Technological system and technological method for extracting glutathione

A technology of glutathione and extraction process, applied in the field of glutathione preparation, can solve problems such as enzyme liquid residue and subsequent process influence, and achieve the effects of large adsorption capacity, alleviation of environmental protection pressure, and strong sample purification and processing capacity.

Inactive Publication Date: 2016-08-31
JIANGSU CHENGXIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of preparing glutathione by enzymatic method, there will be enzyme liquid residue in the reaction solution, which will have a certain impact on the subsequent process

Method used

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  • Technological system and technological method for extracting glutathione

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Effect test

Embodiment 1

[0030] A glutathione extraction process system, equipment arranged from upstream to downstream, including reactor 1, first material tank 2, first centrifuge 3, filter equipment 4, second material tank 5, resin column 6, The third material tank 7, the fourth material tank 8, the decolorization tank 9, the crystallizing tank 10, the second centrifuge 11, the fifth material tank 12, and also include an organic solvent tank 13, which are respectively located in the tree Refers to the upstream of the column 6, the fourth material tank 8, and the crystallizing tank 10, and is connected by a pipeline;

[0031] Preferably, the organic solvent tank 13 is one of a methanol tank, an ethanol tank, an isopropanol tank, and an acetone tank.

[0032] The extraction process system of glutathione according to claim 1, wherein the filter device 4 is an ultrafiltration device, and the filter membrane pore size of the ultrafiltration device is 8000-10000 Daltons. In this embodiment, the decolori...

Embodiment 2

[0034] A kind of processing method that adopts the extraction process system of above-mentioned glutathione, comprises the steps:

[0035] 1) Preparation of crude GSH: including the following steps:

[0036] 1.1 The material after the reaction of the enzymatic synthesis of glutathione was discharged, the feed liquid was centrifuged to remove insoluble matter, and 55 kg of filtrate was collected;

[0037] 1.2 The obtained filtrate is subjected to ultrafiltration through ultrafiltration equipment, and the ultrafiltration dialysate is collected;

[0038] 1.3 The ultrafiltration dialysate was adjusted to acid and then applied to resin column 6; the diameter-to-height ratio of resin column 6 was 0.05, the pH value of the sample was 2.0, the sample volume was 8mgGSH / ml resin, and the sample flow rate was 0.5bv / h;

[0039] 1.4 Rinse the macroporous resin column with 1bv of purified water at a speed of 0.5bv / h;

[0040] 1.5 Elute and desorb the macroporous resin column with 50% etha...

Embodiment 3

[0049] 1) Preparation of crude GSH: including the following steps:

[0050] 1.1 The material after the reaction of the enzymatic synthesis of glutathione was discharged, the feed liquid was centrifuged to remove insoluble matter, and 55 kg of filtrate was collected;

[0051] 1.2 The obtained filtrate is subjected to ultrafiltration through ultrafiltration equipment, and the ultrafiltration dialysate is collected;

[0052] 1.3 The ultrafiltration dialysate was adjusted to acid and then applied to the resin column; the diameter-height ratio of the resin column was 0.2, the pH value of the sample was 3.5, the sample volume was 20mgGSH / ml resin, and the sample flow rate was 1.5bv / h;

[0053] 1.4 Rinse the macroporous resin column with 3bv of purified water at a speed of 1.5bv / h;

[0054] 1.5 Elute and desorb the macroporous resin column with 95% ethanol at a speed of 2.0bv / h, and collect the ninhydrin chromogenic part of the eluent;

[0055] 1.6 Depressurize the collected eluent...

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Abstract

The invention relates to a technological system for extracting glutathione. The technological system comprises devices which are arrayed from upstream to downstream and include a reaction kettle (1), a first material tank (2), a first centrifugal machine (3), a filter device (4), a second material tank (5), a resin column (6), a third material tank (7), a fourth material tank (8), a decolorization tank (9), a crystallization tank (10), a second centrifugal machine (11) and a fifth material tank (12), and further comprises an organic solvent tank (13). The organic solvent tank (13) is positioned on upstream of the resin column (6), upstream of the fourth material tank (8) and upstream of the crystallization tank (10) and is connected with the resin column (6), the fourth material tank (8) and the crystallization tank (10) by pipelines. The technological system has the advantage that the technological system is applicable to extracting the glutathione.

Description

technical field [0001] The invention relates to a preparation process of glutathione, and belongs to the technical field of medicine and its intermediates. Background technique [0002] Glutathione (glutathione, r-glutamyl cysteingl + glycine, GSH) is a tripeptide containing γ-amide bond and sulfhydryl group, which is composed of glutamic acid, cysteine ​​and glycine. Present in almost every cell of the body. Glutathione can help maintain the normal function of the immune system, and has antioxidant and integrated detoxification effects. The sulfhydryl group on cysteine ​​is its active group (so it is often abbreviated as G-SH), which is easy to interact with certain drugs. (such as paracetamol), toxins (such as free radicals, iodoacetic acid, mustard gas, heavy metals such as lead, mercury, arsenic), etc., have an integrated detoxification effect. [0003] The production methods of glutathione mainly include chemical synthesis, enzymatic conversion and fermentation. At p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/037C07K1/34C07K1/30C07K1/16C07K1/14
CPCC07K5/0215
Inventor 吴锋许刘华祝俊余玉奎任世阔周江刘晓庆
Owner JIANGSU CHENGXIN PHARMA
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