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Circulating free DNA vacuum collection tube

A collection tube and vacuum technology, applied in the field of blood collection tubes, to achieve the effects of improving work efficiency, reducing storage conditions, and reducing labor load

Inactive Publication Date: 2016-10-05
付士明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defect that special conditions must be given to blood samples in the detection of existing circulating free DNA, and to invent a circulating free DNA vacuum collection tube that can collect and preserve blood samples at normal temperature

Method used

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  • Circulating free DNA vacuum collection tube
  • Circulating free DNA vacuum collection tube
  • Circulating free DNA vacuum collection tube

Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0036] The original tube is a PET tube with a capacity of 7ml, the rubber stopper is a bromobutyl rubber stopper, 0.8g of separation glue is added to the bottom of the tube, and then 0.1ml of additives are added. The additives contain 2.2mg EDTAK3, 10mg imidazolidinyl urea, 10mg glycine, 2mg Sodium fluoride, 2mgTAT, vacuum preset volume is 5ml, 5ml of venous blood is drawn and centrifuged after vacuum plugging, the centrifugal force is 1600g, and the centrifugation time is 10 minutes, respectively on the day of blood drawing, 3 days after blood drawing, and after blood drawing On the 7th and 14th day after the blood was drawn, the blood cell rupture and white blood cell death (trypan blue staining) of the whole blood before and after centrifugation were observed under a microscope, as well as the remaining blood cells in the plasma and the fragments of the broken blood cells. The results are as follows:

[0037]

[0038] The above results prove that there is no blood cell ru...

no. 2 example

[0043] The original tube is a PET tube with a capacity of 10ml, and the rubber stopper is a bromobutyl rubber stopper. Add 1.2g of separation glue to the bottom of the tube, and then add 0.2ml of additives. The additives contain 4.4mg of EDTAK3, 20mg of diazolidinyl urea, and 20mg of glutamic acid. Amide, 2mg glyceraldehyde, 4mgTAT, vacuum preset volume is 10ml, 10ml of venous blood is drawn and centrifuged after vacuuming and plugging, the centrifugation condition is centrifugal force 1600g, centrifugation time is 10 minutes, on the day of blood drawing, 3 days after blood drawing, respectively. On the 7th day and 14th day after blood drawing, the blood cell rupture and white blood cell death (trypan blue staining) of the whole blood before and after centrifugation were observed under a microscope, as well as the residual blood cells in the plasma and the fragments of the broken blood cells. The results are as follows:

[0044]

[0045] The above results prove that there is...

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Abstract

The invention discloses a blood collection tube, and particularly relates to a circulating free DNA vacuum collection tube which can collect and preserve a blood sample under the room temperature condition. The circulating free DNA vacuum collection tube is composed of an original tube and a rubber plug; an additive and blood separating gel are contained in the original tube, 0.6-1.2 g of the blood separating gel 4 is added into the bottom of the original tube 1 with the volume of 5 ml, then 0.05-6 ml of the mixed additive is added, the original tube is vacummized, the rubber plug is connected to a tube opening of the original tube in a sealing mode, and a tag is stuck to the outer surface of the original tube; a protective cap is arranged outside the rubber plug; the rubber plug can be replaced with aluminum plastic film. According to the circulating free DNA vacuum collection tube, the collected blood sample can keep the initial state for 14 days under the room temperature condition, and a detection result is not influenced; the centrifuged blood separating gel can separate visible components in the blood from plasma, therefore, the influence of leukocytes, erythrocytes and thrombocytes on free circulating DNA in the preservation process is effectively prevented, and the separated plasma still can keep the initial state for 14 days or above; the storage and transportation conditions after blood sample collection are reduced, and the working efficiency is improved.

Description

technical field [0001] The invention relates to a blood collection tube, in particular to a circulating free DNA vacuum collection tube which can collect and preserve blood samples under normal temperature conditions. Background technique [0002] Free circulating DNA is mainly composed of single-strand or double-strand or a mixture of single and double strands, and exists in the form of DNA-protein complexes or free DNA. Cell-free circulating DNA has been detected in samples such as peripheral plasma, serum, other body fluids, bile, and feces. The detection of free circulating DNA can be applied to the diagnosis of trauma, stroke, diabetes and autoimmune diseases, so the extraction and detection of free circulating DNA will lay the foundation for its application in the field of medical diagnosis, especially the detection of gene molecular biology. [0003] At present, the detection of free circulating DNA is mainly used in the fields of prenatal diagnosis, early tumor diag...

Claims

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Application Information

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IPC IPC(8): C12M1/24C12N15/10
Inventor 付士明
Owner 付士明
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