Fluorescent pseudomonas, method for producing phenazine amide and application of phenazine amide
A technology based on Pseudomonas fluorescens and phenazine amide, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of large dosage and low output of phenazine carboxylic acid, and achieve increased yield , high yield effect
Active Publication Date: 2016-07-27
SUNGEN BIOSCIENCE CO LTD
View PDF2 Cites 3 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, phenazine carboxylic acid has the problems of low yield and large dosage as a biopesticide
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
preparation Embodiment 2 to 5
[0070] Preparation Examples 2 to 5 shake flask fermentation
[0071] Carry out shake flask fermentation according to the method for preparing embodiment 1, difference is that fermentation medium is respectively:
preparation Embodiment 2
[0072] Preparation Example 2: glycerin 10g / L, sucrose 10g / L, peptone F40320g / L, ammonium nitrate 0.5g / L, magnesium sulfate 1g / L, dipotassium hydrogen phosphate 1g / L, vitamin B 1 10mg / L, ferric chloride 2mg / L, manganese sulfate 5mg / L, zinc sulfate 2.5mg / L, potassium iodide 0.7mg / L, boric acid 1.4mg / L, pH7.2.
preparation Embodiment 3
[0073] Preparation Example 3: Glucose 10g / L, sucrose 10g / L, peptone 20g / L, ammonium nitrate 0.5g / L, magnesium sulfate 1g / L, dipotassium hydrogen phosphate 1g / L, vitamin B 1 10mg / L, pH7.2.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides fluorescent pseudomonas sp.ST16 with collection number of CGMCC No.2397 and a method for producing phenazine amide. The method comprises the steps of culturing fluorescent pseudomonas CGMCC No.2397 in a culture medium so that phenazine compounds are generated and gathered in strain cells and the culture medium, recovering phenazine amide from the strain cells and the culture medium and purifying phenazine amide. Phenazine amide provided by the invention can be used in agricultural production as a biopesticide and has better effects than phenazine carboxylic acid.
Description
technical field [0001] The invention relates to a pseudomonas fluorescens CGMCC No.2397, a method for producing phenazinamide and the application of the phenazinamide prepared by the method in biological pesticides. Background technique [0002] The production methods of phenazine compounds mainly include chemical synthesis and biological fermentation. The chemical synthesis method was first reported in the literature in 1930, mainly including anthranilic acid / nitrobenzene method, 1-methylphenazine oxidation method, 1-cyanophenazine hydrolysis method and 2-(anilino)isodi Benzoic acid method, etc. The chemical method has low yield, high cost, and great environmental pollution, making it difficult to industrialize. [0003] Biological fermentation is divided into traditional methods and improved methods. The extraction process of the traditional fermentation method is cumbersome, high energy consumption, and low yield. The improved fermentation method adopts the membrane i...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/12C07D241/46C12R1/39
Inventor 陈杰鹏段丽丽邱雪莲陈鸿锐纪烨瑜陈煜藩蔡桂珠洪琳黄晓莹陈倩莹许志锴
Owner SUNGEN BIOSCIENCE CO LTD