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Method for extracting anthocyanin from nitraria tangutorum peel residues

A technology of Nitraria rind and anthocyanins, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of long extraction time and low extraction efficiency, and achieve improved dissolution rate, improved extraction rate, and improved Yield effect

Inactive Publication Date: 2016-10-12
QINGHAI TSINGHUA BIOTRY BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, water extraction and organic solvent extraction have the disadvantages of long extraction time and low extraction efficiency.
At present, the research and extraction of anthocyanins are mainly concentrated in fruits such as blueberries, grapes, red currants, black currants, strawberries, apples, cherries, etc., and there are almost no studies on extracting anthocyanins from Nitraria peel residues.

Method used

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  • Method for extracting anthocyanin from nitraria tangutorum peel residues
  • Method for extracting anthocyanin from nitraria tangutorum peel residues

Examples

Experimental program
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Effect test

Embodiment 1

[0042] This embodiment provides a method for extracting anthocyanins from Nitraria rind residue, comprising the following steps:

[0043] (1) Pretreatment step: freeze the Nitraria rind residue at -40°C for 5 hours, and then dry it to constant weight by vacuum drying under reduced pressure. Wherein the parameters of the decompression vacuum drying method are: the degree of vacuum is 0.05MPa, and the temperature is 10°C. Then the dried Nitraria pericarp residue was crushed to 80 mesh to obtain Nitraria pericarp residue powder.

[0044] (2) Enzymolysis step: mix Nitraria pericarp residue powder with cellulase for enzymolysis treatment, the amount of cellulase added is 0.2% of the weight of Nitraria pericarp residue powder, the enzymolysis temperature is 40°C, and the enzymolysis time for 60 minutes, followed by filtration to obtain Nitraria rind residue enzymatic hydrolysis solution.

[0045] (3) Extraction step: after mixing the enzymatic hydrolysis solution of Nitraria peric...

Embodiment 2

[0048] This embodiment provides a method for extracting anthocyanins from Nitraria rind residue, comprising the following steps:

[0049] (1) Pretreatment step: freeze the residue of Nitraria rind at -30°C for 7 hours, and then dry it to constant weight by vacuum drying under reduced pressure. Wherein the parameters of the decompression vacuum drying method are: the degree of vacuum is 0.2 MPa, and the temperature is 2°C. Then the dried Nitraria pericarp residue was crushed to 70 mesh to obtain Nitraria pericarp residue powder.

[0050] (2) Enzymolysis step: the Nitraria peel residue powder is mixed with cellulase and xylanase for enzymolysis treatment. Wherein the added amount of cellulase is 0.04% of the weight of Nitraria pericarp residue powder, and the added amount of xylanase is 0.12% of the weight of Nitraria pericarp residue powder. The enzymolysis temperature is 42° C., and the enzymolysis time is 50 minutes, followed by filtration to obtain the enzymolysis solution...

Embodiment 3

[0054] This embodiment provides a method for extracting anthocyanins from Nitraria rind residue, comprising the following steps:

[0055] (1) Pretreatment step: freeze the residue of Nitraria rind at -25°C for 7.5 hours, and then dry it to constant weight by vacuum drying under reduced pressure. Wherein the parameters of the decompression vacuum drying method are: the degree of vacuum is 0.15MPa, and the temperature is 6°C. Then crush the dried Nitraria pericarp residue to 60 mesh to obtain Nitraria pericarp residue powder.

[0056] (2) Enzymolysis step: the Nitraria peel residue powder is mixed with cellulase and xylanase for enzymolysis treatment. Wherein the added amount of cellulase is 0.06% of the weight of Nitraria pericarp residue powder, and the added amount of xylanase is 0.1% of the weight of Nitraria pericarp residue powder. The enzymolysis temperature is 42.5° C., and the enzymolysis time is 45 minutes, followed by filtration to obtain the enzymolysis solution of...

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Abstract

The invention provides a method for extracting anthocyanin from nitraria tangutorum peel residues. The method comprises the following steps of pretreatment, wherein the nitraria tangutorum peel residues are subjected to vacuum drying and then smashed to obtain nitraria tangutorum peel residue powder; enzymolysis, wherein the nitraria tangutorum peel residue powder and a plant cell wall degrading enzyme are mixed to be treated; extraction, wherein nitraria tangutorum peel residue enzymatic liquid and an organic solvent are mixed, and the mixture is extracted to obtain a crude nitraria tangutorum peel residue extract; purification, wherein the crude nitraria tangutorum peel residue extract is purified by adopting an adsorption chromatography method to obtain the anthocyanin. According to the method, the nitraria tangutorum peel residues are frozen at low temperature, dried through a vacuum drying method and smashed, it is effectively guaranteed that the structure of part of the anthocyanin, being sensitive to temperature, in the nitraria tangutorum peel residues is not destroyed, and therefore the finally obtained anthocyanin is more in compound variety and high in effective ingredient content. The separation efficiency of the method is high, and the anthocyanin prepared through the method is large in mass and high in purity.

Description

technical field [0001] The invention relates to the field of natural products, in particular to a method for extracting anthocyanins from Nitraria rind residue. Background technique [0002] Anthocyanin is a water-soluble pigment that belongs to flavonoids and mostly exists in the form of glycosides, also known as anthocyanins. As a natural food coloring, anthocyanins are safe, non-toxic, rich in resources, and have significant pharmacological effects. They have great application potential in food, cosmetics, and medicine. [0003] Nitraria L. is a small deciduous shrub of the genus Nitraria L. in the family Zygophyllaceae. It often grows on the ground, plant height 30-50cm, multi-branched, a few branches upright, bark light yellow, branchlets off-white, tips thorny, branches without thorns or few thorns. Nitraria has strong adaptability, tolerance to drought, saline-alkali preference, cold resistance, wind resistance, high temperature resistance, and barrenness. It is a t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C09B61/00C09B67/54
CPCC07D311/62C09B61/00C09B67/0096
Inventor 王欢安月玲王锦凤米世文龚晨于雪
Owner QINGHAI TSINGHUA BIOTRY BIO TECH
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