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Solvent for dissolving agarose at low temperature and method for preparing agarose gel

An agarose and solvent technology, which is applied in the fields of natural polymers, environmental engineering, agriculture, and chemistry, can solve the problems of limited application, limited derivatization reaction and processing, instability, etc., and achieves obvious innovation and outstanding room temperature stability. Effect

Inactive Publication Date: 2018-08-21
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The agarose solution obtained by heating in this method becomes extremely unstable once cooled below 70 degrees Celsius, limiting its further derivatization and processing at room temperature
At the same time, the agarose gel obtained by cooling after dissolving in boiling water shows obvious brittleness and poor mechanical properties, which limits its application in a wider range of fields.

Method used

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  • Solvent for dissolving agarose at low temperature and method for preparing agarose gel
  • Solvent for dissolving agarose at low temperature and method for preparing agarose gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Weigh 3 grams of agarose powder, add 100 grams of 5 wt% lithium hydroxide monohydrate / 10wt% urea mixed aqueous solution, stir well and put it in the refrigerator to freeze, then stir while thawing, fully melt and centrifuge at 6000rpm for 5 minutes , to obtain a transparent agarose solution.

Embodiment 2

[0018] Weigh 3 grams of agarose powder, add 100 grams of 8 wt% lithium hydroxide monohydrate / 20 wt% urea mixed aqueous solution, stir thoroughly and place in the refrigerator to freeze, then stir while thawing, fully melt and centrifuge at 6000rpm for 5 minutes to obtain a transparent agarose solution.

Embodiment 3

[0020] Weigh 6 grams of agarose powder, add 100 grams of 7 wt% lithium hydroxide monohydrate / 13 wt% urea mixed aqueous solution, vortex and disperse, freeze in the refrigerator, then thaw at room temperature while stirring, and centrifuge at 10,000 rpm After degassing for 5 min, a clear and transparent agarose solution was obtained. Pour the centrifuged solution into the prepared mold, put it into 90% ethanol aqueous solution to solidify and form, and then wash repeatedly to remove excess alkali and urea to obtain a regenerated agarose gel. It can be seen from the scanning electron microscope image of the gel that the surface of the gel is smooth and flat, and has very good uniformity.

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Abstract

The invention discloses an agarose solvent and an application of the agarose solvent to agarose gel preparation. Lithium hydroxide and urea are dissolved in water according to a certain proportion, so that the solvent can be prepared. The solvent is mixed with a certain weight of agarose, and agarose solution with needed concentration can be obtained by a low-temperature freezing-thawing method. The agarose solution dissolved by the solvent has excellent stability at room temperature. The solution is centrifugally defoamed and then regenerated by coagulation in a coagulating bath, so that regenerated agarose gel can be prepared. The solution can be directly used for chemical cross-linking to obtain chemical gel. Compared with traditional agarose gel obtained by hot water dissolution, the gel has better mechanical property. The solvent and the gel preparation method are wide in condition, green and environmentally friendly and have an important application prospect, and a completely new path is opened up for dissolution and use of the agarose.

Description

technical field [0001] The invention relates to a solvent for dissolving agarose at low temperature and a method for preparing agarose gel, which belong to the field of natural polymers, and also belong to the fields of chemistry, agriculture and environmental engineering. Background technique [0002] At present, in order to solve the problems of depletion of petroleum resources and white pollution caused by non-degradable plastics, the development of science and technology pays more and more attention to the development and utilization of renewable biomass resources. Agarose is the product of removing agar pectin from agar, which is derived from marine organisms and is a relatively abundant biomass resource in marine organisms. It comes from natural sources, can be degraded by microorganisms, can be regenerated and has excellent biocompatibility. It is widely used in biological analysis, food industry, environmental testing, chemical manufacturing, textile, national defen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/05C08J3/03C08J3/075C08J3/24C08L5/12
CPCC08J3/03C08J3/05C08J3/075C08J3/24C08J2305/12
Inventor 张俐娜汪森吕昂张瑶瑶
Owner WUHAN UNIV
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