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Ascophyllum Nodosum oligosaccharide preparation method and application of Ascophyllum Nodosum oligosaccharide to medicines for reducing blood sugar

A technology of Ascophyllum nodosum oligosaccharides and Ascophyllum nodosum, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., to achieve the effects of improving enzymatic hydrolysis efficiency, improving sugar metabolism, and convenient operation

Active Publication Date: 2016-08-17
江苏金康神生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are no other research reports or patents for the preparation of Ascophyllum nodosum oligosaccharides with hypoglycemic activity by microwave-assisted step-by-step enzymatic hydrolysis in the present invention.

Method used

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  • Ascophyllum Nodosum oligosaccharide preparation method and application of Ascophyllum Nodosum oligosaccharide to medicines for reducing blood sugar
  • Ascophyllum Nodosum oligosaccharide preparation method and application of Ascophyllum Nodosum oligosaccharide to medicines for reducing blood sugar
  • Ascophyllum Nodosum oligosaccharide preparation method and application of Ascophyllum Nodosum oligosaccharide to medicines for reducing blood sugar

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Embodiment 1

[0027] A preparation method of Ascophyllum nodosum oligosaccharides, the specific steps are as follows: (1) After the Ascophyllum nodosum is dried and crushed, add ethanol with a volume concentration of 95% to the dry powder of the Ascophyllum nodosum and reflux for extraction for 1 hour, the weight ratio of the dry powder of the Ascophyllum nodosum to the ethanol solution It is 1:15, reclaims ethanol after the reflux extraction finishes, and dries to obtain dry defatted Ascophyllum nodosum powder;

[0028] (2) Add distilled water to the Ascophyllum nodosum powder obtained in step (1), wherein the weight ratio of Ascophyllum nodosum powder to distilled water is 1:25, soak for 0.5 h, and irradiate with 500 W microwave for 1 min. After cooling, repeat the irradiation 2 times, after the extraction is completed, filter, repeat the above steps for 2 extractions of the filter residue, mix the obtained filtrate and concentrate to 1 / 4 of the original volume, add 3 times the volume of e...

Embodiment 2

[0043] A preparation method of Ascophyllum nodosum oligosaccharides, the specific steps are as follows: (1) After the Ascophyllum nodosum is dried and crushed, add ethanol with a volume concentration of 95% to the dry powder of the Ascophyllum nodosum and reflux for extraction for 1 hour, the weight ratio of the dry powder of the Ascophyllum nodosum to the ethanol solution It is 1:18, remove ethanol after reflux finishes, dry and obtain dry and defatted Ascophyllum nodosum powder;

[0044] (2) Add distilled water to the Ascophyllum nodosum powder obtained in step (1), wherein the weight ratio of Ascophyllum nodosum powder to distilled water is 1:28, soak for 0.8 h, 700 W microwave irradiation for 2 min, after cooling, repeat the irradiation 2 times, after the extraction is completed, filter, repeat the above steps for 2 extractions of the filter residue, mix the obtained filtrate and concentrate to 1 / 4 of the original volume, add 4 times the volume of ethanol recovered in step ...

Embodiment 3

[0059] A preparation method of Ascophyllum nodosum oligosaccharides, the specific steps are as follows: (1) After the Ascophyllum nodosum dry powder is dried and pulverized, add ethanol with a volume concentration of 95% to the dry powder of the Ascophyllum nodosum and reflux for extraction for 2 hours, the weight ratio of the dry powder of the Ascophyllum nodosum to the ethanol solution Be 1: 20, remove ethanol after reflux finishes, dry and obtain dry defatted Ascophyllum nodosum powder;

[0060] (2) Add distilled water to the Ascophyllum nodosum powder obtained in step (1), wherein the weight ratio of Ascophyllum nodosum powder to distilled water is 1:30, soak for 1 h, 800 W microwave irradiation for 2 min, after cooling, repeat the irradiation 3 times, after the extraction is completed, filter, repeat the above steps for 3 times to extract the filter residue, mix the obtained filtrate and concentrate to 1 / 3 of the original volume, add 4 times the volume of ethanol recovered...

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Abstract

The invention provides an Ascophyllum Nodosum oligosaccharide preparation method and application of Ascophyllum Nodosum oligosaccharide to medicines for reducing blood sugar. The preparation method is characterized by including a technological process of taking degreased, deproteinized and depigmented Ascophyllum Nodosum fucoidin as a raw material, combining microwave-assisted treatment, adding pectinase, cellulose, fucoidanase and sulfatase to subject the fucoidin to enzymolysis, removing the fucoidin not degraded sufficiently and zymoprotein through ethanol precipitation, centrifuging liquid supernatant prior to distillation and concentration, blending the distilled and concentrated liquid supernatant with oligosaccharide generated during polysaccharide extraction prior to sieving by a molecular sieve, and subjecting interception matters to freeze drying so as to obtain the Ascophyllum Nodosum oligosaccharide, wherein ethanol used during preparation is recycled. Through in-vitro and cell tests, the Ascophyllum Nodosum oligosaccharide has a remarkable improvement effect on glycometabolism. The preparation method is an efficient and environment-friendly technological method for preparing the Ascophyllum Nodosum oligosaccharide by microwave-accelerated enzymatic digestion.

Description

technical field [0001] The invention belongs to the production process of functional oligosaccharides by enzymatic hydrolysis, and relates to a method for preparing Ascophyllum nodosum oligosaccharides by a microwave-assisted step-by-step enzymatic hydrolysis method and its application in lowering blood sugar. Background technique [0002] Ascophyllum fucoidan is a water-soluble heteropolysaccharide containing sulfate groups, mainly composed of sulfate-based fucose and a small amount of galactose, mannose, xylose, arabinose, uronic acid, etc., also known as Fucoidan, fucoidan sulfate, the main active ingredient is α-L-fucose-4-sulfate. Ascophyllum oligosaccharides have a variety of biological functions and are widely used in medicine, health products, food and so on. Compared with polysaccharides, oligosaccharides have the advantages of being easily soluble in water, non-antigenic, and have a weaker accumulation effect in the host. However, the large-scale application is g...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/12C12P19/04C12P19/00C07H3/06C07H1/06A61P3/10
CPCC07H1/06C07H3/06C12P19/00C12P19/04C12P19/12C12P19/14
Inventor 赵超杨成凤刘斌陈明军陈玉青
Owner 江苏金康神生物科技有限公司
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