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Preparation method of phosphorylcholine bionic coating

A phosphorylcholine and coating technology, which is applied in the field of material surface science and biomedical polymer materials, can solve the problem of difficulty in obtaining a high-content phosphorylcholine group surface, and the rigidity of the fixed group hindering the phosphorylcholine group. Orientation migration, limiting biocompatibility and other issues, to achieve the effect of improved hydrophilicity and blood compatibility, simple method and mild conditions

Inactive Publication Date: 2016-06-15
XIAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods obtain a relatively stable imitation cell outer membrane structure coating, the rigidity of the fixing group also hinders the maximum orientation migration of the phosphorylcholine group, making it difficult to obtain a high content of phosphorylcholine group clumped surface, which limits the further improvement of biocompatibility

Method used

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  • Preparation method of phosphorylcholine bionic coating
  • Preparation method of phosphorylcholine bionic coating
  • Preparation method of phosphorylcholine bionic coating

Examples

Experimental program
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Embodiment 1

[0038] This embodiment includes the following steps:

[0039] Step 1. Under nitrogen protection conditions, dissolve 16mmol of 2-methacryloyloxyethyl phosphorylcholine monomer and 4mmol of 2-aminoethyl methacrylate hydrochloride monomer in distilled water in 0.1mmol of initiator Carry out free radical polymerization under the influence of free radical polymerization. The reaction temperature of free radical polymerization is 70°C, and the reaction time is 12h. Freeze-drying under low temperature, obtain the phosphorylcholine polymer containing amino group; Described initiator is potassium persulfate;

[0040] Step 2. Dissolve 1 mg of the amino group-containing phosphorylcholine polymer described in step 1 in 1 mL of methanol to obtain a polymer solution, then add 15 μL of glutaraldehyde aqueous solution to the polymer solution, mix well, and obtain a mixed Solution; The mass concentration of described glutaraldehyde aqueous solution is 50%;

[0041] Step 3. Apply the mixed s...

Embodiment 2

[0044] Example 2 is the same as Example 1, except that the polycarbonate film obtained in step 3 with a phosphorylcholine biomimetic coating on its surface is heated for 12 hours under a vacuum condition of 110° C., and washed with distilled water Finally, the phosphorylcholine biomimetic coating after hydrophobic treatment was obtained.

Embodiment 3

[0056] This embodiment includes the following steps:

[0057] Step 1. Under the condition of nitrogen protection, 14 mmol of acryloyloxyethyl phosphorylcholine monomer and 6 mmol of 2-aminoethyl methacrylate hydrochloride monomer are dissolved in distilled water and then carried out under the action of 0.2 mmol of initiator Free radical polymerization, the reaction temperature of free radical polymerization is 60°C, and the reaction time is 20h. After the reaction, the reaction solution is concentrated, then dialyzed with a dialysis bag with a molecular weight cut-off of 6000D to 8000D, and then freeze-dried at -50°C , to obtain phosphorylcholine polymers containing amino groups; the initiator is ammonium persulfate;

[0058] Step 2. Dissolve 0.5 mg of the amino group-containing phosphorylcholine polymer in 1 mL of methanol to obtain a polymer solution, then add 10 μL of glutaraldehyde aqueous solution to the polymer solution, and mix well to obtain Mixed solution; the mass c...

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Abstract

The invention discloses a preparation method of a phosphorylcholine bionic coating. The preparation method comprises the following steps of 1, performing free radical polymerization on ethylene monomers containing phosphorylcholine groups and ethylene monomers containing amino groups under the effect of initiators to obtain phosphorylcholine polymers containing amino groups; 2, dissolving the phosphorylcholine polymers containing amino groups into a polar solvent to obtain a polymer solution; then, adding a glutaraldehyde water solution; uniformly mixing the materials to obtain a mixed solution; 3, coating the mixed solution onto the surface of a material to be modified; after drying, putting the material to be modified into distilled water; soaking the materials for 6h to 12h under the condition of the temperature being 40 DEG C to 80 DEG C; after the materials are taken out, obtaining the phosphorylcholine bionic coating on the surface of the material to be modified. The preparation method has the advantages that the method is simple; the conditions are mild; the method is hopeful to become a novel path for regulating and controlling the action of biological materials with biological activity substances such as protein and platelet; important academic significance is realized.

Description

technical field [0001] The invention belongs to the technical fields of material surface science and biomedical polymer materials, and in particular relates to a preparation method of a phosphorylcholine bionic coating. Background technique [0002] When the material is used in a living body, it is easy to non-specifically adsorb proteins, activate complement molecules and the immune system, thereby causing blood coagulation, immune and inflammatory reactions, resulting in a significant decrease in its performance or even failure. This is due to the poor biocompatibility of materials, therefore, biocompatibility research has become the primary issue in the field of biomaterial research. The surface of a material is the medium through which the material contacts the organism. Surface charge, hydrophilicity / hydrophobicity, chemical composition, and morphology are important factors that affect the interface interaction between the material and the organism, and determine whethe...

Claims

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Application Information

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IPC IPC(8): C08J7/04C08L69/00C09D143/02C09D7/12C08F230/02C08F220/34C08F220/60
CPCC09D7/63C09D143/02C08F230/02C08K5/07C08J2443/02C08J2369/00C08J7/0427C08F220/34C08F220/60
Inventor 宫铭
Owner XIAN UNIV OF SCI & TECH
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