A kind of Streptomyces flexneri solid culture medium, culture method and preparation method of agricultural microbial bacterial agent

Abstract

The invention provides a streptomyces fradiae solid culture medium, a culture method and a preparation method of an agricultural microbial agent, belonging to the field of microbial fermentation. The streptomyces fradiae solid culture medium is prepared from the following components in parts by weight: 3-10 parts of corn flour, 3-18 parts of soybean meal, 18-45 parts of straw powder, 10-30 parts of turfy soil, 20-50 parts of phosphate tailing, 1-10 parts of calcium carbonate and 40-50 parts of nutrient solution. According to the invention, a solid fermentation culture medium is prepared through liquid-solid two-phase fermentation by adopting the solid culture medium and is used for producing an agricultural streptomyces fradiae agent. The fermentation culture medium for streptomyces fradiae spore production, provided by the invention, is suitable for the growth of streptomyces fradiae, and over 7.5*10<9> spores can be produced from each gram of fermentation raw material; and the germination rate of the prepared spore powder can reach 95%, the prepared agricultural microbial agent conforms to the national standard of agricultural microbial agent, and an obvious promotion effect is realized on the crop growth.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a Streptomyces flexneri solid culture medium, a preparation and fermentation method thereof, and a method for preparing an agricultural Streptomyces flexneri bacterial agent using the culture medium. Background technique [0002] Streptomycetaceae (Streptomycetaceae) is the main producer of antibiotics. Among the more than 20,000 active substances produced by microorganisms, Streptomycetaceae accounts for more than 60%. Application prospect. As a species of Streptomyces, Streptomyces freundii can produce effective active ingredients such as tylosin and neomycin, which have obvious growth-promoting and disease-resistant effects on crops, and are increasingly used in Fruit trees, vegetables and other crops are being cultivated. [0003] However, the amount of Streptomyces flexneri growing naturally in the soil is too small to perform the above-mentioned effects. In the prio...

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Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a Streptomyces flexneri solid culture medium and its preparation and fermentation method, as well as a method for preparing the agricultural Streptomyces flexneri inoculant using the medium, so as to solve the problem of liquid fermentation in the prior art. Streptomyces freundii has less spore production, long fermentation cycle, difficulty in preparing agricultural solid microbial inoculum, and technical problems in effective utilization of phosphate rock resources

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