Method for utilizing feed raw material fibrous-bed bioreactor to ferment and produce butyric acid
A technology for bioreactors and feed materials, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of difficult product separation, long fermentation cycle, and high production costs, and achieve easy industrial scale-up and increase production. rate and yield, the effect of shortening the lag period
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Embodiment 1
[0026] What this embodiment provides is to use glucose as the carbon source to carry out the process of immobilizing Clostridium tyrobutyricum in a built-in rice bran fiber bed for batch fermentation of butyric acid. Stirred fermenter. Proceed as follows:
[0027] (1) Preparation of built-in rice bran fiber bed bioreactor: wash and dry the immobilized rice bran material, weigh 120g and place it in a 5L fermenter to form a built-in rice bran fiber bed bioreactor;
[0028] (2) Dip the Clostridium tyrobutyricum bacteria liquid with an inoculation needle, streak it on the enhanced medium plate containing 50mg / mL sodium butyrate, and culture it at 37°C for 72 hours;
[0029] The enhanced medium components include: glucose 30g / L, yeast extract 5g / L, peptone 5g / L, (NH 4 ) 2 S0 4 3g / L, K 2 HPO 4 1.5g / L, MgSO 4 ·7H 2 0 0.6g / L, FeS0 4 ·7H 2 0 0.03g / L, sodium butyrate 50mg / mL, culture conditions: 37°C, pH 6.0, anaerobic environment.
[0030] (3) Inoculate the single bacteriu...
Embodiment 2
[0036] What this embodiment provides is to use glucose as the carbon source to carry out the process of immobilizing Clostridium tyrobutyricum in a built-in wheat bran fiber bed to ferment butyric acid in batches, and the steps are as follows:
[0037] (1) Preparation of the built-in wheat bran fiber bed bioreactor: wash and dry the immobilized wheat bran material, weigh 120 g and place it in a 5L fermenter to form a built-in wheat bran fiber bed bioreactor;
[0038] (2) with embodiment 1 step (2);
[0039] (3) with embodiment 1 step (3);
[0040] (4) with embodiment 1 step (4);
[0041] When the sugar concentration in the fermentation broth is close to 0, the fermentation is stopped, and the final concentration of butyric acid in the fermentation broth reaches 19.87g / L. The kinetics of batch fermentation of butyric acid is as follows: image 3 shown.
Embodiment 3
[0043] like figure 1 As shown, the external feed material fiber bed bioreactor is to fill the immobilization material into the glass immobilization column (the column length is 31cm, the internal effective diameter is 5cm, with a jacket.), and the two ends are fixed by stainless steel wire mesh. The immobilized material does not leak out, and the bottom of the glass column is filled with a glass spring about 15-30mm high, the purpose is to make the fluid distribution even, and the two ends are sealed to form a fiber bed immobilization device 3, and it is compatible with the ordinary 5L mechanical stirring fermenter 1 Connected to form a closed circulation system, using a circulating constant temperature water bath to control the temperature of the immobilized column, forming an external feed raw material fiber bed bioreactor.
[0044] What this embodiment provides is to use glucose as the carbon source to carry out the process of immobilizing Clostridium tyrobutyricum on an ex...
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