Efficient and stable fruit tree RNA extraction method

Abstract

The invention relates to an efficient and stable fruit tree RNA extraction method, in particular to an improved method of extracting perennial herbal, woody and liana fruit tree RNA through a CTAB method. Tests show that the method can be used in tissue of different fruit tree species like tissue culture seedlings and field seedlings of strawberry, apple and grape, and extracted RNA of the tissue of the fruit tress is high in purity and good in effect. Nucleic acid analysis finds that by using the method, secondary metabolites like phenolics, protein, polysaccharide and micromolecule salt ions unique in fruit tree tissue cells can be removed effectively, and a good option is provided for RNA extraction and preparation in molecular biology experiments.

Description

technical field [0001] The invention relates to an efficient and stable method for extracting fruit tree RNA, belonging to the technical field of molecular biology. Background technique [0002] With the gradual enrichment of fruit tree genome data, more and more fruit tree species genome information has been annotated. Therefore, the development of fruit tree molecular biology is accepted by more and more fruit tree breeders for the purpose of mining functional genes and serving fruit tree breeding through genetic engineering and other means. [0003] As a perennial woody or herbaceous plant, fruit trees are rich in polyphenols, polysaccharides and other fruit tree-specific secondary metabolites due to their perennial characteristics, which brings a lot of trouble to the extraction and purification of fruit tree RNA. [0004] Biological kits are quick and easy for molecular biology research, but there are no kits for RNA extraction of fruit tree species in the market today...

AI Technical Summary

Problems solved by technology

[0005] Commonly used traditional RNA extraction methods include Trizol method, phenol method, lithium chloride precipitation method, guanidine isothiocyanate method, and SDS / phenol extraction method. The above methods are expensive, time-consuming and labor-intensive, and the fruit tree RNA obtained less output

In addition, through experiments, the applicant found that the Trizol method is mostly suitable for herbaceous plants, but not for RNA extraction of fruit trees and woody plant materials.

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