Compound microorganism preparation for preventing and treating rice blast and preparing method of compound microorganism preparation

A technology of composite microorganisms and rice blast, which is applied in the field of composite microbial preparations and preparations for the prevention and treatment of rice blast, can solve the problems of single mode of action, unsatisfactory production and use, unstable control effect, etc., and achieve pertinence Strong, super strong control of rice blast, high efficacy

Inactive Publication Date: 2016-11-09
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Guo Jianhua et al. disclosed a biocontrol strain JH21 and its biocontrol agent for preventing and treating rice sheath blight and rice blast in Patent No. 2011100743369; Bacillus strains; due to the diversity and simultaneous evolution of pathogenic bacteria, the existing single anta

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A compound microbial preparation for preventing and treating rice blast. The raw material ratio of the microbial preparation is: 30 parts of fermentation broth of Streptomyces tsushima, 40 parts of fermentation broth of Streptomyces syringae, 30 parts of fermentation broth of Bacillus arborii, and brevibacillus molybdenum 30 parts of Bacillus fermentation liquid.

[0031] In order to achieve the above object, the preparation method of the compound microbial preparation for preventing and treating rice blast comprises the following steps:

[0032] Step 1: Preparation of Streptomyces tsushima fermentation broth

[0033] Take out the preservation tube of Streptomyces tsushima strain, use Gaoshi No. 1 solid medium to scratch the plate for recovery, and culture at 30°C for 7 days. Pick a single colony under the plate and inoculate it into an eggplant bottle with 150 ml of Gaoshi No. 1 solid medium, and culture it in an incubator at 30°C for 6-8 days. When the lawn is overgr...

Embodiment 2

[0052] Embodiment 2 plate antibacterial test

[0053] According to the plate confrontation culture test method in the "Plant Disease Research Method", the compound microbial preparation prepared in Example 1 is evaluated on the inhibitory effect of the rice blast bacterium, and when the clear water contrast treatment group is covered with the plate, the statistical results are as follows, the 44%。 Composite microbial preparations growth inhibition rate of blast fungus was 95.44%.

Embodiment 3

[0054] Example 3 Composite microbial preparation is tested in rice blast greenhouse

[0055] 6.7m per plot 2 , three repetitions; rice plants were inoculated with pathogenic bacteria when they reached the tillering stage;

[0056] After inoculating the spores of Magnaporthe grisea, spray 30ml of compound microbial preparation for the first time when the control group begins to morbidity, dilute it 100 times and spray, and then spray the bacterial preparation once every 7-10 days, three times in a row. Until the harvest of rice, investigate the control effect and yield; 20% tricyclazole WP (Jiangsu Fengdeng Agrochemical Co., Ltd.) was used as the control agent. The blank control group was sprayed with water each time.

[0057] Test results: As shown in Table 1, the control effect of the compound microbial preparation on rice blast was 90.74% in the greenhouse test, much higher than that of the tricyclazole treatment group. At the same time, the use of this composite microbia...

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PUM

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Abstract

The invention discloses a compound microorganism preparation for preventing and treating rice blast and a preparing method of the compound microorganism preparation. The compound microorganism preparation is prepared from streptomyces tsusimaensis fermentation liquor, syringa oblata streptomycete fermentation liquor, asburiae bacillus fermentation liquor and beautiful Bacillus pumilus fermentation liquor. According to strains of the compound microorganism preparation, rice can be induced to generate disease resistance through the comprehensive utilization of interspecific or intraspecific antibiosis, competition, hyperparasitism and bacteriolysis of microorganisms and through secondary metabolite, various antibiotics such as streptomyces sparsogenes, nonactin and valinomycin can be generated, and a superstrong suppression effect can be generated on pathogenic bacteria of rice blast; the four strains used in the compound microorganism preparation can be directly separated and obtained from soil, all have the root trace growth-promoting effect, can breed and grow on the surfaces of crops or inside plants or in soil while secreting certain secondary metabolite towards the rhizosphere of plants in an orientation mode, and can improve absorption of plants to nutritive substances, stimulate the growth of plants and perform the role of fertilizer.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological control and application of microbial agents, and in particular relates to a compound microbial agent for preventing and treating rice blast and a preparation method thereof. Background technique [0002] Rice blast, also known as rice fever, knocking head blast, etc., is a worldwide fungal disease that has caused great harm to global rice production. Diseases occur in all rice areas in my country, and generally cause a 10% to 20% reduction in production in the year when the disease is prevalent, and a 40% to 50% reduction in production in the year of severe disease, or even no harvest. Since the 1990s, the annual occurrence area of ​​rice blast in my country has been more than 3.8 million hm2, and the annual loss of rice has reached hundreds of millions of kilograms. In terms of production, my country currently mainly uses chemical pesticides to prevent and control rice blast. Gene...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01C12R1/07C12R1/465
CPCA01N63/10C12N1/20
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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