factor for cardiovascular diseases. research is also increasing. Animal models such as chronic bronchial pneumonia, acute pulmonary edema, bronchial asthma, chronic pulmonary hypertension, and pulmonary fibros...

AI Technical Summary

Problems solved by technology

Disadvantages of these three methods: 1. Methods for directly cutting off or puncturing the celiac artery: the mouse celiac artery is located behind the abdominal cavity, and the position is relatively deep. It is necessary to fully expose the mouse abdominal cavity, push the intestine, and expose the posterior abdominal wall. The abdominal aorta can only be seen after removing the retroperitoneal adipose tissue. Such a large traumatic operation will affect the respiratory function of the mouse, and then affect the accuracy of the arterial blood gas of the mouse; Contact with air will directly affect the accuracy of important blood gas indicators such as oxygen partial pressure, blood oxygen saturation, and carbon dioxide partial pressure

2. Disadvantages of collecting arterial blood by puncturing the heart of living mice with a syringe: ①The mouse heart is small, with a heart rate of 470-480 beats / min. It is difficult to collect blood from the heart, the technical requirements are high, and the mouse mortality rate is high during the operation, so it is not suitable for arterial blood Experimental research with strict blood requirements; ②It cannot be guaranteed that the collected blood is completely arterial blood, and mixed arterial and venous blood can also be collected due to the direction or operation of the needle, which will cause obvious abnormalities in arterial oxygen partial pressure and oxygen saturation

3. Chest-opening acupuncture on the right ventricle: If a death blood collection is performed by thoracotomy, once the thorax is opened, the respiratory function of the mouse is completely cut off. Accuracy of experimental data

[0004] To sum up, there are many deficiencies in the existing methods for the use of arterial blood in mice. At the same time, traditional syringes are used for the collection of arterial blood samples in mice. Due to the small size of the mouse heart and arteries, the technical requirements are high, and the mouse mortality rate is relatively high during the operation. advanced questions

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