Method for extracting anthocyanins and ursolic acid from perilla leaves

A technology of perilla leaves and ursolic acid, applied in chemical instruments and methods, preparation of sugar derivatives, steroids, etc., can solve the problems of anthocyanin destruction, easy destruction of anthocyanins, etc., to promote rapid release and reduce production. cost, reducing the effect of overuse

Active Publication Date: 2018-03-20
广东冠龙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0023] The present invention aims at the problems such as easy destruction of anthocyanins in the process of extracting ursolic acid from perilla leaves, provides a method for extracting ursolic acid and anthocyanins from perilla leaves, and thoroughly solves the problems of anthocyanins destruction in the extraction process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049]Get 300kg of fresh perilla leaves and use a tea drum fixer to fix them for 2 to 3 minutes (the water content of perilla leaves is 75%), and immediately mince them; add 700kg of ethyl acetate or petroleum ether to extract once at room temperature, and add the residue containing 800 L of 0.005 mol / L hydrochloric acid aqueous solution of 85% ethanol was extracted at room temperature for 4 hours. The extract was filtered twice with three layers of gauze and once with a ceramic membrane to obtain filtrate I, which was then concentrated to 40 L under vacuum or reduced pressure at 45°C, and filtered once with a ceramic membrane to obtain 3.68 kg of filtrate II and filter residue I. The filtrate II was adsorbed on a NKA-9 macroporous resin column, and eluted with three column volumes of 25% ethanol and pH 3 aqueous solution, and the eluate I was collected. The eluent I was concentrated to dryness under vacuum or reduced pressure at 45°C to obtain 1.45 kg of pure anthocyanin. Ad...

Embodiment 2

[0050] Take 500kg of fresh perilla leaves and use a tea drum fixer to fix them for 2 to 3 minutes (the water content of perilla leaves is 80%), and immediately grind them; add 1200kg of ethyl acetate or petroleum ether to extract once at room temperature, and add the residue containing 85% ethanol 0.005mol / L hydrochloric acid aqueous solution 1200L, leaching at room temperature for 4h. The extract was filtered twice with three layers of gauze and once with a ceramic membrane to obtain filtrate I, then concentrated to 80 L under vacuum or reduced pressure at 45°C, and filtered once with a ceramic membrane to obtain 5.75 kg of filtrate II and filter residue I. The filtrate II was adsorbed on the NKA-9 macroporous resin column, and eluted with three column volumes of 20% ethanol and pH 3 aqueous solution, and the eluate I was collected. The eluate I was concentrated to dryness under vacuum or reduced pressure at 45°C to obtain 2.64 kg of pure anthocyanin. Add the filter residue ...

Embodiment 3

[0052] Take 800kg of fresh perilla leaves with a water content of 80% and use a tea roller degreening machine to fix them for 2 to 3 minutes, and immediately grind them; 0.008mol / L hydrochloric acid aqueous solution 2800L, leaching at room temperature for 4h. The extract was filtered twice with three layers of gauze and once with a ceramic membrane to obtain filtrate I, which was then concentrated to 150 L under vacuum or reduced pressure at 45°C, and filtered once with a ceramic membrane to obtain 9.86 kg of filtrate II and filter residue I. The filtrate II was adsorbed on a NKA-9 macroporous resin column, and eluted with three column volumes of 20% ethanol and pH 3.5 aqueous solution, and the eluate I was collected. The eluate I was concentrated to dryness under vacuum or reduced pressure at 45°C to obtain 4.56 kg of pure anthocyanin. Add the filter residue I to 20L of anhydrous methanol, put it on a 200-mesh silica gel column for adsorption, and use five times the column v...

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Abstract

The invention provides a method for extracting anthocyanin and ursolic acid from perilla leaves. The crystals of ursolic acid and anthocyanin are obtained by a method combining enzyme deactivation, petroleum ether degreasing, acid water extraction, resin and crystallization. Through the acid water extraction technology, a large amount of high-purity water-soluble anthocyanin is obtained, the anthocyanin extraction rate is effectively increased, and the risk of environmental pollution caused by insufficient utilization of anthocyanin is reduced. Through enzyme deactivation and petroleum ether degreasing, the damage of anthocyanin and ursolic acid caused by enzyme can be effectively prevented while the cells of perilla leaves are damaged and quick release of active ingredients in the leaves can be promoted.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and relates to a method for extracting ursolic acid and anthocyanin by using fresh perilla leaves. Background technique [0002] The ursolic acid contained in the plant is a triterpenoid compound, which has a wide range of uses in medicine. Can be used as: liver protection, anti-hepatitis, anti-oxidation, anti-bacterial, anti-inflammatory and anti-viral effects, blood fat-lowering, anti-atherosclerosis, blood sugar-lowering and anti-ulcer effects, HIV-inhibiting, calming And stable effect, has the effect of enhancing immune function. Because ursolic acid is an active ingredient in plants, it is widely used in medicine and medical science. It is proved that ursolic acid is a broad-spectrum antibiotic, so it was included in the 2002 "863" plan by the state. [0003] At present, although ursolic acid is relatively difficult to chemically synthesize, it is widely found in plants ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/065C07H1/08C07J63/00
CPCC07H1/08C07H17/065C07J63/008
Inventor 向华唐忠海
Owner 广东冠龙生物科技有限公司
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