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Functionalized ionic liquid-modified carbon nanotube and method for immobilizing lipase through carbon nanotube

A technology of ionic liquids and carbon nanotubes, applied in the direction of fixing on or in inorganic carriers, fixing on/in organic carriers, nanotechnology, etc., can solve the problem of weak binding force between enzyme molecules and carriers, immobilization Reduce efficiency and stability, pollute catalytic reaction products and other problems, achieve simple apparent activity and repeated use effect, improve enzymatic properties, and cheap price

Inactive Publication Date: 2016-08-10
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although enzymes can be directly adsorbed on carbon nanotubes through physical adsorption, the binding force between enzyme molecules and the carrier is not strong, which easily leads to a decrease in the immobilization efficiency and stability of the enzyme, and the enzyme is easy to fall off from the carrier and contaminate the catalytic reaction products, etc.

Method used

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  • Functionalized ionic liquid-modified carbon nanotube and method for immobilizing lipase through carbon nanotube
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  • Functionalized ionic liquid-modified carbon nanotube and method for immobilizing lipase through carbon nanotube

Examples

Experimental program
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Effect test

Embodiment 1

[0048] (1) Purification and oxidation of carbon nanotubes: take 1 g of original carbon nanotubes and stir in 2.6M nitric acid solution for 24 hours under reflux. Add 10 mg of purified carbon nanotubes to 40 ml of concentrated nitric acid and concentrated sulfuric acid mixture, ultrasonicate for 3 hours, add water to dilute, filter and wash until neutral, vacuum dry overnight, grind for later use, and obtain carboxylated carbon nanotubes (MWCNTs-COOH).

[0049](2) Preparation of functionalized ionic liquid-modified carbon nanotube immobilized enzyme carrier: a certain molar ratio of 1-methylimidazole and 3-bromopropylamine hydrobromide was mixed in absolute ethanol solvent and refluxed for 24 hours. The reaction product was dissolved with a small amount of water after removal of solvent and volatile unreacted substances, and potassium hydroxide was added to adjust the pH to 8.0. The resulting solution was distilled off under reduced pressure to remove most of the water, then dr...

Embodiment 2

[0062] 1) Purification and oxidation of carbon nanotubes: same as described in Example 1.

[0063] 2) Preparation of functionalized ionic liquid-modified carbon nanotube-immobilized enzyme carrier: a certain molar ratio of 1-ethylimidazole and 3-bromopropylamine hydrobromide was mixed in absolute ethanol solvent, and refluxed for 24 hours. The reaction product was dissolved with a small amount of water after removal of solvent and volatile unreacted substances, and potassium hydroxide was added to adjust the pH to 8.0. The resulting solution was distilled off under reduced pressure to remove most of the water, then dried in vacuo to constant weight, and the product was extracted with a mixed solvent of ethanol-tetrahydrofuran, the filtrate was collected and distilled under reduced pressure to recover the solvent, and then dried in vacuo to constant weight to obtain a yellow viscous liquid 1-(1 -aminopropyl)-3-ethylimidazolium bromide (IL-NH 2 ). MWCNTs-COOH, IL-NH 2 1. DCC ...

Embodiment 3

[0067] 1) Purification and oxidation of carbon nanotubes: same as described in Example 1.

[0068] 2) Preparation of functionalized ionic liquid-modified carbon nanotube-immobilized enzyme carrier: a certain molar ratio of 1-butylimidazole and 3-bromopropylamine hydrobromide was mixed in absolute ethanol solvent, and refluxed for 24 hours. The reaction product was dissolved with a small amount of water after removal of solvent and volatile unreacted substances, and potassium hydroxide was added to adjust the pH to 8.0. The resulting solution was distilled off under reduced pressure to remove most of the water, then dried in vacuo to constant weight, and the product was extracted with a mixed solvent of ethanol-tetrahydrofuran, the filtrate was collected and distilled under reduced pressure to recover the solvent, and then dried in vacuo to constant weight to obtain a yellow viscous liquid 1-(1 -aminopropyl)-3-butylimidazolium bromide (IL-NH 2 ). MWCNTs-COOH, IL-NH 2 , DCC w...

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Abstract

The invention provides a functionalized ionic liquid-modified carbon nanotube and a method for immobilizing lipase through the carbon nanotube. According to the method, functionalized ionic liquid with functional groups such as the alkyl group, the carboxyl group, the amino group and the hydroxyl group is introduced to the surface of the carbon nanotube to generate electrostatic interaction, hydrogen-bond interaction, hydrophobic interaction, covalent interaction and the like with amino acid residues of enzyme molecules, the binding force between carriers and enzymes is enhanced, the microenvironment where the enzymes are located is changed, and therefore the catalytic performance of the immobilized enzymes is improved. The functionalized ionic liquid-modified carbon nanotube is used for immobilization of the lipase, and innovation of the lipase immobilization method is achieved.

Description

technical field [0001] The invention belongs to the field of immobilized enzyme technology and catalyst carrier, and relates to the application of functionalized ionic liquid modified carbon nanotubes in the immobilization of lipase. Background technique [0002] Lipase (EC3.1.1.3) can catalyze natural substrates for oil hydrolysis, and is widely used in medicine, chemical industry, food, feed and energy industries, and has become one of the most researched enzyme catalysts. [0003] Since the chemical nature of lipase is a soluble protein, free lipase will encounter a series of problems in industrial applications. For example, the free enzyme in the aqueous phase system is difficult to separate after the catalytic reaction; the catalytic performance is easily affected by other factors such as substrates and products during the reaction; the operating conditions are extreme, etc. Compared with free enzymes, immobilized enzymes have the advantages of good stability, reusabil...

Claims

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Application Information

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IPC IPC(8): C01B31/02C12N11/14C12N11/02B82Y40/00
CPCC12N11/02C12N11/14C01P2002/88
Inventor 胡燚万晓梅黄和相欣然陈虹月
Owner NANJING UNIV OF TECH
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