High static pressure and enzyme hydrolysis combined method for preparing hypoallergenic ginkgo protein powder

A technology with high static pressure combined with enzymes, applied in food science, fermentation, etc., to achieve the effects of high safety, good solubility, and simple process operation

Inactive Publication Date: 2016-07-13
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no research report on the application of high static pressure and proteolysis technology to ginkgo protein desensitization at home and abroad

Method used

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  • High static pressure and enzyme hydrolysis combined method for preparing hypoallergenic ginkgo protein powder
  • High static pressure and enzyme hydrolysis combined method for preparing hypoallergenic ginkgo protein powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] High static pressure treatment of ginkgo protein

[0034] Take a certain amount of ginkgo protein, prepare a ginkgo protein solution with a concentration of 40-60 mg / mL with distilled water, seal it in a double-layer polyethylene plastic bag, vacuum seal it, put it in a high-pressure device, and store it at 25°C. Under high static pressure treatment, the treatment pressure range is 100-700MPa, and the treatment time is 20-30min.

[0035] The present invention studies the influence of different treatment pressures on the sensitization of ginkgo protein. 50 g of ginkgo protein is taken respectively, and distilled water is used to prepare a ginkgo protein solution with a concentration of 50 mg / mL, which are respectively sealed in double-layer polyethylene plastic bags, and pumped out. After being vacuum-sealed, place it in a high-pressure equipment, and use 100, 200, 300, 400, 500, 600, and 700 MPa to perform high-static pressure treatment at 25°C for 20 minutes, and then us...

Embodiment 2

[0038] Protease Hydrolysis of Ginkgo Protein

[0039] Take a certain amount of high-pressure-treated ginkgo protein sample, heat-treat it at 90°C for 5 minutes, and then use 1mol / L HCl or 1mol / L NaOH to adjust the pH value of the solution to the optimum hydrolysis pH for protease. According to the quality of ginkgo protein sample, press 1~ Add protease to 5% mass ratio for hydrolysis. The hydrolysis temperature is the optimum hydrolysis temperature of protease. The hydrolysis time is 1-2 hours. After hydrolysis, the enzyme activity is inactivated at 95°C for 10 minutes. Ginkgo protein powder.

[0040] The protease is one of alkaline protease, papain, pepsin or neutral protease.

[0041] The optimum hydrolysis pH of the protease is pH 8.0 for alkaline protease, pH 6.5 for papain, pH 1.5 for pepsin and pH 7.0 for neutral protease.

[0042] The optimum hydrolysis temperature for protease is 45°C for alkaline protease, 55°C for papain, 40°C for pepsin and 45°C for neutral protea...

Embodiment 3

[0046] Preparation of Hypoallergenic Ginkgo Protein Powder

[0047] (1) Separation and purification of ginkgo protein

[0048] Take 1kg of fresh ginkgo fruit, shell them, freeze-dry them, grind and sieve them, and degrease them with petroleum ether until the degreasing is complete. After the petroleum ether is completely volatilized, add pH7.4 at a ratio of 1:15g / mL to a concentration of 0.2mol / L The Tris-HCl solution was used for protein extraction, magnetically stirred at 4°C for 24h, then refrigerated and centrifuged at 5000r / min for 15min at 4°C, and the supernatant was passed through 40 and 80g / 100mL saturated (NH4) 2 SO 4 Carry out fractional precipitation, refrigerate and centrifuge at 5000r / min for 30min at 4°C, dissolve the precipitate with a small amount of Tris-HCl solution with a concentration of 0.01mol / L at pH 8.0, place it in a dialysis bag with a molecular weight cut-off of 3.5kDa and use the same Tris- HCl solution was dialyzed, and after the dialysate was f...

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Abstract

The invention relates to a high static pressure and enzyme hydrolysis combined method for preparing hypoallergenic ginkgo protein powder. Fresh ginkgoes are shelled, crushed, extracted with a Tris-HCl solution and subjected to fractional precipitation with saturated (NH4)2SO4, ginkgo protein is prepared, a ginkgo protein solution with certain concentration is prepared from the ginkgo protein with distilled water and sealed in a double-layer polyethylene plastic bag, the double-layer polyethylene plastic bag is put in high-pressure equipment and treated under high static pressure for 20-30 min after vacuum pumping seal is performed, a ginkgo protein sample treated under the high pressure is taken and subjected to heat treatment at the temperature of 90 DEG C for 5 min, the pH value of the solution is adjusted until the pH is most suitable for hydrolysis of protease, protease is added in a certain proportion to be hydrolyzed for 1-2 h, the hydrolysis temperature is the best hydrolysis temperature for protease, enzyme denaturalixation is performed at the temperature of 95 DEG C for 10 min after hydrolysis ends, and the hypoallergenic ginkgo protein powder is obtained after the hydrolyzed sample is freeze-dried. The ginkgo protein powder prepared with the technology is hypoallergenic or doesn't cause allergy and has good solubility, emulsibility and antioxidant activity and high added value, and the method can be a new technology for producing the hypoallergenic ginkgo protein powder in the industry.

Description

technical field [0001] The invention belongs to the technical field of agricultural and forestry product processing, and in particular relates to a method for preparing low-sensitivity ginkgo protein powder by using ginkgo protein as a raw material and adopting high static pressure combined with enzymatic hydrolysis. Background technique [0002] Ginkgo is the fruit of ginkgo biloba, a unique ancient tree species in China. my country is rich in ginkgo resources, and its output accounts for 70% of the world's total. Ginkgo has extremely high nutritional and medical value, and it has a history of more than 1,000 years as a dietotherapy, nourishing, and health food. Due to the rapid development of ginkgo in recent years, serious market supply and demand problems have emerged. Experts predict that according to the planting conditions of ginkgo production areas in various parts of my country, the national ginkgo production will reach 300,000 tons in 10 years, while the internati...

Claims

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Application Information

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IPC IPC(8): C12P21/06A23L33/185
CPCC12P21/06
Inventor 王成章周昊陈虹霞
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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