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An optimized antioxidant decellularized protection solution

A technology of decellularization and protection solution, which is applied in the field of optimized anti-oxidation decellularization protection solution and its preparation, which can solve the problems of low self-degradation performance and poor tissue compatibility

Active Publication Date: 2018-01-12
拜欧迪赛尔成都生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, artificially synthesized polymer materials are mostly used in clinical tissue transplantation and repair, which often have the problems of low self-degradation performance and poor tissue compatibility. urgent

Method used

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  • An optimized antioxidant decellularized protection solution
  • An optimized antioxidant decellularized protection solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of Antioxidant Decellularized Protective Solution:

[0019] 1. Take 10g of DMEM powder and add 500ml of deionized water, dissolve and sterilize by autoclaving;

[0020] 2. Take 25g of chondroitin sulfate, add 275ml of deionized water, heat, dissolve and autoclave to make a chondroitin sulfate solution; add 20g of low-molecular-weight dextran to 100ml of water for injection to dissolve and autoclave to make a low-molecular-weight dextran solution; L - Histidine hydrochloride 2.87g, add 100ml of water for injection to dissolve and autoclave to make L-histidine hydrochloride solution;

[0021] 3. Take a sterile container and add 500ml of DMEM culture solution, 275ml of autoclaved chondroitin sulfate, 100ml of low molecular weight dextran solution, 100ml of L-histidine hydrochloride solution; add 0.5g of allopurinol, hydroxypropyl methylcellulose 5g glutathione, 2g reduced glutathione, 2mg dexamethasone injection, 0.1g levofloxacin injection and mix well;

[0...

Embodiment 2

[0026] Observation and detection of decellularization effect and tissue structure of porcine tendon tissue using acellular protection solution

[0027] Take out the tendon tissue within 3 hours after the fresh pig is slaughtered, aseptically treat it, incise the aponeurosis to make 1 cm × 2 cm tissue slices, take 5 slices and seal them in a plastic bag containing 10 ml of the preservation solution prepared in Example 1; In the other control group, 5 tissue pieces were sealed in a plastic bag filled with 10ml BSS. Under the condition of 600MP high static pressure, treat 8 times, each time is 3 minutes; after taking out the ligament, place it in the protective solution containing 0.2% sodium lauryl sulfate + 1000U / ml DNase, the temperature is 25 °C, set the shaker speed to 100 rpm, and treat for 2 hours; take out the decellularized tendon and rinse it in the protective solution for 2 hours. Enzyme and detergent and final rinse treatments of the control group were carried out in...

Embodiment 3

[0030] Observation and detection of decellularization effect and tissue structure of conjunctival tissue using acellular protection solution

[0031] Take out the conjunctival tissue within 2 hours after the fresh pig is slaughtered, remove the tissue under the conjunctiva as much as possible, and perform aseptic treatment, cut the conjunctival tissue into 0.5cm×1cm tissue pieces, of which 5 pieces of conjunctival tissue are sealed in a 10ml container for implementation In the plastic bag of the preservation solution prepared in Example 1; in the other control group, 5 pieces of conjunctival tissue were sealed in a plastic bag filled with 10ml BSS. Under the condition of 300MP high static pressure, treat 3 times, each time is 1 minute; after taking out the conjunctival tissue, put it in the protective solution containing 0.2% sodium dodecyl sulfate + 1000U / ml DNase, the temperature at 25°C, set the shaker speed to 100 rpm, and treat for 1 hour; remove the decellularized conjun...

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Abstract

The invention relates to an optimized anti-oxidative accelular protective solution, which has a critical effect on protection of structural completeness and biological nature of acellular tissues in the whole course of the decellularizing process. The protective solution is prepared from DMEM cellular culture medium L-histidine hydrochloride, allopurinol, chondroitin sulfate, low molecular dextran, hydroxypropyl methyl cellulose, HEPES buffer solution, dexamethason hydrochloride, reduced glutathione and levofloxacin. The protective solution is a light-red liquid with specific pH value, specific crystal and colloid osmotic pressure. The optimized anti-oxidative accelular protective solution is easily available in raw materials and economical in price, has strong applicability, wide application range and excellent anti-oxidation performance, and has a protective effect in the process of decellularizing biological tissues.

Description

technical field [0001] The invention belongs to the technical field of chemical compositions, and in particular relates to an optimized anti-oxidation and decellularization protective solution, its preparation method and application. Background technique [0002] The 21st century is a century of new developments in biological sciences. As a rising sun in the field of biomedicine in the past 10 years, TERM (Tissue Engineering & Regenerative Medicine) has attracted worldwide attention. In the research and application of TERM, related decellularization technology occupies a pivotal position. Decellularization refers to the process of separating extracellular mesenchyme from cells in the field of biomedical engineering. The separated extracellular mesenchymal scaffold can be used for artificial organs and tissue regeneration. Using physical, chemical, enzymatic treatment and a combination of the three methods is currently the most widely used decellularization method in the wor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 史真史伟云
Owner 拜欧迪赛尔成都生物科技有限公司
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