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A kind of microbial inoculant for garbage compost fermentation and its preparation method and application

A microbial agent and garbage heap technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unstable natural composting and fermentation process, easy to be affected by the external environment, and decline in waste incineration efficiency. Achieve the effect of improving microbial activity, low cost, and promoting water leaching

Active Publication Date: 2019-02-05
QINGDAO GENYUAN BIOLOGICAL TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] 2. The natural fermentation process is unstable and easily affected by the external environment
Especially in the northern part of my country, the temperature in winter is low, which leads to the extremely low activity of microorganisms in the domestic waste, and the amount of water removal is not enough within the composting time of the process design, the low calorific value of the waste is reduced, and the efficiency of waste incineration is reduced.
[0007] 3. The natural fermentation process is accompanied by the garbage corruption process, which produces a lot of odor and affects air quality

Method used

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  • A kind of microbial inoculant for garbage compost fermentation and its preparation method and application
  • A kind of microbial inoculant for garbage compost fermentation and its preparation method and application
  • A kind of microbial inoculant for garbage compost fermentation and its preparation method and application

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Embodiment 1

[0041] The microbial agent of the present invention comprises bacillus amyloliquefaciens ( Bacillus amyloloquefaciens ), Candida utilis ( Candida utilis ), Lactobacillus acidophilus ( Lactobacillus acidophilus ) and Rhodopseudomonas palustris ( Rhodopseudanonas palustris ), the Bacillus amyloliquefaciens, Candida utilis, Lactobacillus acidophilus and Rhodopseudomonas palustris described in the present invention are selected from commercially available bacterial agents, wherein the Bacillus amyloliquefaciens can also be preserved in China Bacillus amyloliquefaciens HFJ-7 with the deposit number CGMCC No. 10011 of the General Microbiology Center of the Microbiological Culture Collection Management Committee.

[0042] The depository unit of the Bacillus amyloliquefaciens HFJ-7: General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee; Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology,...

Embodiment 2

[0052] The preparation method of the microbial inoculum that is used for garbage heap fermentation described in the present embodiment specifically comprises the following steps:

[0053] 1. Activate Bacillus amyloliquefaciens, Candida utilis, Lactobacillus acidophilus and Rhodopseudomonas palustris respectively in the corresponding medium, and then carry out seed culture in the respective sterilized medium.

[0054] The culture medium of Bacillus amyloliquefaciens is composed of: 10.0 g of peptone, 3.0 g of beef extract, 20.0 g of glucose, 5.0 g of NaCl, 1000 ml of water, and pH 6.8. The inoculum of Bacillus amyloliquefaciens was 8%, and cultured at 30°C for 24 hours. The bacterial content of Bacillus amyloliquefaciens in the Bacillus amyloliquefaciens bacterial liquid obtained after culturing is 40 billion / ml.

[0055] The Candida utilis culture medium is: 10.0 g of yeast extract, 20.0 g of peptone, 20.0 g of glucose, and 1000 ml of distilled water. The inoculum of Candida...

Embodiment 3

[0061] Collect domestic garbage from a garbage collection station in a residential area in Qingdao City, manually break the bags and mix them evenly for use. The physical composition (mass fraction of wet basis) of domestic waste is 74.1% food, 11.5% paper, 4.3% plastic, and 10.1% other. Its initial water content (wet basis) is 66.2%. Treat in a stainless steel container with a height of 120cm and an inner diameter of 40cm, which is covered with a 10cm-thick hollow cotton pad for heat preservation, and a 10cm-thick stone is placed at the bottom as a filter layer to collect leachate, and 50kg (wet basis) of the above-mentioned household garbage were put into 6 experiments In the device, wherein 3 experimental devices were added the microbial inoculum prepared in Example 1 according to the ratio of waste mass to 0.1%, and the other 3 experimental devices were sprayed with water with a mass ratio of 0.1% as a blank control, each In the device, a heavy object of 10kg is loaded on...

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Abstract

The invention discloses a microbial agent for garbage composting as well as a preparation method and application thereof. The microbial agent comprises bacillus amyloliquefaciens, candida utilis, lactobacillus acidophilus and rhodopseudomonas palustris and is a liquid bacterial agent or solid bacterial agent. The microbial agent for garbage composting, disclosed by the invention, has the advantages of easiness in production, stable product quality, low cost and strong controllability in a garbage composting process; after the microbial agent is added into the garbage, the content of beneficial efficient microorganisms in the garbage can be increased, the microbial activity is improved, the garbage fermentation process is accelerated, the leaching of water and the increase of the lower calorific value of garbage are promoted, and the incineration efficiency of the garbage is improved; in a region with relatively low winter temperature, the microbial agent can reduce the influence caused by the low-temperature environment change so as to guarantee smooth operation of the garbage incineration according to the technology design; and by adopting the microbial agent, the generation of garbage smell can be reduced, and the working environment is improved.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of domestic garbage, and in particular relates to a microbial agent for fermentation of garbage and its preparation method and application. Background technique [0002] At present, the domestic waste treatment methods in my country mainly include landfill, composting and incineration. Landfilling needs to occupy a large amount of land. Composting is difficult to use due to the low degree of waste classification. Compared with other treatment methods, it has considerable advantages in terms of harm and resource utilization. With the development of urbanization, the land that can be used to bury domestic waste is decreasing, and incineration has become the choice for more and more places to deal with domestic waste. However, my country's municipal solid waste has the characteristics of high moisture content and low garbage calorific value. Most of my country's municipal solid waste has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16B09B3/00C12R1/07C12R1/72C12R1/23C12R1/01
CPCB09B3/00C12N1/16C12N1/20
Inventor 张亮王泽宇孙志刚李玲燕赵志强
Owner QINGDAO GENYUAN BIOLOGICAL TECH GRP