Jerusalem artichoke leaf dichloromethane extract emulsion and preparation method and application thereof
A dichloromethane and extract technology, applied in the field of Jerusalem artichoke leaf dichloromethane extract emulsion and preparation thereof, can solve problems such as blank of Jerusalem artichoke leaf emulsion, and achieve the effect of increasing concentration efficiency
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Embodiment 1
[0034] The Jerusalem artichoke variety used in the method is South Jerusalem artichoke No. 1, provided by the Marine Tidal Flat Resources Laboratory of the School of Resources and Environment, Nanjing Agricultural University, and planted in Jinhai Farm, Dafeng City, Yancheng, Jiangsu Province. The waste leaves of Jerusalem artichoke were collected from September to November and placed in 105 ℃ oven for 15 minutes to kill green, then transfer to 75 ℃, 1 ~ 2d drying, then take out and store for later use.
[0035] 1) Put Jerusalem artichoke leaves in a container and then pour 70% ethanol (w / v ratio is 1:4), extract for 25 days, stir 3 times a day, 5 minutes each time; use vacuum pumping device for ethanol extract Suction filtration, the filtrate was rotary evaporated with a rotary evaporator, and the ethanol was removed by rotary evaporation; the extract obtained by rotary evaporation was extracted with petroleum ether and dichloromethane to obtain petroleum ether extract, dichlo...
Embodiment 2
[0053] Embodiment 2 Microemulsion quality detection
[0054] The extract of Jerusalem artichoke leaves prepared in Example 1 is 5%, DBS-Na 2.5%, OP-107.5%, ethanol 12%, methanol 4%, cyclohexanone 6%, the balance is water, and the total mass of each raw material is 100%. The sub-content is 100%.
[0055] Add solvent and emulsifier combination to Jerusalem artichoke leaf dichloromethane extract according to the percentage content of Jerusalem artichoke leaf dichloromethane extract, solvent, emulsifier combination, co-solvent or co-surfactant and water one by one , co-solvent or co-surfactant and water, stir evenly, and adjust the pH of the dichloromethane extract of Jerusalem artichoke leaves to 5-8 with acid or alkali to obtain the dichloromethane extract emulsion of Jerusalem artichoke leaves.
[0056] Choice of water quality
[0057] The water quality with different hardness below 342mg / L (calculated as calcium carbonate concentration) was screened out. The results of the m...
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