Method for continuously extracting maize yellow pigment and zein from maize protein meal

A technology for zein and corn gluten meal, which is applied to the preparation methods of peptides, chemical instruments and methods, natural dyes, etc., can solve the problems of residual toxic and harmful chemicals, low quality, and chroma that cannot meet application requirements. , to achieve the effect of improving protein content and product quality, improving purity and quality, and improving economic benefits

Active Publication Date: 2016-10-12
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses petroleum ether for extraction, which will cause toxic and harmful chemicals to remain in zein and zeaxanthin, and the obtained zein has a yellow color and low quality, and cannot reach the international market. requirements
In the above-mentioned methods, since the pigment in zein cannot be completely removed, the color of the extracted zein is yellowish, the product quality is not ideal, and the chroma does not meet the application requirements of the international market, which greatly reduces the utilization rate. value, making the application of zein in medicine and food limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Crush the corn gluten powder and pass through a 80-mesh sieve; weigh 100g of the corn gluten powder under the sieve, mix it with 400mL of n-hexane (solid-to-liquid ratio 1:4), and ultrasonically treat it in the dark, at a temperature of 5°C, at a power of 300W, and at a frequency of 60KHz 60min; filter to obtain defatted corn gluten powder; then mix defatted corn gluten powder with 900mL of absolute ethanol (solid-to-liquid ratio 1:9), and avoid light for ultrasonic extraction (temperature 5°C, power 300W, frequency 60KHz, ultrasonic treatment 60min.); Centrifuge (speed 8000rpm, time 20min) to obtain supernatant and filter residue.

[0042] The supernatant was concentrated at a temperature of 40°C and a vacuum pressure of 0.06 MPa for 60 min to obtain a red oily liquid, namely zeaxanthin. After testing, the content of zeaxanthin in the pigment was 0.265mg / g, and the extraction rate was 87.17%.

[0043] Weigh 50g of filter residue, add 1000mL water to mix, adjust pH=5.5...

Embodiment 2

[0045] Crush the corn gluten powder and pass through a 60-mesh sieve; weigh 100g of the corn gluten powder under the sieve, mix it with 500ml of n-hexane (solid-to-liquid ratio 1:5), and perform ultrasonic treatment in the dark (ultrasonic temperature 40°C, power 300W, ultrasonic Frequency is 60KHZ, ultrasonic 45min) to remove fat, filter to obtain defatted corn gluten powder. Then mix the defatted corn gluten powder with 1500mL of absolute ethanol (solid-to-liquid ratio 1:15), avoid light and ultrasonically extract (ultrasonic temperature 40°C, power 300W, ultrasonic frequency 60KHZ, ultrasonic 45min); centrifuge (speed 5000rpm, Time 25min) to obtain supernatant and filter residue.

[0046] The supernatant was concentrated at a temperature of 45°C and a vacuum pressure of 0.06 MPa for 180 min to obtain a red oily liquid, namely zeaxanthin. After testing, the content of zeaxanthin in the yellow pigment was 0.258mg / g, and the extraction rate was 86.58%.

[0047] Weigh 50g of ...

Embodiment 3

[0049] Crush the corn gluten powder and pass through a 100-mesh sieve; weigh 200g of the corn gluten powder under the sieve, mix it with 1200ml of n-hexane (solid-to-liquid ratio 1:6), and perform ultrasonic treatment in the dark (ultrasonic temperature 30°C, power 200W, ultrasonic Frequency is 50KHz, ultrasonic 120min.) to remove fat, filter to obtain defatted corn gluten powder. Then mix defatted corn gluten powder with 2000mL of absolute ethanol (solid-to-liquid ratio 1:10), avoid light and ultrasonically extract (ultrasonic temperature 30°C, power 200W, ultrasonic frequency 50KHz, ultrasonic 120min.); centrifuge (speed 10000rpm , time 15min) to obtain supernatant and filter residue.

[0050] The supernatant was concentrated at a temperature of 40°C and a vacuum pressure of 0.06 MPa for 70 minutes to obtain a red oily liquid, namely zeaxanthin. After testing, the content of zeaxanthin in the pigment was 0.289mg / g, and the extraction rate was 93.23%.

[0051] Weigh 50g of ...

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PUM

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Abstract

The invention relates to a method for continuously extracting and separating maize yellow pigment and zein from maize protein meal with high yield and high quality, and belongs to the technical field of deep processing of the maize protein meal. The method comprises the following steps: carrying out degreasing with n-hexane, carrying out extraction in an opaque background with ultrasonic waves, and carrying out centrifugation to obtain supernate and filter residue; conducting vacuum concentration on the supernate to obtain red oily maize yellow pigment; conducting enzymolysis and starch removal treatment on the filter residue with alpha-amylase and beta-glycosidase, and carrying out microwave extraction and centrifugation to obtain a zein extract; adjusting the pH value of the zein extract to a protein isoelectric point, carrying out washing with water and precipitation to obtain wet zein powder, and carrying out vacuum freeze-drying to obtain a white zein product. The content of the corn yellow pigment in the product is 0.25mg / g to 0.29mg / g; the extraction rate is 86% to 94%; the protein content of the zein product is 90% to 93% by mass; the yield of zein is 39% to 46%.

Description

technical field [0001] The invention relates to a method for continuously extracting and separating zeaxanthin and zein from corn gluten powder, and belongs to the technical field of deep processing of corn gluten powder. Background technique [0002] Corn is one of the three major food crops in the world and is recognized as a golden food. The current global production has exceeded 700 million tons. my country has a wide range of corn planting areas and areas. According to data released by the National Bureau of Statistics, in 2015, the national corn planting area was 38.2 million hectares (about 570 million mu), and the output could reach 229 million tons. Corn is widely used in the three major directions of livestock and poultry feed, food and intensive processing. Corn gluten meal is a by-product produced in the process of wet processing corn starch, with an annual output of about 2.2 million tons. Most corn starch manufacturers sell corn gluten meal as cheap feed, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C07K1/30C07K1/14
CPCC07K1/14C07K1/30C09B61/00
Inventor 刘红岳莹邵士俊梁卿徐健
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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