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Composite microbial fertilizer and preparation of same

A compound microbial fertilizer and culture technology, applied in the field of agricultural fertilizers, can solve the problems of unstable product quality and application effect, low overall level of microbial fertilizer industry, and lack of investment in research, etc.

Inactive Publication Date: 2016-08-31
YIWU JINYU INFORMATION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the long-term lack of investment in microbial fertilizer research in China, my country's microbial fertilizer industry still has problems such as low overall level, insufficient technological innovation, and unstable product quality and application effects.

Method used

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  • Composite microbial fertilizer and preparation of same
  • Composite microbial fertilizer and preparation of same
  • Composite microbial fertilizer and preparation of same

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0087] (1) Preparation of bacterial suspension

[0088] Inoculate the Li-2013 single colony grown after streaking on the plate into the seed medium, culture at 40°C for 12 hours at 100 r / min, take 1 mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend with 9 mL of physiological in salt water.

[0089] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis

[0090] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows co...

Embodiment 1

[0139] The weight part array of the compound microbial fertilizer is: 20 parts of Lactobacillus plantarum mixed bacteria agent, 14 parts of Aspergillus mixed culture, 1 part of Bacillus megaterium phosphate solubilizing bacteria agent, 8 parts of Bacillus subtilis bacteria powder, and 0.6 parts of polyglutamic acid.

[0140] The Lactobacillus plantarum mixed bacterial agent consists of Lactobacillus plantarum CGMCC9405 and Lactobacillus plantarum CGMCC11763.

[0141] The weight ratio of the Lactobacillus plantarum mixed bacterial agent is: 6 parts of Lactobacillus plantarum CGMCC9405, 2 parts of Lactobacillus plantarum CGMCC11763.

[0142] Aspergillus mixed culture is composed of Aspergillus awamori culture and Aspergillus niger culture;

[0143] Aspergillus mixed culture parts by weight are 2 parts of Aspergillus awamori culture and 7 parts of Aspergillus niger culture.

[0144] The polyglutamic acid is γ-polyglutamic acid.

[0145] The preparation method of described compo...

Embodiment 2

[0175] The technical problem to be solved by the present invention is to provide a compound microbial fertilizer.

[0176] The weight part array of the compound microbial fertilizer is: 30 parts of Lactobacillus plantarum mixed bacteria agent, 16 parts of Aspergillus mixed culture, 1 part of Bacillus megaterium phosphate solubilizing bacteria agent, 10 parts of Bacillus subtilis bacteria powder, 0.5 parts of polyglutamic acid.

[0177] The mixed bacterial agent of Lactobacillus plantarum is composed of Lactobacillus plantarum CGMCC9405 and Lactobacillus plantarum CGMCC11763.

[0178] The parts by weight of Lactobacillus plantarum mixed bacterial agent are 8 parts of Lactobacillus plantarum CGMCC9405 and 2 parts of Lactobacillus plantarum CGMCC11763.

[0179] Aspergillus mixed culture is composed of Aspergillus awamori culture and Aspergillus niger culture;

[0180] The parts by weight of the mixed culture of Aspergillus constitute 1 part of Aspergillus awamori culture and 7 p...

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Abstract

The invention discloses a composite microbial fertilizer comprising, by weight, 15-30 parts of a lactobacillus plantarum mixed inoculant, 10-16 parts of an aspergillus mixed culture substance, 1-3 parts of a bacillus megaterium var phosphaticum inoculant, 5-10 parts of powder of bacillus subtilis and 0.5-1 part of polyglutamic acid. The polyglutamic acid on surfaces of fertilizer granules can absorb moisture during actual usage process to form hydrogel, thereby providing a moisture environment for rapid growth and reproduction of the fertilizer inside the granules and meanwhile achieving a slow-release effect of fertility of bio-bacteria.

Description

technical field [0001] The invention relates to a micro-ecological fertilizer and belongs to the technical field of agricultural fertilizers. Background technique [0002] Microbial fertilizer is a living fertilizer, and its function is mainly completed by the life activities of a large number of beneficial microorganisms it contains. Only when the life activities of these beneficial microorganisms are completed. Only when these beneficial microorganisms are vigorously multiplying and metabolizing, can material transformation and beneficial metabolites be continuously formed. Therefore, the types of beneficial microorganisms in microbial fertilizers and whether their life activities are vigorous are the basis of their effectiveness, unlike other fertilizers that are based on the form and amount of major elements such as nitrogen, phosphorus, and potassium. Because microbial fertilizers are living preparations, their fertilizer efficiency is closely related to the number, s...

Claims

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Application Information

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IPC IPC(8): C09K17/14C09K101/00
CPCC09K17/14C09K2101/00
Inventor 李秉京
Owner YIWU JINYU INFORMATION TECH CO LTD
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