Frozen gel three-dimensional structural body, preparation method and application thereof

A structure and ice glue technology, applied in the field of tissue engineering and three-dimensional printing, can solve the problems of difficult to build three-dimensional structure, uncontrollable internal structure, uncontrollable material distribution, etc. High volume and good mechanical properties

Active Publication Date: 2016-12-07
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing ice gel preparation technology relies on mold forming, and there are problems such as uncontrollable internal structure, uncontrollable material distribution, and difficulty in building large-scale three-dimensional structures and ensuring the survival of internal cells, etc.

Method used

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  • Frozen gel three-dimensional structural body, preparation method and application thereof
  • Frozen gel three-dimensional structural body, preparation method and application thereof
  • Frozen gel three-dimensional structural body, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Preparation of cryogel 3D structure by 3D printing with a single nozzle

[0069] 1. Sodium alginate powder (Sigma-Aldrich) was dissolved in deionized water to make a 3% homogeneous solution, and sterilized by filtering through 45 μm filter paper.

[0070] 2. Mix 2mL of sodium alginate solution prepared as above with 0.2mL of calcium chloride solution (100mmol -1 ) were uniformly mixed to form a precursor solution with a certain viscosity and loaded into a 1 mL disposable sterile syringe.

[0071] 3. Load the sterile syringe into the biological three-dimensional printing device (see Rui Yao, et al., In Vitro Angiogenesis of 3D Tissue Engineered Adipose Tissue, Journal of Bioactive and Compatible Polymer, 2009; 24:5), at room temperature, Under the control of computer software (Microsoft, AT640, Redmond, WA), with the stepper motor speed of 2mm / s and the scanning speed of 10mm / s, three-dimensional printing was performed on a sterile flat platform to form a volu...

Embodiment 2

[0076] Example 2 Preparation of cryogel three-dimensional structure by dual-nozzle three-dimensional printing

[0077] 1. Sodium alginate powder (Sigma-Aldrich) was dissolved in deionized water to make a 3% homogeneous solution, and sterilized by filtering through 45 μm filter paper.

[0078] 2. The gelatin powder is dissolved in deionized water to make a 10% homogeneous solution, which is stored at room temperature after repeated high-temperature sterilization.

[0079] 3. Mix 2 mL of the sodium alginate solution prepared above with 0.2 mL of the calcium chloride solution (100 mmol -1 ) were uniformly mixed to form a precursor solution with a certain viscosity and loaded into a 10 mL disposable sterile syringe.

[0080] 4. Load 2 mL of the gelatin solution prepared as above into a 10 mL disposable sterile syringe and store at 10° C. for 10 min to form a gelatin precursor solution with a certain viscosity.

[0081] 5. Load the sterile syringes loaded with the sodium alginate...

Embodiment 3

[0086] Example 3 Preparation of cell-loaded cryogel three-dimensional structure using collagen-containing cell culture medium

[0087] 1. Sodium alginate powder (Sigma-Aldrich) was dissolved in deionized water to make a 3% homogeneous solution, and sterilized by filtering through 45 μm filter paper.

[0088] 2. Mix 2mL of sodium alginate solution prepared as above with 0.2mL of calcium chloride solution (100mmol -1 ) were uniformly mixed to form a precursor solution with a certain viscosity and loaded into a 1 mL disposable sterile syringe.

[0089] 3. Load the sterile syringe into the biological three-dimensional printing equipment, under the control of computer software (Microsoft, AT640, Redmond, WA) at room temperature, with the parameter conditions of stepping motor speed 2mm / s and scanning speed 10mm / s 3D printing on a sterile flat platform to form a pregel 3D structure with a volume of 1cm / 1cm / 0.5cm (for the structure of the 3D printer, please refer to Rui Yao, et al.,...

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Abstract

The invention discloses a frozen gel three-dimensional structural body, a preparation method and an application thereof. According to the invention, a frozen gel tissue engineering scaffold of the complex three-dimensional structure is formed, and can be used for the fields of three-dimensional cell culture, in-vitro tissue construction, tissue engineering and regenerative medicine, pathology, pharmacological study and drug testing. and the three-dimensional printing frozen gel scaffold has the characteristics of personalization, customization, high cell loading, high porosity and permeation rate, adjustable aperture size, high elastic modulus and injectable transplantation. The raw materials of the frozen gel three-dimensional structural body comprise one or a plurality of mixture of sodium alginate, gelatin, collagen, matrigel, laminin and other biomaterials and its derivative.

Description

technical field [0001] The invention relates to tissue engineering and three-dimensional printing technology, in particular to a three-dimensional cryogel structure, its preparation method and application. Background technique [0002] The healthcare industry faces many pressing issues. For example, every year, a large number of patients with serious heart, kidney, liver, lung and other diseases are waiting for organ transplantation, and due to the limited sources of organs available for transplantation, most of these patients eventually die because they cannot wait for transplantation. Therefore, there is an urgent need for implantable tissues and organs suitable for wound repair, tissue augmentation, organ repair and organ replacement. In addition, there is a need for materials, tools and techniques that can facilitate the application of regenerative medicine and tissue engineering techniques. [0003] Three-dimensional printing (3D printing) technology is an emerging mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/22A61L27/20A61L27/50C12N5/00C12Q1/02
Inventor 姚睿孙伟黄御宇毛双双
Owner TSINGHUA UNIV
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