Polylactic acid-hydroxyapatite whisker compound porous scaffold for bone tissue engineering and preparation method thereof

A technology of hydroxyapatite and bone tissue engineering, applied in the field of biomedical materials, can solve problems such as lower requirements, and achieve the effects of improving mechanical strength, excellent biocompatibility, and good osteoconductivity

Inactive Publication Date: 2016-07-27
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The preparation methods of porous scaffold materials mainly include phase separation-freeze drying, solvent casting-particle leaching, solid free forming technology, microsphere sintering, fiber bonding and gas foaming, among which solvent casting-particle leaching Because of its low requirements on test equipment and experimental conditions, and many advantages such as controllable porosity and pore size of the scaffold, it has become a commonly used preparation method for tissue engineering porous scaffolds, and is widely used in the preparation of porous bone tissue engineering scaffolds. The solvent casting-particle leaching method also has obvious disadvantages. For example, the volume of the scaffold shrinks during the solvent volatilization process, so that the traditional solvent casting-particle leaching method is only suitable for preparing thin-layer porous membranes. Therefore, the preparation method of large-volume porous scaffolds Research and development have extremely high practical significance for the research and application of porous bone tissue engineering scaffolds

Method used

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  • Polylactic acid-hydroxyapatite whisker compound porous scaffold for bone tissue engineering and preparation method thereof
  • Polylactic acid-hydroxyapatite whisker compound porous scaffold for bone tissue engineering and preparation method thereof
  • Polylactic acid-hydroxyapatite whisker compound porous scaffold for bone tissue engineering and preparation method thereof

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Experimental program
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Embodiment 1

[0029] The polylactic acid / hydroxyapatite whisker composite porous scaffold for bone tissue engineering described in this example, the hydroxyapatite whisker accounts for 0.5wt% of the total amount of polylactic acid and hydroxyapatite whisker, the pores of the composite porous scaffold The ratio is 91.1%, and the diameter of the irregular through-holes is 100-500 μm.

[0030] The preparation method of the polylactic acid / hydroxyapatite whisker composite porous scaffold for bone tissue engineering in this example, the specific steps are as follows:

[0031] (1) According to the ratio of pore-forming agents sodium chloride and potassium chloride to 92% of the total volume of sodium chloride, potassium chloride and polylactic acid, add the mixture of pore-forming agents sodium chloride and potassium chloride to polylactic acid In the organic solvent of chloroform, fully stir to make it uniformly dispersed, wherein the particle size of sodium chloride and potassium chloride is 10...

Embodiment 2

[0043] The polylactic acid / hydroxyapatite whisker composite porous scaffold for bone tissue engineering described in this embodiment, the hydroxyapatite whisker accounts for 10wt% of the total amount of polylactic acid and hydroxyapatite whisker, the porosity of the composite porous scaffold It is 80.0%, and the diameter of the irregular through hole is 50-400μm.

[0044] The preparation method of the polylactic acid / hydroxyapatite whisker composite porous scaffold for bone tissue engineering in this example, the specific steps are as follows:

[0045] (1) According to the ratio of the pore-forming agent potassium chloride to 80.5% of the total volume of potassium chloride and polylactic acid, the pore-forming agent potassium chloride is added to the mixed solvent of dichloromethane and chloroform of polylactic acid, fully Stir to disperse evenly, wherein the particle size of potassium chloride is 50-400 μm;

[0046] (2) Add hydroxyapatite whiskers to the mixture prepared in ...

Embodiment 3

[0052] The polylactic acid / hydroxyapatite whisker composite porous scaffold for bone tissue engineering described in this embodiment, the hydroxyapatite whisker accounts for 50wt% of the total amount of polylactic acid and hydroxyapatite whisker, the porosity of the composite porous scaffold It is 45.8%, and the diameter of the irregular through hole is 100-600μm.

[0053] The preparation method of the polylactic acid / hydroxyapatite whisker composite porous scaffold for bone tissue engineering in this embodiment, the specific steps include the following:

[0054] (1) According to the ratio of the pore-forming agent calcium sulfate to 50% of the total volume of calcium sulfate and polylactic acid, add the pore-forming agent to the carbon tetrachloride organic solvent of polylactic acid, stir well to disperse it evenly, and calcium sulfate The particle size is 100-600μm;

[0055] (2) Add hydroxyapatite whiskers to the mixture prepared in step (1) according to the proportion of ...

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Abstract

The invention relates to a polylactic acid-hydroxyapatite whisker compound porous scaffold for bone tissue engineering and a preparation method thereof, and belongs to the field of biomedical material technique application. The compound porous scaffold is prepared from, by weight, 0.5%-50% of hydroxyapatite whiskers and 50%-99.5% of polylactic acid. The compound porous scaffold is prepared by adopting a solvent casting-vacuum volatilizing-particle leaching method, the technology is simple, operation is convenient, and the yield is stable; according to the prepared compound porous scaffold, the porosity is 45.8%-91.1%, the diameter of irregular straight-through holes is 50-600 micrometers, the compressive strength is 1.2-19.1 MPa, the cell toxicity is qualified, and the good clinical application prospect is achieved.

Description

technical field [0001] The invention relates to a polylactic acid / hydroxyapatite whisker composite porous scaffold for bone tissue engineering and a preparation method thereof, belonging to the technical application field of biomedical materials. Background technique [0002] The basic method of bone tissue engineering is to plant the isolated autologous high-concentration osteoblasts, bone marrow stromal cells or chondrocytes in a natural or synthetic biocompatible and On the cell scaffolds gradually degraded and absorbed by the human body, this cell scaffold material provides cells with a three-dimensional living space, which is beneficial for cells to obtain sufficient nutrients, perform gas exchange, and eliminate waste materials, so that cells can grow according to the prefabricated three-dimensional scaffold, and then The cell-scaffold material complex is implanted into the bone defect site, and while the material is gradually degraded, the implanted bone cells continu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/56A61L27/58
CPCA61L27/46A61L27/56A61L27/58
Inventor 颜廷亭吴思宇陈庆华
Owner KUNMING UNIV OF SCI & TECH
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