A method for detecting diphenols in smokers saliva
A technology for smokers and diphenols, applied in the field of analytical chemistry, can solve the problems of low content of diphenols, difficult to collect and store, high requirements, etc., and achieves the effects of simple steps, improved analysis accuracy, and reduced pollution
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0018] Collect 1 mL of saliva from smokers with a saliva collection tube, add 1 mL of methanol extraction solution and mix well, add 3 g of water remover anhydrous magnesium sulfate, take 1 mL of extraction solution into a chromatographic bottle, quickly seal it, and perform gas chromatography- Mass spectrometry analysis, quantitative according to component peak area.
[0019] The gas chromatography conditions are as follows: the chromatographic column is a DB-5MS column, the length is 30 m × the inner diameter is 0.25 mm × the film thickness is 0.25 µm; the carrier gas is helium (He); the inlet temperature is 160 ºC; constant flow mode, column flow 1.0 mL / min, split ratio 5:1; programmed temperature rise to 50 ºC, hold for 5 min, then increase to 130 ºC at a rate of 2 ºC / min, hold for 10 min.
[0020] The mass spectrometry conditions are: ionization mode is electron bombardment source (EI), ion source temperature is 200 ºC, ionization energy is 60eV, quadrupole temperature is...
Embodiment 2
[0023] Collect 5 mL of saliva from smokers with a saliva collection tube, add 5 mL of methanol extraction solution and mix well, add 5 g of water remover anhydrous magnesium sulfate, take 2 mL of extraction solution into a chromatographic bottle, seal it quickly, and perform gas chromatography- Mass spectrometry analysis, quantitative according to component peak area.
[0024] The gas chromatography conditions are as follows: the chromatographic column is a DB-5MS column, the length is 30 m × the inner diameter is 0.25 mm × the film thickness is 0.25 µm; the carrier gas is helium (He); the inlet temperature is 170 ºC; constant flow mode, column flow 1.5 mL / min, split ratio 10:1; programmed temperature rise to 60 ºC, hold for 5 min, then increase temperature to 150 ºC at a rate of 5 ºC / min, hold for 20 min.
[0025] The mass spectrometry conditions are: ionization mode is electron bombardment source (EI), ion source temperature is 220 ºC, ionization energy is 80eV, quadrupole t...
Embodiment 3
[0028] Collect 2 mL of saliva from smokers with a saliva collection tube, add 2 mL of methanol extraction solution and mix well, add 4 g of water remover anhydrous magnesium sulfate, take 1.5 mL of extraction solution into a chromatographic bottle, seal it quickly, and perform gas chromatography- Mass spectrometry analysis, quantitative according to component peak area.
[0029] The gas chromatography conditions are as follows: the chromatographic column is a DB-5MS column, the length is 30 m × the inner diameter is 0.25 mm × the film thickness is 0.25 µm; the carrier gas is helium (He); the inlet temperature is 165 ºC; constant flow mode, column flow 1.2 mL / min, split ratio 8:1; program temperature rise to 55 ºC, hold for 5 min, then increase temperature to 140 ºC at a rate of 3 ºC / min, hold for 15 min.
[0030] The mass spectrometry conditions are: ionization mode is electron bombardment source (EI), ion source temperature is 210 ºC, ionization energy is 70eV, quadrupole tem...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More