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Inactivated vaccine for mink pseudomonas aeruginosa

A technology of mink Pseudomonas aeruginosa and inactivated vaccines, applied in vaccines, veterinary vaccines, antibacterial drugs, etc., can solve the problems of weak immunogenicity and low immune cross-protection, and reduce morbidity and mortality , prevent epidemics and spread, and reduce economic losses

Inactive Publication Date: 2016-07-06
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide an inactivated vaccine of Pseudomonas aeruginosa in mink, thereby solving the problems of weak immunogenicity of existing vaccine strains and low immune cross-protection between each serotype in the research and development of Pseudomonas aeruginosa inactivated vaccine in mink

Method used

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  • Inactivated vaccine for mink pseudomonas aeruginosa
  • Inactivated vaccine for mink pseudomonas aeruginosa
  • Inactivated vaccine for mink pseudomonas aeruginosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1.1 Isolation and purification method of Pseudomonas aeruginosa bacterial strain

[0019] The affected minks were selected from a large mink farm suspected of hemorrhagic pneumonia in Liaoning Province, and the lungs, liver and heart blood of the sick minks were aseptically collected and inoculated on cetyltrimethylammonium bromide agar medium (NAC) , placed in a 37°C incubator for 16h to 18h, picked round, smooth, moist, and flat colonies for Gram staining and microscopic examination, and selected Gram-negative small bacilli for streaking and pure culture.

[0020] Stratify the pure culture strains on fresh blood agar plate and NAC plate respectively, culture at 37°C for 16h~18h, choose to produce green fluorescent pigment on hexadecanetrimethylammonium bromide medium, and produce β on blood agar medium Hemolyzed small colonies were purified and cultured.

[0021] According to the biochemical identification test requirements of Pseudomonas aeruginosa, the two strains ...

Embodiment 2

[0046] Application of mink Pseudomonas aeruginosa type B LN03 strain in preparation of inactivated vaccine.

[0047] The preservation number of the strain is taken as the Pseudomonas aeruginosa type B LN03 strain with the preservation number CCTCCNO: M2016068. After activation, the culture medium is inoculated respectively, the bacterial liquid is collected after cultivation, inactivated by formaldehyde solution, and mixed with propolis adjuvant to prepare inactivated Propolis vaccine.

[0048] The preferred operation steps are as follows:

[0049] 2.1 Strain selection

[0050] The strain used for seedling production was Pseudomonas aeruginosa type B LN03 strain, which was stable in colony morphology, bacterial characteristics, biochemical characteristics, and culture characteristics, and had strong pathogenicity to minks. 1.0mL bacterial liquid (5.0×10 6 CFU / mL) injection can cause 100% of 21 to 42 days old mink disease. The immunogenicity of mink Pseudomonas aeruginosa ty...

Embodiment 3

[0091] Application of mink Pseudomonas aeruginosa type B LN03 strain in preparation of inactivated vaccine.

[0092] The preservation number of the strain is taken as Pseudomonas aeruginosa type B LN03 strain with the preservation number CCTCCNO: M2016068. After activation, the culture medium is inoculated respectively, the bacterial liquid is collected after cultivation, inactivated by formaldehyde solution, and mixed with water adjuvant to prepare inactivated vaccine.

[0093] The preferred operation steps are as follows:

[0094] 2.1 Strain selection

[0095] The strain used for seedling production was Pseudomonas aeruginosa type B LN03 strain, which was stable in colony morphology, bacterial characteristics, biochemical characteristics, and culture characteristics, and had strong pathogenicity to minks. 1.0mL bacterial liquid (5.0×10 6 CFU / mL) injection can cause 100% of 21 to 42 days old mink disease. The immunogenicity of mink Pseudomonas aeruginosa type B LN03 strain...

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Abstract

The invention provides an inactivated mink Pseudomonas aeruginosa vaccine, which includes an antigen and a vaccine adjuvant, wherein the antigen used is type B mink Pseudomonas aeruginosa LN03 strain, and the preservation number is CCTCC NO: M2016068. The inactivated vaccine prepared by the invention is safe and reliable, can provide effective homologous virus attack protection, can produce strong immunity after immunization, significantly reduces the morbidity and mortality of inoculated mink groups, and can effectively prevent the prevalence of mink hemorrhagic pneumonia It can reduce the economic loss caused by the disease to the fur animal breeding industry, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, and in particular relates to an inactivated vaccine of mink Pseudomonas aeruginosa. Background technique [0002] Mink hemorrhagic pneumonia, also known as mink Pseudomonas pneumonia, is an acute infectious disease that mainly occurs during the moulting season of mink from August to November every year. It is characterized by hemorrhagic pneumonia and acute death. Nostrils flowed out of red foamy liquid and other symptoms. Post-mortem examination showed that the entire lung lobe was enlarged with diffuse hemorrhage and sepsis. The disease occurs all over the world and can cause 20%-40% mortality in farms every year. It is an important bacterial infectious disease that endangers the fur animal breeding industry. [0003] The disease pathogen is Pseudomonas aeruginolsa (Pseudomonasaeruginolsa) in Pseudomonas (Pseudomonadaceae) Pseudomonas (Pseudomonas), also known as Pseudomonas aerugi...

Claims

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Application Information

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IPC IPC(8): A61K39/104A61K39/39A61P11/00A61P31/04A61P7/04
CPCA61K39/104A61K39/39A61K2039/521A61K2039/552A61K2039/55505A61K2039/55588
Inventor 单虎张洪亮盖春云黄娟张传美秦志华杨瑞梅秦晓冰
Owner QINGDAO AGRI UNIV
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