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Method for separating and extracting maize yellow pigment and zein from maize protein powder by using membrane separation technology

A technology for zein and corn gluten meal, which is applied to the preparation methods, chemical instruments and methods of plant zein and peptides, etc., can solve the problems such as the failure of separation and utilization of yellow pigment, and achieves the improvement of added value and Economic benefits, improved product quality, the effect of continuous extraction

Inactive Publication Date: 2016-09-21
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method obtains high-quality zein, the yellow pigment in corn gluten powder cannot be separated and utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Crush corn gluten powder and pass through a 80-mesh sieve; weigh 50 g of corn gluten powder under the sieve, mix it with 500 mL of 80% ethanol aqueous solution (solid-to-liquid ratio 1:10), and then extract it ultrasonically under dark conditions (ultrasonic temperature 30°C, ultrasonic power 300W, ultrasonic frequency 60KHz, ultrasonic time 120min); after ultrasonication, centrifuge (speed 8000 rpm, time 30min) to obtain the supernatant; The speed is 165rpm, the vacuum degree is 0.05MPa), and the zeaxanthin solution and the zein solution are respectively obtained.

[0034] Concentrate the zeaxanthin solution in vacuum (vacuum concentration temperature is 40°C, vacuum pressure is 0.05MPa, concentration time is 60 min.) to obtain a red oily liquid, which is the zeaxanthin product. After testing, the content of zeaxanthin in the product is 0.278mg / g, and the extraction rate is 91.45%.

[0035] The zein solution was washed with water, precipitated, and vacuum freeze-dried...

Embodiment 2

[0037]Crush the corn gluten powder and pass through a 60 sieve. Weigh 50g of corn gluten powder under the sieve, mix 400mL with 70% by volume ethanol aqueous solution (solid-to-liquid ratio is 1:8), and then extract ultrasonically under dark conditions (ultrasonic temperature 40°C, ultrasonic power 300W, ultrasonic frequency 50 KHz, ultrasonic 60min.). After sonication, centrifuge (centrifugation speed 5000 rpm, centrifugation time 20min) to obtain the supernatant. Then the supernatant was subjected to membrane separation (the pore size of the membrane was 5kDa, the membrane passing rate was 137rpm, and the vacuum degree was 0.04MPa.) to obtain zeaxanthin solution and zein solution respectively.

[0038] Concentrate the zeaxanthin solution in vacuum (the temperature is 35°C, the vacuum pressure is 0.06MPa, the concentration time is 45min.), and the obtained red oily liquid is the zeaxanthin product. After testing, the content of zeaxanthin in the product is 0.264mg / g, and th...

Embodiment 3

[0041] Crush corn gluten powder and pass through a 100-mesh sieve; weigh 50 g of corn gluten powder under the sieve, mix it with 750 mL of 85% ethanol solution (solid-to-liquid ratio 1:15), and perform ultrasonic extraction under dark conditions (ultrasonic temperature 45°C, power 300W, ultrasonic frequency 60 KHz, ultrasonic 240min.). After ultrasonication, centrifuge (centrifugation speed 10000 rpm, centrifugation time 10min) to obtain the supernatant; then perform membrane separation on the supernatant (the membrane pore size is 5kDa, the membrane passing rate is 183rpm, and the vacuum degree is 0.06MPa.), respectively, to obtain Zeaxanthin solution and Zein solution.

[0042] Concentrate the zeaxanthin solution in vacuum (the vacuum concentration temperature is 45°C, the vacuum pressure is 0.06MPa, and the concentration time is 120 min.) into a red oily liquid, which is the zeaxanthin product. After testing, the content of zeaxanthin in the product is 0.282mg / g, and the e...

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Abstract

The invention provides a method for separating and extracting a maize yellow pigment and zein from maize protein powder by using a membrane separation technology. The method comprises the steps: crushing the maize protein powder, then, mixing the crushed maize protein powder with an aqueous solution of ethanol, and carrying out ultrasonic extraction under shaded conditions; carrying out centrifugation after the ultrasonic extraction is completed, so as to obtain supernatant; carrying out membrane separation on the supernatant, so as to obtain a maize yellow pigment solution and a zein solution; subjecting the maize yellow pigment solution to vacuum concentration so as to obtain red oily liquid, i.e., the maize yellow pigment product; subjecting the zein solution to water washing, precipitating and drying, so as to obtain white solids, i.e., the zein. According to the method, the continuous extraction of the maize yellow pigment and the zein is achieved, and meanwhile, the product quality of the maize yellow pigment and the zein is improved. Compared with the existing technologies which are used for carrying out single processing on the maize protein powder, the method has the advantages that the yield of products, i.e., the maize yellow pigment and the zein is increased, the purity of the products is improved, and thus, the added value and economic benefit of the maize protein powder are improved.

Description

technical field [0001] The invention relates to a method for separating and extracting corn yellow pigment and zein from corn gluten powder by using membrane separation technology, and belongs to the technical field of deep processing of corn gluten powder. Background technique [0002] Deep processing of agricultural products and increasing the added value of agricultural products is a long-term national policy of my country's agricultural development. Corn is the main food crop in my country, and its annual output is second only to the United States, ranking second in the world. Corn starch processing has made a great contribution to my country's starch industry. Corn gluten meal is the main by-product in the process of corn starch processing. In order to increase its added value, zeaxanthin and gliadin have received attention. [0003] The protein content of corn gluten powder is about 40%~60%, but due to the lack of essential amino acids such as lysine and tryptophan, i...

Claims

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Application Information

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IPC IPC(8): C07K14/425C07K1/34C07C403/24
CPCC07K14/425C07C403/24
Inventor 刘红岳莹邵士俊梁卿徐健
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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