Preparation method and application of marine polypeptide-zinc chelate

A technology of zinc chelate and polypeptide complex, which is applied in application, food science, animal protein processing, etc., can solve the problems of complex synthesis, low yield, and low zinc content, and achieve high extraction rate, low price, and easy absorbed effect

Inactive Publication Date: 2016-08-17
XIAMEN 139 BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current zinc supplementation agents are complicated to synthesize, low in yield, low in zinc content, accompanied by mild nausea, vomiting, constipation and other adverse reactions, and are not suitable for patients with diabetes, anemia, and prostatic hypertrophy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] A preparation method of marine polypeptide-zinc chelate, comprising the steps of:

[0023] (1) Preparation of marine peptides: First, ultrafinely pulverize marine animals and plants such as seaweed, fish, shrimp and crab, abalone, sea oyster, clam and their leftovers, and then mix the crushed marine animals and plants and their leftovers with water Mix according to mass volume ratio 1:10-50 (g / mL), use 0.5-2wt% NaOH solution or KOH solution to adjust the pH to 8.0-11.0, after ultrasonic extraction for 10-60min, extract in 50-100℃ water bath for 60-120min , filtered, the filtrate was adjusted to pH 2.5-4.0 with hydrochloric acid for protein precipitation, centrifuged at 3000-6000r / min for 5-10min to obtain the precipitated protein, washed with water to neutrality and then centrifuged again, and the obtained precipitate was freeze-dried to obtain marine animals and plants and their leftovers The protein in the solution; then dissolve the obtained protein with water to mak...

Embodiment 1

[0029] (1) Spirulina is first ultrafinely pulverized, mixed with water at a mass volume ratio of 1:30 (g / mL), adjusted to pH 9.0 with 2wt% NaOH solution, ultrasonically extracted for 10 minutes, then extracted in a water bath at 60°C for 100 minutes, Filtrate, adjust the pH of the filtrate to 3.0 with hydrochloric acid for protein precipitation, centrifuge at 4000r / min for 10 minutes to obtain the precipitated protein, wash with water until neutral, then centrifuge again, and freeze-dry the obtained precipitate to obtain the spirulina protein, and the extraction rate reaches 74.16%;

[0030] (2) Then dissolve the obtained spirulina protein with water to make the protein concentration reach 3.0wt%, and adjust the pH of the solution to 9.0, then keep the temperature at 50°C, add 3.0wt% neutral protease, hydrolyze for 90min, and then sterilize Enzyme to make spirulina polypeptide complex solution;

[0031] (3) Add 1mol / L zinc acetate solution to the obtained spirulina polypeptide...

Embodiment 2

[0034] (1) After pulverizing the small yellow croaker, mix it with water at a mass volume ratio of 1:25 (g / mL) to refine the pulp, so that the protein concentration reaches 1.0wt%, and adjust the pH of the solution to 7.0, and then keep the temperature at 40°C. Add 5.0wt% compound protease, hydrolyze for 60 minutes, and then inactivate the enzyme to make a small yellow croaker polypeptide complex solution;

[0035](2) Add 0.5mol / L zinc sulfate solution to the obtained small yellow croaker polypeptide complex solution at a volume ratio of 3:6, stir evenly, adjust the pH to 7.0, continue to react at 30°C for 3.0h, cool down, and centrifuge to remove impurities. Then add ethanol with 4 times the volume of the supernatant and 80wt%, let it stand for 12 hours, centrifuge to remove the supernatant, and dry the resulting precipitate at low temperature to obtain the small yellow croaker polypeptide-zinc chelate;

[0036] (3) The prepared small yellow croaker polypeptide-zinc chelate i...

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Abstract

The invention discloses a preparation method and application of a marine polypeptide-zinc chelate. Firstly, the protein in marine animals and plants and their leftovers is extracted by alkali-dissolving acid precipitation method, or the marine animals and plants and their leftovers are crushed, After refining, add protease to the obtained protein or slurry for enzymolysis, inactivate the enzyme, and prepare a marine polypeptide complex solution; then use inorganic zinc acetate solution or zinc sulfate solution to chelate with marine polypeptide to obtain marine polypeptide-zinc chelation The obtained marine polypeptide-zinc chelate can be further prepared into snack food, nutraceutical or medicine. The preparation process of the present invention is simple; the prepared marine polypeptide-zinc chelate and its products have a unique chelation system and transport mechanism, are safe, non-toxic, easy to be absorbed, and can supplement amino acids and zinc at the same time, and will surely become a zinc supplement It provides a new idea for the application of marine animals and plants and their leftovers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method and application of a marine polypeptide-zinc chelate. Background technique [0002] Zinc is an essential trace element for the human body, and is a component or activator of various enzymes. have an important role. Although zinc sources are very abundant in nature, the absorption and metabolism of zinc in the human body is limited by various factors. Zinc deficiency has become a public health problem in my country and many developing countries. There are two types of zinc supplements approved by the Ministry of Health: inorganic zinc and organic zinc. Early inorganic zinc salts include zinc sulfate, zinc chloride, etc. These salts are easy to absorb moisture, have low bioabsorption rate, unpleasant taste, and have a greater stimulating effect on the gastrointestinal tract, so they are gradually eliminated; organic zinc salts include zinc acetate , ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/165A23J1/04A23J1/00A23J3/04A23J3/14A23J3/34A61K38/01C12P21/06C07K1/36C07K1/34C07K1/30
CPCA23J1/04A23J1/00A23J3/04A23J3/14A23J3/34A23V2002/00A61K38/01C07K1/30C07K1/34C07K1/36C12P21/06
Inventor 刘斌张凤萍刘青
Owner XIAMEN 139 BIOTECH CO LTD
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