Hydroxyproline fermentation production technology

A technology for hydroxyproline and production process, applied in the field of separation and extraction of amino acids, can solve the problems of environmental pollution, large water consumption, easy oxidation of hydroxyproline, etc., and achieve the effects of eliminating process waste water and improving utilization rate

Active Publication Date: 2016-08-10
TIANJIN JINGYE FINE CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following defects: ① Hydroxyproline is easily oxidized, and the yield is unstable; ② Crude hydroxyproline has many impurities and deep color, which affects the refined yield and product quality
③Large water consumption, large amount of acid and alkali, will produce a large amount of waste residue and waste water in the production process, causing very serious pollution to the environment
It can overcome these defects, make the fermentation production of hydroxyproline in an environment-friendly way, realize zero discharge of waste residue and wastewater in the extraction production process, and completely eradicate the fermentation production process of environmental pollution problems in the production process. to report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Hydroxyproline content 4.58%, pH6.5, residual sugar 0.8%, NH 4 + 300L of 0.5mol / L hydroxyproline fermentation broth was filtered at 45°C with a 220nm polyacetate microporous membrane to obtain 298L of filtrate with 4.58% hydroxyproline content and 2.0kg of filter residue; Concentrate with a nanofiltration membrane with a molecular weight cut-off of 300Da under the pH and pH conditions to obtain 13L of concentrated solution with a solid content of 10% and 285L of permeate with a hydroxyproline content of 4.58%; Concentrate by electrodialysis at ℃ to obtain 9.1% hydroxyproline, NH 4 + 1mol / L electrodialysis concentrate 142L and containing 0.2% hydroxyproline, NH 4 + 0.05mol / L electrodialysis dilute solution 141L, the electrodialysis dilute solution is concentrated with a roll-type reverse osmosis membrane device to obtain 2% hydroxyproline, NH 4 + 0.5mol / L reverse osmosis concentrate 14L and reverse osmosis permeate 127L. Adjust the pH of the electrodialysis concen...

Embodiment 2

[0029] Hydroxyproline content 4.29%, pH6.6, residual sugar 0.8%, NH 4 + 500L of 0.5mol / L hydroxyproline fermentation broth was filtered at 45°C with a 100nm polyacetate microporous membrane to obtain 497L of filtrate with 4.29% hydroxyproline content and 3.1kg of filter residue; Concentrate with a roll-type nanofiltration membrane with a molecular weight cut-off of 300Da under and pH conditions to obtain a solid content of 21L of a concentrated solution of 12% and a permeate of 476L with a hydroxyproline content of 4.29%; adjust the pH value of the permeate to 5.79, and then desalted and concentrated at 60°C with electrodialysis and roll-type reverse osmosis membrane combination device to obtain 284L of reverse osmosis concentrate with a hydroxyproline content of 7.09%; the reverse osmosis concentrate was cooled to within 30min 4 DEG C, then keep the temperature and stand overnight to allow hydroxyproline crystals to precipitate out, collect the crystals by centrifugation, an...

Embodiment 3

[0031] Hydroxyproline content 4.29%, pH6.6, residual sugar 0.8%, NH 4 + 500L of 0.5mol / L hydroxyproline fermentation liquid was subjected to high-speed centrifugation at 15000rpm at 45°C to obtain 497L of supernatant with 4.29% hydroxyproline content and 3.0kg of filter residue; the supernatant was under the same temperature and pH conditions Concentrate with a nanofiltration membrane with a molecular weight cut-off of 290Da to obtain 21L of a concentrated solution with a solid content of 10% and 475L of a permeate with a hydroxyproline content of 4.29%; Concentrate by electrodialysis to obtain 8.3% hydroxyproline, NH 4 +1mol / L electrodialysis concentrate 238L and containing 0.2% hydroxyproline, NH 4 + 0.05mol / L electrodialysis dilute solution 237L, the electrodialysis dilute solution is concentrated with a roll-type reverse osmosis membrane device to obtain 2% hydroxyproline, NH 4 + 24L of 0.5mol / L reverse osmosis concentrate and 213L of reverse osmosis permeate; adjust...

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Abstract

The invention belongs to the technical field of separation and extraction of amino acids, and concretely relates to a hydroxyproline fermentation production technology. A technology for separating hydroxyproline from a special self-fermentation liquid is created through adopting a multi-stage series membrane separation technology and an electrodialysis technology in order to form the brand new hydroxyproline fermentation production technology. The hydroxyproline fermentation production technology can realize zero discharge of waste residues and wastewater in the hydroxyproline fermentation production process, thoroughly and radically solves the environment pollution problem in the hydroxyproline fermentation production process, improves the total yield of hydroxyproline to 70% or above, greatly improves the quality of the hydroxyproline product, and allows the hydroxyproline content of the hydroxyproline product to reach 99% or above.

Description

technical field [0001] The invention relates to the technical field of separation and extraction of amino acids, in particular to a hydroxyproline fermentation production process. Background technique [0002] Hydroxyproline (Hyp) is an imino acid, which is the product of L-proline hydroxylation, and its molecular formula is C 5 h 9 NO 3 . Hydroxyproline is white flaky crystal or crystalline powder, slightly sweet, with a melting point of 274°C, easily soluble in water and slightly soluble in ethanol. According to the position of hydroxylation of hydroxyproline, it can be divided into 3-hydroxyproline (3-Hyp) or 4-hydroxyproline (4-Hyp). Because hydroxyproline has two asymmetric carbon atoms, there are 4 stereoisomers. Trans-4-hydroxyproline is more common in nature. Trans-4-hydroxyproline was first found in animal collagen protein. [0003] Trans-4-hydroxyproline is widely used in medicine, chemical industry, animal feed, nutrition and beauty industry, etc. [0004] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D207/16A23K20/142
CPCC07D207/16
Inventor 赵体金林俊辉彭久合
Owner TIANJIN JINGYE FINE CHEM
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