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Superparamagnetism functional particles, magnetized red blood cells and clinic application of superparamagnetism functional particles and magnetized red blood cells

A superparamagnetic, red blood cell technology, applied in the field of medical testing, can solve the problem of no disclosure of blood group antibodies, etc., and achieve the effects of a simple serological detection test method, easy dispersion, and uniform size

Active Publication Date: 2016-06-22
BEIJING GUO KE RONG ZHI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Bacterial magnetic particles are a kind of natural magnetic nano-biological materials, which have huge potential application value in many fields due to their excellent properties. For example, the bacterial magnetic particles coated with anti-Bt insecticidal protein polyclonal antibodies can be used for detection Bt insecticidal protein in mouse tissue; CN101077418 disclosed loaded doxorubicin bacterial nano-bacteria magnetic particles can be used to inhibit the proliferation of cancer cells, etc.; but the prior art has not disclosed the use of bacterial magnetic particles and anti-erythrocyte glycoprotein GPA monoclonal antibody Conjugated and used for blood group antibody detection related reports

Method used

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  • Superparamagnetism functional particles, magnetized red blood cells and clinic application of superparamagnetism functional particles and magnetized red blood cells
  • Superparamagnetism functional particles, magnetized red blood cells and clinic application of superparamagnetism functional particles and magnetized red blood cells
  • Superparamagnetism functional particles, magnetized red blood cells and clinic application of superparamagnetism functional particles and magnetized red blood cells

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Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1 A kind of superparamagnetic functional particle

[0051] The particles are incubated with bacterial magnetic particles and anti-erythrocyte glycoprotein GPA monoclonal antibody after the surface group is activated by the coupling agent:

[0052] The preparation method of the superparamagnetic functional particle is as follows:

[0053] a. Activation: adding a mixture of EDC and NHS with a mass ratio of 1:1 to activate the bacterial magnetic particles to obtain activated bacterial magnetic particles;

[0054] b. Incubation: incubate the activated bacterial magnetic particles with anti-erythrocyte glycoprotein GPA monoclonal antibody at room temperature;

[0055] c. Separation: Magnetic adsorption and washing to obtain superparamagnetic functional particles.

Embodiment 2

[0056] Embodiment 2 A kind of superparamagnetic functional particle

[0057] The particles are incubated with bacterial magnetic particles and anti-erythrocyte glycoprotein GPA monoclonal antibody after the surface group is activated by the coupling agent:

[0058] The preparation method of the superparamagnetic functional particle is as follows:

[0059] a. Activation: adding a mixture of EDC and NHS with a mass ratio of 1:1 to activate the bacterial magnetic particles to obtain activated bacterial magnetic particles;

[0060] b. Incubation: the activated bacterial magnetic particles and the anti-erythrocyte glycoprotein GPA monoclonal antibody are added to an incubation agent and incubated at room temperature; mixture;

[0061] c. Separation: Magnetic adsorption and washing to obtain superparamagnetic functional particles.

Embodiment 3

[0062] Embodiment 3 A kind of superparamagnetic functional particles

[0063] The particles are incubated with bacterial magnetic particles and anti-erythrocyte glycoprotein GPA monoclonal antibody after the surface group is activated by the coupling agent:

[0064] The preparation method of the superparamagnetic functional particle is as follows:

[0065] (1) Take bacterial magnetic particles, add a treatment agent to react under stirring conditions, and wash the product until neutral after the reaction; mixed solution;

[0066] (2) The bacterial magnetic particles obtained after the step (1) reaction is made into a solution with a concentration of 0.1%, and the pH value of the solution is adjusted to 3.0. Adding a concentration of 5% 3-aminopropyl triethoxy to the above solution In the silane ethanol solution, react under stirring conditions, wash the product to neutrality after the reaction is completed, and prepare the treated bacterial magnetic particles;

[0067] (3) ...

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PUM

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Abstract

The invention provides superparamagnetism functional particles and magnetized red blood cells. The surfaces of bacterial magnetic particles naturally carry rich -NH2 groups, the bacterial magnetic particles are modified through a certain chemical method so as to be activated and are in coupling connection with an anti-red blood cell GP (Glycoprotein) A monoclonal antibody, so that the superparamagnetism functional particles are prepared; before clinical blood group antibody detection, the superparamagnetism functional particles can be in incubation with red blood cells and can be quickly magnetized, then sample detection can be carried out, and centrifugal separation is replaced with magnetic separation during a detection process. A quick, simple and convenient testing method is provided for clinical application, and the superparamagnetism functional particles provided by the invention are especially beneficial for quick magnetization of the red blood cells and solid-liquid separation during a clinical blood group antibody detection process.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to superparamagnetic functional particles, magnetized red blood cells and their clinical application. Background technique [0002] Red blood cell blood type is one of the human genetic polymorphic markers. Since the discovery of ABO blood type in the early 20th century, more than 500 human red blood cell blood group antigens have been discovered, and more than 200 have been confirmed so far. Among them, the blood group system most closely related to clinical practice includes 29 systems such as ABO, Rh, and MNS. Clinical blood group serology testing mainly includes: blood group testing, antibody screening and identification, and cross-matching. At present, the red blood cells used in blood group serological testing are all fresh red blood cells. During the detection process, the red blood cells need to react with the sample, and the results can be observed after centrifugation. Howe...

Claims

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Application Information

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IPC IPC(8): G01N33/80G01N33/577
CPCG01N33/577G01N33/80
Inventor 张金菊王红光
Owner BEIJING GUO KE RONG ZHI BIOTECH CO LTD
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