Preparation method and application of a ractopamine electrochemical bioimmunosensor
A technology of ractopamine and biological immunity, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by making materials undergo chemical reactions, can solve the problems affecting the convenience of operation and restricting wide application, etc., to achieve Easy operation, high selectivity detection, simple preparation effect
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Embodiment 1
[0041] Example 1 A ractopamine electrochemical bioimmunosensor, the specific preparation steps are:
[0042] (1) Preparation of ECL working electrode W1:
[0043] 1) Use ITO conductive glass as the working electrode, and drop-coat NH on the surface of the electrode 4 NiPO 4 Solution, covering an area of 1 cm × 1 cm, dry at room temperature;
[0044] 2) Apply the working electrode obtained in 1) to the NH 4 NiPO 4 Surface drop coating of Au@Ag 2 S NRs sol, covering an area of 1 cm × 1 cm, dried at room temperature;
[0045] 3) Immerse the working electrode obtained in 2) into the electrolyte, and the immersion area is NH 4 NiPO 4 and Au@Ag 2 The area covered by S NRs was electrochemically deposited on the working electrode using a three-electrode system. After deposition, the working electrode was taken out, washed with ultrapure water, and dried at 4 °C in the dark to prepare the electrochemiluminescence working electrode W1;
[0046] The NH 4 NiPO 4 The solutio...
Embodiment 2
[0064] Example 2 A ractopamine electrochemical bioimmunosensor, the specific preparation steps are:
[0065] (1) Preparation of ECL working electrode W1:
[0066] The preparation steps are the same as the preparation steps of W1 in Example 1, the difference is: the ammonium salt addition is 0.3 g in the preparation step of the nickel ammonium phosphate micro-nano material, the phosphate addition is 0.20 g, and the nickel dichloride addition is 0.20 g, the stirring temperature and time were 38 ℃ and 12 h, respectively; the length of gold nanorods was 30 nm; the concentration of luminol in the electrolyte was 5 mmol / L, and the concentration of sulfuric acid was 0.5 mol / L; cyclic voltammetry When performing electrochemical deposition, cycle 25 times;
[0067] (2) Preparation of photoelectrochemical working electrode W2:
[0068] 1) Use ITO conductive glass as the working electrode, drop-coat 10 µL TiO on the surface of the electrode 2 NSs sol, dry at room temperature;
[006...
Embodiment 3
[0074] Example 3 A ractopamine electrochemical bioimmunosensor, the specific preparation steps are:
[0075] (1) Preparation of electrochemiluminescence working electrode W1:
[0076] The preparation steps are the same as the preparation steps of W1 in Example 1, the difference is: the ammonium salt addition is 0.4 g in the preparation step of the nickel ammonium phosphate micro-nano material, the phosphate addition is 0.25 g, and the nickel dichloride addition is 0.25 g, the stirring temperature and time were 45 ℃ and 14 h, respectively; the length of gold nanorods was 40 nm; the concentration of luminol in the electrolyte was 10 mmol / L, and the concentration of sulfuric acid was 1.0 mol / L; cyclic voltammetry When performing electrochemical deposition, cycle 30 times;
[0077] (2) Preparation of photoelectrochemical working electrode W2:
[0078] 1) Using ITO conductive glass as the working electrode, drop-coat 12 µL TiO on the surface of the electrode 2 NSs sol, dry at r...
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