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Preparation method of freeze-dried oil body

A technology for drying oil and oil bodies, which is applied in the field of preparation of freeze-dried oil bodies, and can solve problems such as failure to achieve results, degradation of oil body proteins, and loss of original properties of oil bodies.

Active Publication Date: 2016-06-15
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the effect is not ideal, most of the oil bodies aggregate and form large oil droplets; re-dissolution, difficult hydration, degradation of oil body protein, and loss of the original characteristics of the oil body
Especially when applied to cosmetics, the original effect cannot be achieved

Method used

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  • Preparation method of freeze-dried oil body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Purification of safflower oil bodies

[0051] Soak safflower seeds in distilled water (1:5, W / V) and place them in a refrigerator at 4-6°C for 20 hours, and then place the soaked plant seeds in a Tris-HCl buffer solution containing sodium chloride and sucrose (1 : 5, W / V, 50mmol / LTris-HCl, pH7.5, 0.4mol / L sucrose, 0.5mol / L sodium chloride), stir with a juicer mixer for 180s to obtain a plant seed homogenate. Filter the residue with a three-layer filter cloth. The filtrate was centrifuged at 4°C and 9600 rpm / min for 30 minutes to collect the milky substance on the top layer. The milky substance in the upper layer was uniformly dispersed in the above-mentioned Tris-HCl buffer solution (1:1, W / V) containing sodium chloride and sucrose, and centrifuged at 4°C and 9600rpm / min for 30 minutes to collect the milky substance in the upper layer. substance. The obtained upper layer of milky substance was uniformly dispersed in Tris-HCl buffer solution (1:5, W / V, 50mmol / LT...

Embodiment 2

[0052] Example 2 Comparison results of safflower oil body PBS emulsion (pH 8.0) added with different concentrations of mannitol to prepare oil body freeze-dried powder;

[0053] 0.20 g / ml oil body PBS emulsion (pH 8.0), with mannitol content of 0.02 g / ml, 0.04 g / ml, 0.06 g / ml, 0.08 g / ml, 0.10 g / ml, 0.12 g / ml, respectively , 0.14 g / ml, 0.16 g / ml, 0.18 g / ml, 0.20 g / ml, 0.60 g / ml, 1.00 g / ml, 1.40 g / ml, 1.80 g / ml mannitol PBS solution, the mannitol Alcoholic PBS solution includes supersaturated mannitol PBS solution. Slowly add an equal volume of co-current to the container. Stir while adding. After mixing, pre-cool at -80°C for 24 hours, then place it in a freeze dryer to dry into oil body freeze-dried powder. .

[0054] Add 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 30%, 50%, 70% to the oil body PBS emulsion containing 10% oil body , 90% mannitol, and mix the emulsion evenly and then freeze-dry to prepare dry oil body (Table 1). See the result Figure 1-31 .

[0055] It can be seen ...

Embodiment 3

[0057] Example 3 Oil body resuspension, preparation of oil body emulsion, and oil body freeze drying

[0058] 1) PBS solution: PBS solution containing (weight) 0.03-0.05% methyl paraben + 0.03-0.05% propyl paraben, pH 8.0-8.5;

[0059] 2) Oil body PBS emulsion: The purified vegetable oil body is resuspended in the above PBS solution to prepare an oil body PBS emulsion;

[0060] 3) Mannitol PBS solution: Dissolve mannitol into the above PBS solution to prepare mannitol PBS solution;

[0061] 4) Oil body mannitol PBS emulsion: Add the oil body PBS emulsion and mannitol PBS solution into the container slowly in parallel flow, and stir while adding; to obtain the oil body mannitol PBS emulsion, the final concentration of the oil body is 0.05-0.20 g / ml, the final concentration of mannitol is 0.8-3 times that of oil bodies.

[0062] 5) Pre-freeze the oil body mannitol PBS emulsion and place it in a freeze dryer to obtain a freeze-dried oil body powder.

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Abstract

The invention discloses a preparation method of freeze-dried oil body. The preparation method includes the steps of: 1) preparing an oil body PBS emulsion and a mannitol PBS solution respectively from a PBS solution containing 0.03-0.05 wt% of methylparaben and 0.03-0.05 wt% of propylparaben and being 8.0-8.5 in pH value; 2) adding the two solutions hereinabove into a container at equal volume in a parallel flow manner with stirring to obtain an oil body / mannitol PBS emulsion, wherein the final concentration of the oil body is 0.05-0.20 g / ml and the final concentration of mannitol is 0.8-3 times of that of the oil body; and 3) pre-freezing the mixture, and freeze-drying the mixture in a freeze-drying machine. The oil body freeze-dried powder, after six months at room temperature, has uniform appearance particle size, has no significant color change, and is easy to re-dissolve and hydrate. Microscope structure detection after hydration proves that the oil body is free of large oil drops, is homogenous, and has no bacterial colony after bacterial detection. The method solves a problem that the oil body is difficult to store. The oil body is convenient to store and transport, is reduced in the content of mannitol, and provides basis for utilizing the oil body to produce foods, cosmetics and medicines better.

Description

Technical field [0001] The invention belongs to the fields of food deep processing, cosmetics and medicines, and specifically is a method for preparing freeze-dried oil bodies. Background technique [0002] Oil bodies are the smallest organelles in plant cells. The organelles sometimes called oil droplets, lipid droplet particles or round spheres. The oil bodies are spheres with a diameter of 0.5-2.5 μm. The size of the oil bodies varies with plant species and varieties, and is affected by nutrition and the environment. . In different tissue cells of the same seed, the size of the oil body will be different. Under the electron microscope, the outside of the oil body is a dense film, and the inside is an opaque matrix. From a physiological point of view, if the diameter of the oil body is less than 0.2 μm, a large amount of phospholipids (PL) and oil body protein (oleosin) need to be consumed. On the contrary, if the diameter of the oil body is greater than 2.5 μm, the lipase c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/92A61K8/20A61K8/60A61K8/34A61K8/37A61K47/44A61K47/26A61K47/14
CPCA61K8/20A61K8/345A61K8/37A61K8/60A61K8/922A61K47/14A61K47/26A61K47/44
Inventor 李海燕李校堃官丽莉杨晶杜林娜王法微王艳芳郭咏昕高红桃
Owner JILIN AGRICULTURAL UNIV
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