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Method for degrading large-molecular weight polysaccharide containing beta-1,3-glucosidic bonds

A technology with large molecular weight and glycosidic bonds, which is applied in the field of degrading large molecular weight polysaccharides containing β-1,3-glycosidic bonds, can solve problems such as unsuitable for industrialized expansion of production, destructive product structure and activity, and low degradation rate. Strong cancer cell activity, scale-up production, and the effect of inhibiting cancer cell activity

Active Publication Date: 2016-11-23
北京颐方生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cleavage of glycosidic bonds in the acid degradation method is random, and the molecular weight distribution of the product is wide, which is destructive to the structure and activity of the product.
The hydrogen peroxide degradation method requires the use of hydrogen peroxide, which is potentially dangerous during storage and transportation, and has a low degradation rate, so it is not suitable for industrialized expansion of production

Method used

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  • Method for degrading large-molecular weight polysaccharide containing beta-1,3-glucosidic bonds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]A method for degrading lentinan by using the electric Fenton method: take 1600g lentinan (molecular weight 500KDa), disperse it in 1.6Kg absolute ethanol, add it to 10L distilled water, make a concentration of sugar solution that is 160mg / ml, pack In an electrolytic cell with a volume of 30 L, weigh 85.2 g of sodium sulfate and dissolve it in the polysaccharide solution, and adjust the pH to 3 with 1 mol / L hydrochloric acid. Use 30cm×20cm barbed wire as the anode, 30cm×20cm porous graphite as the cathode, the distance between the two electrodes is 6cm, and 13V direct current is applied, and the current density is 10mA / cm 2 , and use the aeration device to feed in purified air at a speed of 2.0L / min (or to feed in oxygen at a speed of 0.6L / min, the aeration device contains two vent holes, and the two vent holes are blown into oxygen at the same time), per liter The amount of air introduced into the thick lentinan solution is about 24L (the amount of air introduced into th...

Embodiment 2

[0036] A method for degrading lentinan by using the electric Fenton method: take 900g lentinan (molecular weight 500KDa), disperse it in 0.72Kg dehydrated alcohol, add it to 10L distilled water, and make a sugar solution with a concentration of 90mg / ml. In an electrolytic cell with a volume of 30 L, weigh 71 g of sodium sulfate and dissolve it in the polysaccharide solution, and adjust the pH to 3 with 1 mol / L hydrochloric acid. With 20cm×20cm barbed wire as the anode and 20cm×20cm porous graphite as the cathode, the distance between the two electrodes is 5cm, and 13V direct current is applied, and the current density is 12mA / cm 2 , and use the aeration device to feed oxygen at a rate of 0.5L / min. The aeration device contains two vent holes, and the two vent holes are blown into oxygen at the same time. The amount of air per liter of crude lentinan solution is about 4.0L. The stirrer was stirred at a speed of 250rpm. After electrolysis at room temperature for 40 minutes, the p...

Embodiment 3

[0038] A kind of method that adopts electric Fenton method to degrade polysaccharides: take 2000g polysaccharides (average molecular weight 452KDa), be dispersed in 2.4Kg dehydrated alcohol, join in 10L distilled water, make the sugar solution that concentration is 200mg / ml, pack into an electrolytic cell with a volume of 30 L, weigh 99.4 g of sodium sulfate and dissolve it in the polysaccharide solution, and adjust the pH to 3 with 1 mol / L hydrochloric acid. Use 30cm×30cm barbed wire as the anode, 30cm×30cm porous graphite as the cathode, the distance between the two electrodes is 5cm, 13V direct current is connected, and the current density is 14mA / cm 2 , and use an aeration device to feed oxygen at a rate of 0.6L / min. The aeration device contains two vent holes, and the two vent holes are blown into oxygen at the same time. The amount of air per liter of crude lentinan solution is about 7.2L. The stirrer was stirred at a speed of 250rpm. After electrolysis at room temperatu...

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Abstract

The invention relates to a method for degrading large-molecular weight polysaccharide containing beta-1,3-glucosidic bonds. The method comprises the specific steps that a large-molecular weight polysaccharide solution containing beta-1,3-glucosidic bonds with the concentration being 60-200 mg / mL is introduced into an electrolytic cell provided with cathodes and anodes, the solution is adjusted to be acid, oxygen is introduced into the cathode on the basis of the amount of the 4.0-8.0 L oxygen / L polysaccharide solution, direct current is introduced and controls the current density of the cathode to be 9-14 mA / cm<2>, on the condition, degradation is performed, and an electrolyte is collected. The method is simple technology and easy to implement, time is saved, the method is efficient, and large-scale production is promoted; reaction conditions are mild, and environment pollution is small; importantly, compared with acid process degradation and hydrogen peroxide degradation method, the polysaccharide degradation rate is high through the electric Fenton method, and the range of the obtained low-molecular-weight polysaccharide molecular weight is narrower on the condition that the polysaccharide of equivalent molecular weight is degraded.

Description

technical field [0001] The invention relates to the field of production of pharmaceutical raw materials, in particular to a method for degrading large-molecular-weight polysaccharides containing β-1,3-glucosidic bonds. Background technique [0002] Polysaccharide is formed by the condensation and dehydration of multiple monosaccharide molecules. It is a kind of carbohydrate with complex molecular structure and huge size. Active polysaccharide refers to a polysaccharide compound with special physiological activity, which has the function of regulating the circadian rhythm of the human body. Monosaccharides in polysaccharides are connected by glycosidic bonds, and glycosidic bonds include β-1,4-glycosidic bonds, α-1,3-glycosidic bonds, β-1,3-glycosidic bonds, etc. [0003] Studies have shown that the biological activity of polysaccharides is closely related to its molecular weight. The larger the molecular weight, the larger the molecular volume, and the poor solubility. lear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C25B3/00A61K31/716A61P31/12A61P35/00A61P37/02
CPCA61K31/716A61P31/12A61P35/00A61P37/02C08B37/0024C25B3/00
Inventor 石清东王姣
Owner 北京颐方生物科技有限公司
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