Multi-connected detection reagent card adopting immunochromatography and used for respiratory pathogens

A detection reagent and immunochromatography technology, which is applied in the field of medical inspection, can solve the problems of long time-consuming joint inspection of various pathogens, lack of series, and high requirements for operators' professional skills, so as to save detection time and cost, reduce The error of the supervisor's judgment and the effect of low requirements for professional skills

Inactive Publication Date: 2016-10-26
ANBIO XIAMEN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are various detection methods for respiratory pathogens, it is still very difficult to confirm the pathogen of infection
Bacterial pathogen isolation and virus culture tissue cell culture method used to be used as the "gold standard", but it is difficult to achieve due to the disadvantages of complicated operation, long culture time, technical difficulty and low positive rate, and has been adopted in the past 10 years. Molecular approach replaced by
With the rapid development of rapid diagnostic technology, molecular detection technology is becoming more and more perfect, and has been used as a new standard for the detection of respiratory pathogens, but it cannot be popularized due to its high requirements on laboratory conditions, and there are few commercial products, and no series have been formed. , most pathogens are not yet detectable
In recent years, the development of immune technology, especially the wide application of immune labeling technology, such as direct and indirect immunofluorescence, anti-alkaline phosphatase bridge enzyme labeling method and o

Method used

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  • Multi-connected detection reagent card adopting immunochromatography and used for respiratory pathogens
  • Multi-connected detection reagent card adopting immunochromatography and used for respiratory pathogens
  • Multi-connected detection reagent card adopting immunochromatography and used for respiratory pathogens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] This embodiment provides the assembly method of the joint detection test strip of influenza A virus and influenza B virus, such as figure 1 Shown:

[0062] The nitrocellulose membrane 101 connected with absorbent paper is divided into two parts: absorbent paper 102 and nitrocellulose membrane 103, and the sample pad 104 is combined with one end of the nitrocellulose membrane 103 to obtain influenza A virus and influenza B virus Joint detection test strip 106.

[0063] The side of the sample pad 104 and the absorbent paper-attached nitrocellulose membrane 101 facing away from the detection line is also provided with a coating film and a backing.

[0064] Wherein the nitrocellulose membrane 103 is provided with a first detection line 105A and a second detection line 105B;

[0065] The first detection line 105A and the second detection line 105B are arranged in parallel with an interval of 8 mm.

[0066] Fluorescent microspheres are coated on the sample pad 104, and the...

Embodiment 2

[0073] This embodiment provides the assembly method of the combined detection test strip for respiratory syncytial virus, respiratory adenovirus and parainfluenza virus, such as figure 2 Shown:

[0074] The nitrocellulose membrane 201 connected with absorbent paper is divided into two parts: absorbent paper 202 and nitrocellulose membrane 203, and the sample pad 204 is combined with one end of the nitrocellulose membrane 203 to obtain respiratory syncytial virus, respiratory adenovirus and Parainfluenza virus joint detection test strip 206.

[0075] The side of the sample pad 204 and the absorbent paper-attached nitrocellulose membrane 201 facing away from the detection line is also provided with a coating film and a backing.

[0076] Wherein the nitrocellulose membrane 203 is provided with a first detection line 205A, a second detection line 205B and a third detection line 205C;

[0077] The first detection line 205A, the second detection line 205B and the third detection ...

Embodiment 3

[0085] This embodiment provides a method for assembling and using a respiratory pathogen immunochromatography multiplex detection reagent card. The respiratory pathogen immunochromatography multiplex detection reagent card can be used for influenza A virus, influenza B virus, respiratory syncytial Joint detection of 5 items of virus, respiratory adenovirus and parainfluenza virus. For details, please refer to image 3 Shown:

[0086] The card case 301 has a place for placing a test paper, a sample loading area 302 and a connecting channel 303 ;

[0087] It should be noted that the shape of the sample loading area 302 can be set as an ellipse as shown in the figure, or can be set as a long strip or a rectangle.

[0088] Preferably, the connecting channel 303 can also be arranged inside the card case 301, and only two open rear ends are respectively connected to the sample adding area 302 and the card position for placing the test paper.

[0089] Put the combined detection te...

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Abstract

The invention relates to the field of medical examination, in particular to a multi-connected detection reagent card adopting immunochromatography and used for respiratory pathogens. The multi-connected detection reagent card comprises multiple sample pads and multiple nitrocellulose membranes connected with absorbent paper, wherein each sample pad is covered with multiple fluorescent microspheres, and the surface of each fluorescent microsphere is covered with an antibody of one respiratory pathogen; each nitrocellulose membrane connected with the absorbent paper is provided with at least two detection lines, and the detection lines are arranged at the nitrocellulose membrane; each detection line is fixedly covered with an antibody of one respiratory pathogen, and the antibody is in a pairing relation with the antibody, covering the surface of one fluorescent microsphere covering the corresponding sample pad, of one respiratory pathogen. The reagent card is simple, convenient and rapid to operate; different specific test strips can be formed through matching of different nitrocellulose membranes connected with the absorbent paper and the sample pads, different test strips are matched in a mixed manner and put in card paper according to demands, the detection flexibility and the customization property are high, and the detection cost is saved.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a respiratory pathogen immunochromatography multiple detection reagent card. Background technique [0002] Respiratory tract infection refers to pathogens infecting the respiratory system such as the nasal cavity, throat, trachea and bronchi of the human body. Divided into upper respiratory tract infection and lower respiratory tract infection, the common upper respiratory tract infection is acute upper respiratory tract infection (acute upper respiratory tract infection), which refers to the general term for acute inflammation of the nasal cavity, pharynx or larynx, and is the most common infectious disease of the respiratory tract. The common cause is a virus, and a few are caused by bacteria. Patients regardless of age, gender, occupation and region. Not only is it highly contagious, but it can also cause serious complications. Lower respiratory tract infection is the most co...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/569
Inventor 肖江群江应玲钟乾兴王保丹乐宜萃
Owner ANBIO XIAMEN BIOTECHNOLOGY CO LTD
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