Agaricus brasiliensis culture medium based on germinated mung bean embryos and preparation method of agaricus brasiliensis liquid spawn

Abstract

The invention discloses an agaricus brasiliensis culture medium based on germinated mung bean embryos. The medium includes the following components by weight: 140-145 parts of a malt extract, 180-182 parts of germinated mung bean embryos, 8-9 parts of selenium-enriched yeast, 25-30 parts of small molecular squid peptide, 29-30 parts of pentose, 4-5 parts of catechin, 3-4 parts of monosodium glutamate, 5.6-6 parts of nanometer tourmaline, and 6.2-6.5 of bacterial nano cellulose. The invention adopts the malt extract, germinated mung bean embryos, small molecular squid peptide and pentose as culture medium for the preparation of agaricus brasiliensis liquid spawn; the medium is rich in nutrients, and through extraction and stacking fermentation, the nutrients in the medium become small molecules, which can be effectively absorbed and utilized by the agaricus brasiliensis liquid spawn pellets; and the nano bacterial cellulose improves the stability and uniformity of the culture medium, and provides a good space and ingredients for the growth of agaricus brasiliensis liquid spawn.

Description

technical field [0001] The invention relates to the technical field of liquid strains, in particular to a Agaricus blazei culture medium based on germinated mung bean embryos and a method for preparing Agaricus blazei liquid strains. Background technique [0002] In the production process of Agaricus blazei, liquid strains have incomparable advantages over solid strains. Liquid strains have short production cycle, short and consistent bacterial age, high purity, strong vitality, simple and quick inoculation, and easy industrialization and large-scale production. , standardized production and other advantages. Now, more and more people are researching and using liquid strains, and the promotion and use of liquid strains is also an inevitable trend to realize industrialization, scale and standardization of edible fungi. However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually grows quickly, but the penetrating po...

AI Technical Summary

Problems solved by technology

However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually grows quickly, but the penetrating power is not strong. If the compost is too thick, mycelium is not easy to penetrate the compost, and the mycelia will follow the compost. The surface grows rapidly, and the amount of mycelium inside the compost is very small, which seriously affects the yield and quality of the strain; 2. Although the conventional liquid strain solves some problems existing in the solid strain, it can significantly shorten the cultivation cycle and enhance the growth rate of the strain. Species vigor and stress resistance, reducing the contamination rate of strains, and improving the uniformity of strain quality, but there are still some deficiencies such as the density, size, and uniformity of the bacterial balls in the liquid strains obtained by fermentation are not very ideal. If the bacteria balls are crushed by stirring and shearing, the uniformity of the obtained strains will be more ideal. However, the shear force of high-speed stirring, crushing and beating will greatly damage the strains and have a serious impact on the overall quality of the strains.

Publication No. CN105309194A "New Technology for the Production of Edible Mushroom Microparticle Liquid Strain" provides a kind of edible fungus microparticle liquid strain with a mycelium ball particle diameter of 0.5mm, which increases the ventilation rate of the edible fungus liquid strain culture solution, so that the ventilation rate It is suitable at 1:0.6—0.9V / V·min. At the same time, a stirrer is installed in the aerated fermenter to make the eddy current of the culture liquid move and prolong the technical operation method of the air bubble. The method for producing the microparticle liquid strain is from the production process There is no improvement in the aspect of liquid culture medium, and the implementation is technically difficult and demanding; Publication No. CN105130516A "Method for Making Molecular-level Biological Medium" mentions that the nutrients of molecular-level biological medium can be absorbed quickly and comprehensively. Promotes the growth of liquid strains, but does not mention the effect of molecular-grade biological media on the diameter of liquid strain mycelium balls