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Production method of forage-use mannose oligomer

A technology of a mannose oligosaccharide and a production method is applied in the field of production of mannose oligosaccharides for feed, and can solve the problems of difficulty in releasing mannose, waste, restricting the utilization of raw materials, etc. The effect of improving the comprehensive utilization value

Inactive Publication Date: 2016-12-07
SHANGHAI LAMP BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the hard structure of these mannose-derived raw materials, it is difficult to release mannose by common methods, which also limits the further utilization of these raw materials, and currently they can only be wasted or simply used as one of the feed raw materials, and the utilization rate is very low

Method used

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  • Production method of forage-use mannose oligomer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] For the processing of palm kernel meal, see the flow chart of the steps figure 1 .

[0024] 1) Take 1kg of palm kernel meal and pass through sieving, winnowing, and permanent magnetization, remove the impurities therein, and pulverize it to about 125 mesh through an ultrafine pulverizer.

[0025] 2) After adding 5 times of water to the ultra-finely pulverized palm kernel meal, stir evenly, enter the high-pressure homogenizer, and process it at 1000bar,

[0026] 3) Add 1 times water to the material after high-pressure homogenization, stir evenly, enter the ultra-high pressure homogenizer, and process it at 1800bar,

[0027] 4) Heating the two homogenized materials to 63°C, adjusting the pH to 3.8 with 10% calcium hydroxide solution or 10% sulfuric acid solution, and enzymatically hydrolyzing at constant temperature for 20 hours under stirring.

[0028] 5) After enzymatic hydrolysis, solid-liquid separation was performed, and the solid was directly dried to obtain a dry...

Embodiment 2

[0032] Processing of coconut meal

[0033] 1) Take 500g of coconut meal and pass through sieving, winnowing, and passing through permanent magnetism, remove impurities therein, and pulverize it to about 150 mesh through an ultrafine pulverizer.

[0034] 2) After adding 4 times of water to the ultra-finely pulverized coconut meal, stir evenly, enter the high-pressure homogenizer, and process at 1000bar.

[0035] 3) Add 1 times water to the material after high-pressure homogenization, stir evenly, enter the ultra-high pressure homogenizer, and process at 1800bar.

[0036] 4) Heating the two homogeneously treated materials to 60°C, adjusting the pH to 3.5 with 10% calcium hydroxide solution or 10% sulfuric acid solution, and enzymatically hydrolyzing at constant temperature for 20 hours under stirring.

[0037] 5) After the enzymatic hydrolysis, solid-liquid separation was performed, and the solid was directly dried to obtain a dry residue weighing 0.34 kg.

[0038] 6) After th...

Embodiment 3

[0041] Processing of coffee grounds

[0042] 1) Take 1kg of coffee grounds and pass through sieving, winnowing, and permanent magnetization to remove impurities and crush them to about 100 meshes with a superfine pulverizer.

[0043] 2) After adding 5 times of water to the ultra-finely pulverized coffee grounds, stir evenly, enter the high-pressure homogenizer, and process at 1000bar.

[0044] 3) Add 1 times water to the material after high-pressure homogenization, stir evenly, enter the ultra-high pressure homogenizer, and process at 1800bar.

[0045] 4) Heating the two homogeneously treated materials to 65° C., adjusting the pH to 4 with 10% calcium hydroxide solution or 10% sulfuric acid solution, and enzymatically hydrolyzing at constant temperature for 20 hours under stirring.

[0046] 5) After the enzymatic hydrolysis, solid-liquid separation was performed, and the solid was dried directly to obtain a dry residue weighing 0.75 kg.

[0047] 6) After the solid-liquid sep...

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Abstract

The invention relates to a production method of a forage-use mannose oligomer. Raw materials adopt materials rich in the mannose oligomer. The production method comprises the following steps: a, removing impurities; b, carrying out ultramicro pulverization, wherein fineness requirement of the pulverized material is that 95% of the pulverized material can pass 0.15mm; c, carrying out high pressure homogenizing at a pressure lower than 20000psi; d, carrying out ultrahigh pressure homogenizing at the pressure of 20000-60000psi, so that the material is uniform and floccular; e, carrying out enzymatic hydrolysis by utilizing beta-mannase and other non-starch polysaccharide enzymes under the conditions that the temperature is 40-70 DEG C, pH is 3-5, addition amount of the beta-mannase accounts for 0.05-1% of the amount of the material and enzymatic hydrolysis time is 4-48 hours; f, carrying out filter pressing for separating solid and liquid; g, directly drying the solid to obtain dry residue; h, carrying out spray drying on the liquid, so that dry powder is obtained; and i, uniformly mixing the dry residue with the dry powder, and then smashing, so that the finished product is obtained. The production method of the forage-use mannose oligomer has the advantage that the raw material from which mannose is difficult to extract can become a material from which the mannose is easy to extract.

Description

[technical field] [0001] The invention relates to a production method of mannose oligosaccharides for feed. [Background technique] [0002] The existing mannose oligosaccharides are mainly derived from the cell walls of konjac and brewer's yeast. Konjac is not suitable for the feed industry due to its expensive raw materials and high product prices; the mannose content contained in the yeast cell wall is relatively low, and its production and application are relatively limited. [0003] Palm kernel meal, coconut meal, coffee grounds and other agricultural product processing by-products not only have high mannose content, but also have a wide range of sources and are cheap, so they are a good source. [0004] Due to the hard structure of these mannose-derived raw materials, it is difficult to release mannose by common methods, which also limits the further utilization of these raw materials. At present, they can only be wasted or simply used as one of the feed materials, and...

Claims

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Application Information

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IPC IPC(8): A23K40/10A23K20/163A23K10/14A23K10/37
CPCY02P60/87
Inventor 刘国庆赵肖钱晓勇
Owner SHANGHAI LAMP BIOLOGICAL SCI & TECH
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