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Liquid composite lactic acid bacteria fermentation agent and preparation method and application thereof

A compound lactic acid bacteria and starter technology, applied in biochemical equipment and methods, bacteria used in food preparation, applications, etc., can solve the problems of limited types of harmful microorganisms, and it is difficult to widely inhibit the accumulation of harmful microbial biogenic amines. Improved safety and simple process

Inactive Publication Date: 2016-07-20
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are still some defects in the technology of inoculating harmful microorganisms inhibited by lactic acid bacteria, such as: using a single lactic acid bacteria to inhibit harmful microorganisms, the types of harmful microorganisms that can be inhibited are limited, and it is difficult to widely inhibit harmful microorganisms and control the accumulation of various biogenic amines In addition, the current lactic acid bacteria inoculation conditions are difficult to quickly establish population dominance in solid-state fermented fish products

Method used

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  • Liquid composite lactic acid bacteria fermentation agent and preparation method and application thereof
  • Liquid composite lactic acid bacteria fermentation agent and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0032] Preparation of lactic acid bacteria starter: use MRS solid medium plate to activate low-temperature stored Pediococcus pentosaceus and Lactobacillus acidophilus at 25°C. Use lactic acid bacteria fermentation liquid medium (each 100 ml of medium contains 10 g of sodium chloride, 15 g of lactose, 20 g of sea bass homogenate supernatant, 0.22 micron membrane filter sterilization) to cultivate first-level seeds and second-level seeds, Use the filtered medium to dilute the secondary seeds of the two bacteria to 10 7 cfu / mL, mixed with equal volume before inoculation as a compound starter.

[0033] Fermentation of sea bass: 5 sea bass (approximately 750 g / piece) are washed, eviscerated and gills are washed and drained; 10 grams of pepper, 10 grams of aniseed and 200 grams of salt are fried to make flour and then evenly sprinkled on the fish body Surface; place the sea bass in a fresh-keeping box, spray 200 ml of compound starter evenly on the surface of the fish body; buckle the...

Embodiment 2

[0041] Preparation of lactic acid bacteria starter: use MRS solid medium plates to activate low-temperature stored Lactobacillus plantarum, Lactobacillus delbrueckii and Bifidobacterium lactis at 25°C. Use lactic acid bacteria fermentation liquid medium (each milliliter of medium contains 10 grams of sodium chloride, 20 grams of sucrose, 0.22 micron filter membrane filter sterilization) to cultivate first-level seeds and second-level seeds, use the filtered medium to dilute the three bacteria respectively Of secondary seeds to 10 7 cfu / mL, mixed with equal volume as a compound starter.

[0042] Fermentation of small yellow croaker: Wash 5 kg of small yellow croaker, remove the internal organs and heads, wash and drain; add 500 grams of salt and mix well and place in a porcelain jar; cover the porcelain jar and seal it and ferment at 15°C for 20 days without adding Compound starter, as a control group; add 500 ml of inoculation group 1 to a concentration of 10 7 cfu / mL Lactobacill...

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Abstract

The invention relates to the technical field of microorganism fermentation agent culture, in particular to a lactic acid bacteria composite fermentation agent, a preparation method and application of the lactic acid bacteria composite fermentation agent in lowering biogenic amine accumulation in fermented fish products.The liquid composite lactic acid bacteria fermentation agent comprises two or three of lactobacillus plantarum, pediococcus pentosaceus, lactobacillus bifidus, lactobacillus acidophilus and lactobacillus debrueckii.The preparation method of the liquid composite lactic acid bacteria fermentation agent comprises the following steps of preparation of a fermentation medium, lactic acid liquid culture, preparation of the liquid composite lactic acid bacteria fermentation agent and the like.A fish body to be fermented can be coated with the liquid composite lactic acid bacteria fermentation agent before being fermented, conventional condition fermentation is carried out, and fermented fish with low biogenic amine accumulation is obtained.The concentration of biogenic amines such as histamine, tyramine, spermine, putrescine and cadaverine in the fermented fish final product can be lowered by 50% or above, and the safety of the fermented fish product is improved; the technology is simple, and industrial production and application can be achieved.

Description

Technical field [0001] The invention relates to the technical field of microbial starters, in particular to a lactic acid bacteria composite starter, a preparation method and its application in reducing the accumulation of bioamines in fermented fish products. Background technique [0002] Fermented fish is a fish processing product with a long history in my country. Fresh fish has stable quality and extended shelf life after microbial fermentation. The nutritional value is preserved, and it can also produce unique flavor substances. It is the main component of the diet in coastal areas. Fermentation Whole fish, fish sauce and fish sauce are all fermented fish products. There are a huge variety of microorganisms in naturally fermented fish, many of which contain amino acid decarboxylase or arginine deiminase, which decompose free amino acids to produce histamine, tyramine, spermine, putrescine and other biogenic amines , Bring safety hazards to this kind of food. Histamine and t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L17/00A23L33/135C12R1/25C12R1/23C12R1/225C12R1/01
CPCC12N1/20A23V2002/00A23V2400/137A23V2400/427A23V2400/113A23V2400/51A23V2400/169A23V2200/30
Inventor 纪超凡韩静林心萍董秀萍孙黎明姜鹏飞刘洒洒孙悦
Owner DALIAN POLYTECHNIC UNIVERSITY
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