Paenibacillus mucilaginosus N6 and application thereof

A technology of Paenibacillus and Bacillus, applied in Paenibacillus colioids N6 and its application fields, can solve the problems of low yield, prone to lodging, poor stress resistance, etc., achieve significant increment, promote growth and development, and promote coloring rate Effect

Inactive Publication Date: 2016-09-21
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the case of potassium deficiency, plants have low yield, poor stress resistance, and are prone to lodging
[0003] The potassium needed by crops is still mainly provided by chemical potassium fertilizers, but the distribution of global potassium mineral resources is extremely uneven, and the production of chemical potassium fertilizers has a high concentration in terms of regions and manufacturers. The production capacity of manufacturing enterprises is relatively small, resulting in 70% of my country's current chemical potash fertilizers relying on imports, and the supply of potash fertilizers is heavily dependent on the international market. The domestic price of potash fertilizers has long been subject to the international market, lacking bargaining power

Method used

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  • Paenibacillus mucilaginosus N6 and application thereof
  • Paenibacillus mucilaginosus N6 and application thereof
  • Paenibacillus mucilaginosus N6 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Cultivation of Paenibacillus colioids N6.

[0025] Silicate bacteria medium: sucrose 5g, Na2HPO4 2g, MgSO4 7H2O 0.5g, FeCl3 0.005g, CaCO3 0.1g, soil minerals * 1.0g, agar 15~20g, water 1000ml, pH 70~7.5

[0026] Soil minerals: remove organic residues from the soil, add 6N HCl to boil for half an hour, filter, rinse with distilled water until there is no reaction of chloride ions. Or use 1.0g of potassium feldspar powder.

[0027] The medium is sterilized, and when the temperature drops to about 60°C, add 1g of boiled soil minerals or potassium feldspar powder washed with hydrochloric acid, mix well, and pour onto a plate. Paenibacillus coli N6 can grow on silicate solid medium.

Embodiment 2

[0028] Embodiment 2: Determination of Paenibacillus colioids N6 liquid potassium decomposing ability

[0029] Liquid potassium solution test medium: sucrose 10.0g, Na 2 HPO 4 2.0g, MgSO 4 ·7H 2 O 0.5g, (NH 4 ) 2 SO 4 1.0g, NaCl 0.1g, CaCO 3 1.0g, yeast extract 0.5g, potassium feldspar powder 10.0g, water 1000mL, pH 7.2.

[0030] Grind potassium feldspar, pass through a 100-mesh sieve, wash with water to remove water-soluble potassium, and dry in the shade. 50 mL of the above-mentioned culture medium was divided into 250 mL Erlenmeyer flasks, 1.0 g of soil minerals (100 mesh) were added, sterilized at 121 °C for 30 min, and 2 colonies with a diameter close to 1 cm were inserted. The control was not inoculated, and the experiment was repeated 5 times. After shaking culture at 28°C for 5 days, the increase of available potassium was analyzed by flame photometer, and the results were as follows: figure 2 shown.

[0031] from figure 2 As a result, it can be seen that...

Embodiment 3

[0032] Example 3: Paenibacillus colioids N6 capsule staining

[0033] Materials: Paenibacillus coli N6, capsule staining solution, slide glass, pure methanol, black carbon ink, sterile water.

[0034] Steps: Put a drop of sterile water on the glass slide, take a little Paenibacillus colioids N6 cultured for 72 hours to make a suspension in water, take a drop of prepared black carbon ink and mix it with the bacteria suspension, and take another piece The glass slide is used as a pusher, after contacting the flat edge of one end of the pusher with the suspension at an angle of 30 degrees, the suspension is pushed forward to form a uniform and thin layer, and air-dried. Fix with pure methanol for 1 minute, add lycopene drop on the smear, wash away the residual methanol, and stain for 30 seconds, rinse with a thin stream of water, blot dry and check with oil microscope, the background is black, the capsule is colorless, and the cells are red ,like image 3 shown.

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Abstract

The invention discloses paenibacillus mucilaginosus N6 and application thereof. The classified name of the strain is the paenibacillus mucilaginosus N6 which is collected in China Center for Type Culture Collection, and the collection number is CCTCC NO: M2015548. The paenibacillus mucilaginosus N6 can be grown under a plate cultivation condition of silicate bacteria culture media, and can dissociate potassium from minerals. Under the liquid oscillation culture condition, rapidly-available-potassium increment is compared with a contrast by a flame spectrophotometer and is found great. Bowl experiments show that growth and development of corn can be promoted obviously after the corn is inoculated with the paenibacillus mucilaginosus N6; experiments in orchards show that tinctorial yield of apples can be promoted obviously through application of N6 fermentation liquor. By the paenibacillus mucilaginosus N6, excellent strain resources are provided for improvement in corn growth and apple quality.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers, and in particular relates to Paenibacillus colioids N6 and an application thereof. Background technique [0002] Plant growth requires a large amount of chemical elements such as nitrogen, phosphorus, and potassium. Among them, the importance of potassium is often ignored by farmers: For decades, there has been a "heavy application of nitrogen and phosphorus fertilizers, less or no application of fertilizers" in agricultural production in my country. Potassium" phenomenon. In fact, potassium is not only necessary for the growth and development of some important food crops (for example, the demand for potassium fertilizer in rice is even higher than that for nitrogen and phosphorus fertilizers); it is also required for a series of economic crops, such as cotton, tea, tobacco, watermelon, banana, etc. a large number of elements. In the case of potassium deficiency, plants have low yi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P21/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 樊奔夏永仙杨磊陈晟李昱龙
Owner NANJING FORESTRY UNIV
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