Genipin cross-linked biogel as well as preparation method and application thereof

A bio-gel and genipin technology, applied in the field of medicine, can solve the problems of poor extensibility and elasticity, and high brittleness of cross-linked bio-gel, and achieve the effects of good mechanical properties, good water absorption, and promotion of wound healing.

Active Publication Date: 2016-07-20
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cross-linked biogel obtained by reacting genipin with polymer materials has high brittleness, poor extensibility and elasticity, and still cannot be used as a wound dressing

Method used

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  • Genipin cross-linked biogel as well as preparation method and application thereof
  • Genipin cross-linked biogel as well as preparation method and application thereof
  • Genipin cross-linked biogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-8

[0044] A series of genipin cross-linked biogels without any drug were prepared according to the above method, and the composition of the raw materials is shown in Table 1.

[0045] Table 1 The raw material composition of genipin cross-linked biogel

[0046]

[0047] Simultaneously, also prepared the biological gel that does not contain any medicine of two comparative examples according to the above-mentioned method, its raw material composition is consistent with embodiment 4, only the final concentration of genipin in the gel solution is respectively 0.01wt% ( Comparative Example 1) and 0.5 wt% (Comparative Example 2).

[0048] Determination of the performance of the genipin cross-linked biogel prepared in Table 1

[0049] 1. Microstructure:

[0050] The genipin cross-linked biogel prepared according to the raw materials in Table 1 was pre-frozen in a -80°C refrigerator for 24 hours, freeze-dried for 48 hours, and the internal microstructure of the cut surface was invest...

Embodiment 9

[0068] Embodiment 9: Genipin cross-linked biogel loaded with antibacterial drugs

[0069] The method for preparing the genipin cross-linked biogel loaded with antibacterial drugs comprises the following steps:

[0070] (1), the preparation of genipin solution:

[0071] 5g of genipin was weighed and dissolved in 95g of deionized water, and mixed uniformly to obtain a genipin solution with a mass percentage of 5wt%.

[0072] (2), the preparation of gel solution:

[0073]Disperse the matrix in 1wt% acetic acid aqueous solution, after fully swelling, add an appropriate amount of 5wt% genipin solution and 20wt% gentamycin sulfate solution, stir evenly, and dilute with deionized water to obtain a gel solution, genipin The final concentration of flat in the gel solution is 0.05wt%-0.2wt%, the final concentration of matrix in the gel solution is 1wt%-10wt%, the final concentration of gentamicin sulfate in the gel solution is 0.001wt %-10wt%.

[0074] (3), forming of genipin cross-...

Embodiment 10-13

[0076] Embodiment 10-13: Genipin cross-linked biogel loaded with antibacterial drugs

[0077] The preparation method is the same as in Example 9, except that the gentamicin sulfate solution in the step (2) is replaced with a nano-silver sulfadiazine suspension, and the final concentration of the nano-silver sulfadiazine in the gel solution is 0.001wt%-10wt%. .

[0078] Wherein the preparation of the nano-silver sulfadiazine suspension of 20wt% is: dissolving and dispersing 2.5ml Tween 80 in 50g deionized water to obtain a Tween 80 solution, dispersing 10g silver sulfadiazine in the Tween 80 solution and shearing evenly , put into a ball mill, adjust the rotating speed to 1500rpm for 10min, and then 2000rpm for 50min to obtain nano-silver sulfadiazine suspension.

[0079] A series of genipin cross-linked biogel examples 10-13 loaded with antimicrobial drugs were prepared according to the method of example 9. The gel raw material was composed of examples 1-4. The final concent...

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Abstract

The invention discloses genipin cross-linked biogel as well as a preparation method and application thereof. The genipin cross-linked biogel comprises the following raw materials in percentage by weight: 0.05wt%-0.2wt% of genipin, 1wt%-10wt% of matrix and the balance of water. By carrying out crosslinking by virtue of optimized genipin concentration, the obtained biogel has the properties of low toxicity, good water-absorbing property and mechanical property and the like. The biogel can be directly used for protecting and isolating the surfaces of various wounds, bedsores and ulcers, can be used as a dressing carrier to load antibacterial drugs, paregorics, growth factors and the like, and can be applied to the treatment of wound infection, wound analgesia or promotion of wound healing and the like. According to the genipin cross-linked biogel, the matrix, a crosslinking agent and the components are all derived from natural organisms, so that the biogel is safe and non-toxic and can be widely applied to the treatment and repair of clinical acute and chronic wounds.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a biogel cross-linked by using genipin as a cross-linking agent and a preparation method and application thereof. Background technique [0002] Trauma refers to skin damage caused by any physical factor, chemical factor or disease infection, including anatomical and functional damage. According to the natural healing process after trauma, trauma can be divided into acute trauma and chronic trauma. Acute trauma refers to wounds that generally heal within 8-12 weeks, most of which are caused by mechanical injuries caused by physical factors, such as abrasions, knife wounds, etc., and also include burns or corrosion injuries caused by some radiation or chemical substances . Chronic wounds generally refer to wounds that heal slowly, usually for more than 1 year, and may recur. The delay in healing may be caused by different factors such as the presence of complications such as di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24C08L5/08C08L89/00C08L33/26A61K9/06A61K31/167A61K31/7036A61K38/18A61K47/36A61K47/42A61P17/02A61P29/00A61P31/02A61L26/00
CPCA61K9/06A61K31/167A61K31/7036A61K38/1808A61K47/36A61K47/42A61L26/0023A61L26/0066A61L26/008A61L2300/402A61L2300/404A61L2300/414C08J3/075C08J3/24C08J2305/08C08J2333/26C08J2389/00C08L5/08C08L33/26C08L89/00C08L2312/00
Inventor 窦桂芳高磊甘慧孟志云孙涛朱晓霞顾若兰吴卓娜孙文种李俭郑颖
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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