Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of muc21 gene and its expression product in diagnosis and treatment of pituitary tumor

A technology for expressing products and pituitary tumors, which can be used in the field of biomedicine to solve problems such as injury, blindness, and coma.

Active Publication Date: 2019-10-11
QINGDAO MEDINTELL BIOMEDICAL CO LTD
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of pituitary tumors includes drug therapy, surgical treatment, radiotherapy and comprehensive treatment. Surgical treatment is the first choice for other types of pituitary tumors. However, traditional surgical treatment will cause a certain degree of damage while removing the tumor. Some patients may even experience severe postoperative complications such as postoperative blindness, permanent diabetes insipidus, long-term high fever, and coma due to different surgical positioning or resection degrees
Secondary radiation brain edema after radiotherapy is still an uncontrollable difficulty in current radiotherapy techniques

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of muc21 gene and its expression product in diagnosis and treatment of pituitary tumor
  • Application of muc21 gene and its expression product in diagnosis and treatment of pituitary tumor
  • Application of muc21 gene and its expression product in diagnosis and treatment of pituitary tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Screening for gene markers associated with pituitary tumors

[0062] 1. Sample collection

[0063] Six samples of normal pituitary tissue and pituitary tumor tissue were collected. All the above specimens were obtained with the consent of the organizational ethics committee.

[0064] 2. RNA sample preparation (using miRNA kit for operation)

[0065] The tissues obtained above were shredded, put into liquid nitrogen and ground into powder, and RNA was extracted and isolated according to the instructions in the kit. details as follows:

[0066] 1) Isolation of RNA:

[0067] A. Add in tissue homogenate or cells Reagent II 1ml;

[0068] B. Stand at room temperature for 3 minutes, add 0.2ml chloroform and shake vigorously for 15 seconds;

[0069] C. Place on ice for 10 minutes;

[0070] D. Centrifuge at 12000g for 15min at 4°C;

[0071] E. Transfer 80% of the aqueous phase into a new 2ml EP tube, add 1 / 2 the amount of absolute ethanol, and shake;

[00...

Embodiment 2

[0086] Example 2 QPCR sequencing to verify the differential expression of MUC21 gene

[0087] 1. Large-sample QPCR verification of differential expression of MUC21 gene. According to the sample collection method in Example 1, 50 cases of normal pituitary tissues and 50 cases of pituitary tumor tissues were selected.

[0088] 2. The specific operation steps of QPCR are as follows:

[0089] (1) RNA extraction

[0090] The RNA extraction procedure was as described in Example 1.

[0091] (2) reverse transcription

[0092] A. Configure the mixed solution in Microtube: Oligo dT (50μM) 1μl, dNTP mixed solution (10mM) 1μl, RNA template 5μg, add ddH 2 0 to 10 μl, mix well;

[0093] B. Carry out denaturation and annealing reactions on the PCR instrument according to the following reaction conditions: after 5 minutes at 65°C, place it on ice immediately;

[0094] C. Prepare the following reverse transcription reaction solution in the above-mentioned Microtube tube:

[0095] 10 μl ...

Embodiment 3

[0113] Example 3 Overexpression of MUC21 gene

[0114] 1. Cell culture

[0115] Human pituitary tumor cell line GT1.1 was incubated at 37°C and 5% CO in medium 1640 containing 10% fetal bovine serum and 1% P / S. 2 , Cultivated in an incubator with a relative humidity of 90%. Change the medium once every 2-3 days, and use 0.25% EDTA-containing trypsin for routine digestion and passage.

[0116] 2. Overexpression of MUC21 gene

[0117] 2.1 Construction of MUC21 gene expression vector

[0118] Amplification primers were designed according to the coding sequence of the MUC21 gene (as shown in SEQ ID NO.1), and the primer sequences were as follows:

[0119] Forward primer: 5'-CCGAAGCTTGCCACCATGAAGATGCAGA-3'(SEQ ID NO.7)

[0120] Reverse primer: 5'-CGGGCGGCCGCGGGCCCGCTGTTCCTCC-3' (SEQ ID NO.8)

[0121] The coding sequence of the full-length MUC21 gene was amplified from the cDNA library of adult fetal brain (clontech company, product number: 638831), and the cDNA sequence was i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a gene MUC21 and application of an expression product of the gene MUC21 in hypophysoma diagnosis and treatment.The gene MUC21 related to generation and development of hypophysoma is screened through a bioinformatics method, function verification is conducted through a fluorescent quantitative method and the like, and down-regulated expression of the gene MUC21 is conducted in hypophysoma patient tissue; by increasing the expression of the gene MUC21, the excessive proliferation of hypophysoma cells can be restrained.The invention further discloses a product for diagnosing hypophysoma and a medicine composition for treating hypophysoma.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of MUC21 gene and its expression product in the diagnosis and treatment of pituitary tumors. Background technique [0002] Pituitary tumor is a common intracranial tumor, accounting for about 10% to 20% of intracranial tumors. It is derived from the cells of the anterior pituitary gland, and is a clonal proliferation of tumor cells, showing the characteristics of no hormone or increased hormone secretion. The vast majority of pituitary tumors are benign adenomas, and very few are carcinomas. [0003] According to pathology and staining, pituitary tumors are divided into four types: non-granular and non-functional chromophobe tumors, granular and functional oncocytomas and basophil tumors, and mixed types. In recent years, pituitary tumors have been divided into prolactinomas, somatotropinomas, corticotropinomas, thyrotropinomas, gonadotropinomas, multihormonal adenomas, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/68G01N33/574A61K45/00A61P35/00
Inventor 宋宏涛杨承刚孙耀兰
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com