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Tissue culture and rapid propagation breeding method for Corylus heterophylla Fish. and Corylus avellana L. hybrid

A Pingou hybrid hazelnut and rapid breeding seedling technology, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow growth of test-tube seedlings, difficulties in tissue culture, and low reproduction efficiency, and achieve a strong and neat root system. Effects of shortening the seedling raising cycle and simple and easy method

Inactive Publication Date: 2016-11-09
临沂真源观光农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the hazelnut tree contains more tannins, its tissue culture is very difficult, and it is prone to problems such as high pollution rate, easy vitrification, low reproductive efficiency, and slow growth of test-tube plantlets during the cultivation process, which limit the industrial production of test-tube plantlets. Therefore, there are few reports on the success of hazelnut tissue culture at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Explant Selection, Disinfection Treatment and Induction Culture of Pingou Hybrid Hazelnut's Tissue Culture Rapid Propagation Seedlings

[0020] Select the young shoots of Pingou hybrid hazel that are free from diseases, insect pests and mechanical damage in the field, trim them into stems with 1-2 axillary buds, rinse them with running water, and then soak them in 70% alcohol solution under sterile conditions 30s, then sterilized with 0.1% mercuric chloride for 8 minutes, and finally soaked in sterile water for 6 to 7 times, each time for 1 minute, and used as explants;

[0021] The explants were inoculated on the induction medium and placed in a constant temperature biochemical incubator for cultivation; the specific conditions for cultivation were: temperature 25±2°C, humidity 60%-70%, light time 12h / d, light intensity 1000LX and cultivated for 15 -25 days. The Pingou hybrid hazel explants selected from the same species were placed in different induction medium condi...

Embodiment 2

[0026] Proliferation culture

[0027] The clustered shoot tips induced under the optimized conditions in Example 1 were transferred to different proliferation media for proliferation culture. The proliferation culture conditions were: temperature 23±2°C, humidity 60%-70%, light time 10-13 h / d, light intensity 1500 ~ 2000LX and culture for 20-30 days. The specific cultivation conditions are shown in Table 2.

[0028] Table 2 Effects of different proliferation medium formulations on the proliferation and growth of sprouts

[0029] Variety

[0030] It can be seen from Table 2 that the formula of Pingou hybrid hazelnut tissue culture value-added medium is WPM+ 6-BA 4.0mg / L, 2,4-D0.7mg / L, AC 1.0g / L, 500 mg / L hydrolyzed casein , 300mg / L amino acid chelated iron, sucrose 30 g / L, agar 6.0g / L, when pH 5.8, its multiplication coefficient reaches 6.5, and multiplication coefficient is all lower in other culture medium formulas, and this is because the present invention The p...

Embodiment 3

[0032] rooting culture

[0033] The sprouts that grow to 5cm in the multiplication medium optimized in Example 2 are transferred to different rooting mediums for rooting culture. The rooting culture conditions are: temperature 25 ± 2 ° C, humidity 70% ~ 75%, light time 12-14 h / d, light intensity 2000-3000 LX and culture for 30 days. The specific results are shown in Table 3.

[0034] Table 3 Effects of different rooting medium formulations on the rooting of test-tube plantlets

[0035] Variety

[0036] As can be seen from the data in table 3, under the same situation of basic medium, the plant growth regulator added is too high or too low to be unfavorable for sprouts to take root, thus the optimal formulation of sprouts' rooting medium of the present invention consists of : 1 / 2MS+BA0.5mg / L+NAA0.12mg / L+IAA 0.3mg / L, pH 5.8. In addition, the present invention also uses the optimized culture medium formula in the rooting cultivation process of two varieties of "Dawei...

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Abstract

The invention discloses a tissue culture and rapid propagation breeding method for Corylus heterophylla Fish. and Corylus avellana L. hybrid, which belongs to the technical field of agriculture. The seedling growing method comprises 1) explant selection and disinfection; 2) induction culture; 3) multiplying tissue culturing rapid propagation cultivation; 4) rooting culture; and 5) domestication and transplanting. A system of the invention performs researches on the tissue culture and rapid propagation breeding method for Corylus heterophylla Fish. and Corylus avellana L. hybrid, conversion from laboratory scale to industrialization scale is realized; seedling growing cost is reduced, hazelnut breeding theory is enriched, high quality rapid breeding is realized; rooting rate of Corylus heterophylla Fish. and Corylus avellana L. hybrid can reach 98%, transplanting survival rate can reach about 95%, and the tissue culture and rapid propagation breeding method has good application prospect.

Description

technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to a method for tissue culture and rapid propagation of Pingou hybrid hazelnut seedlings. Background technique [0002] Hazelnut is an important woody oil tree species and one of the four major dry nuts in the world. The main cultivated species in the world comes from Hazelnut. Hazelnut is known as the "King of Nuts" because of its rich nutritional value and high economic value. At present, the hazelnuts promoted in China are the hybrid varieties of Pinghe and European hazel, called Pingou hybrid hazel. Pingou hybrid hazelnut is an excellent hazelnut variety with the characteristics of large fruit, high yield, cold resistance and drought tolerance. [0003] According to statistics, my country's hazelnut market demand has a gap of more than 1 million tons. Vigorously developing the hazelnut industry is a breakthrough point to solve the contradiction between supply and...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王宗岭
Owner 临沂真源观光农业科技有限公司
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