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Plant water culture method and special device thereof

A plant water and hydroponics technology, applied in the direction of botany equipment and methods, cultivation, application, etc., can solve problems such as difficult to ensure root elongation and growth, difficult growth cycle, large water consumption, etc., to prevent algae growth, Space-saving and water-saving, and the effect of preventing the generation of bacteria

Inactive Publication Date: 2016-07-27
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most hydroponic systems use large amounts of water and are prone to bacteria. On the one hand, it is difficult to ensure the normal elongation and growth of roots, and on the other hand, it is difficult to ensure that they complete a complete growth cycle.

Method used

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  • Plant water culture method and special device thereof
  • Plant water culture method and special device thereof
  • Plant water culture method and special device thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the preparation of plant hydroponic device

[0054] 1. Prepare an incubation chamber with a micropipette tip and a sponge.

[0055] The structure diagram of the cultivation cavity is shown in image 3 . It consists of a cavity 2-1 and a supporting medium 2-2.

[0056] The preparation method of the cultivation cavity is as follows:

[0057] (1) The structural schematic diagram of the tip of the micropipette is shown in figure 1 , consisting of a base and a pointed head. Take the tip of the micropipette, cut off the tip, and keep the base, which is the cavity. The structure diagram of the cavity is shown in figure 2 . The cavity is an inverted frustum of circular shape, the inner diameter of the upper bottom is 0.4 cm, the outer diameter is 0.6 cm, the inner diameter of the lower bottom is 0.3 cm, the outer diameter is 0.5 cm, and the height is 0.5 cm.

[0058] (2) Fill the cavity with a sponge. A part of the sponge is filled in the cavity, and the ...

Embodiment 2

[0064] Embodiment 2, the application of plant hydroponic device

[0065] The plant hydroponic device prepared in Example 1 cultivated Arabidopsis (Columbia ecotype Arabidopsis or nla single mutant Arabidopsis, cultured respectively), and the Hoagland nutrient solution was used as the hydroponic solution on the first to 23 days of the test (i.e. step 2), start group culture from the 24th day of the test (one group uses Hoagland nutrient solution as the hydroponic solution, and the other group uses low-nitrogen nutrient solution as the hydroponic solution) (i.e. step 3), the specific steps are as follows:

[0066] 1. Seed sterilization and vernalization

[0067] Get the mature seeds of Arabidopsis thaliana, rinse with 75% (volume ratio) ethanol aqueous solution, then rinse with sterile water, then rinse with 1.5% (volume ratio) sodium hypochlorite aqueous solution, then rinse with sterile water, and then vernalize (Store at 4°C for 2-4 days).

[0068] 2. Take the plant hydropo...

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Abstract

The invention discloses a plant water culture method and a special device thereof and provides a plant water culture device. The plant water culture device comprises a water culture plate (1) and a plurality of culture cavities (2); each culture cavity is composed of a cavity body (2-1) and a supporting medium (2-2); each cavity body is in the shape of a hollow pipe with an upper opening and a lower opening; each cavity body is filled with a part of the corresponding supporting medium (2-2), and the other part of the supporting medium (2-2) extends out of the lower opening of each cavity body; a plate body of the water culture plate is provided with a plurality of through holes; a suspended supporting component higher than the plate body plane of the water culture plate is arranged on the edge of the water culture plate; each culture cavity is inserted in one through hole. The method adopted for plant culture has the advantages of low cost, easy operation and the like. Through the method and the device, assistance can be provided for scientific research to be carried out smoothly, and certain help can be provided for botany, plant physiology and molecular biology.

Description

technical field [0001] The invention relates to a plant hydroponic method and a special device thereof. Background technique [0002] Arabidopsis has the characteristics of short growth cycle, small genome and easy transformation, so it is widely used as a model plant in the molecular biology research of higher plants. The growth of Arabidopsis is restricted by external conditions such as light, temperature, water, and nutrition, and its growth status directly affects the progress of scientific research. Due to different research purposes, various culture systems are used for the research of Arabidopsis thaliana, such as agar powder plate, normal soil culture, sand culture and hydroponics, etc. [0003] Hydroponics is a standardized, uniform and highly repeatable culture system, which is widely used in the analysis and interaction research of Arabidopsis root system and nutrient components due to its high nutrient utilization rate and controllable nutrient components. In t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/02A01G31/00
CPCA01G31/00A01G31/02Y02P60/21
Inventor 李文学刘文文赵勐
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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