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Microalgae biodiesel production method

A production method and technology of microalgae biology, applied in the field of microalgae biodiesel production, can solve the problems of low biodiesel yield, low catalytic efficiency, long reaction time, etc., achieve strong environmental adaptability, improve catalytic efficiency, and improve the reaction interface big effect

Inactive Publication Date: 2016-12-14
南宁华侨投资区政孙贸易有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing biodiesel production process generally has the following problems: low catalytic efficiency, low biodiesel yield, long reaction time, corrosion equipment, complex process, high energy consumption, high cost, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of Nannochloropsis medium: glucose 40mg / L, NaNO 3 1.0mg / L, KH 2 PO 4 1.4mg / L, chitosan 1.0mg / L, biogas slurry 7mg / L, f2 vitamin 1mL and seawater 1L;

[0027] A method for producing microalgae biodiesel, comprising the following steps:

[0028] S1. Nannochloropsis cultivation: take Nannochloropsis as raw material, and then inoculate it into the medium at an inoculation density of 5 million cells / mL, and irradiate it with a red and blue LED supplementary light for 8h / day. The ratio of red light to blue light is 1:2, the light intensity is controlled at 4000LX, the culture temperature is controlled at 24°C, and cultured for 8 days, the density of Nannochloropsis reaches a concentration of 70 million cells / mL, and the Nannochloropsis cells are collected. ;

[0029] S2. Oil extraction: after the Nannochloropsis cells collected in step S1 are concentrated and dried, ultrasonic waves are used to break the walls of the Nannochloropsis algae, the ultrasonic powe...

Embodiment 2

[0034] Preparation of Nannochloropsis medium: glucose 35mg / L, NaNO 3 1.1mg / L, KH 2 PO 4 1.5mg / L, chitosan 1.2mg / L, biogas slurry 8mg / L, f2 vitamin 2mL and seawater 1L;

[0035] A method for producing microalgae biodiesel, comprising the following steps:

[0036]S1. Nannochloropsis cultivation: take Nannochloropsis as raw material, and then inoculate the medium at an inoculation density of 6 million cells / mL, and irradiate it with a red and blue LED supplementary light for 10h / day, and the LED supplementary light The ratio of medium red light to blue light is 1:4, the light intensity is controlled at 3500LX, the culture temperature is controlled at 25°C, and cultured for 7 days, the density of Nannochloropsis reaches 70 million cells / mL, and the Nannochloropsis is collected. cell;

[0037] S2. Oil extraction: after the Nannochloropsis cells collected in step S1 are concentrated and dried, ultrasonic waves are used to break the walls of Nannochloropsis algae, the ultrasonic...

Embodiment 3

[0042] Preparation of Nannochloropsis medium: glucose 37mg / L, NaNO 3 1.2mg / L, KH 2 PO 4 1.6mg / L, chitosan 1.5mg / L, biogas slurry 9mg / L, f2 vitamin 1mL and seawater 1L;

[0043] A method for producing microalgae biodiesel, comprising the following steps:

[0044] S1. Nannochloropsis cultivation: take Nannochloropsis as raw material, and then inoculate the medium at an inoculation density of 5 million cells / mL, and irradiate it with a red and blue LED supplementary light for 14h / day, and the LED supplementary light The ratio of medium red light to blue light is 1:3, the light intensity is controlled at 3000LX, the culture temperature is controlled at 26°C, and cultured for 6 days, the density of Nannochloropsis reaches a concentration of 70 million cells / mL. Collect Nannochloropsis cell;

[0045] S2. Oil extraction: after the Nannochloropsis cells collected in step S1 are concentrated and dried, ultrasonic waves are used to break the walls of the Nannochloropsis algae, the ...

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Abstract

The invention discloses a microalgae biodiesel production method. The production method comprises the following steps of inoculating nannochloropsis oculata to a culture medium, irradiating through a red-blue dual-color LED light supplementing lamp for 8 to 14 hours per day, cultivating for 6 to 8 days at a temperature of 24 to 26 DEG C, and collecting nannochloropsis oculata cells; performing a wall breaking process on the collected nannochloropsis oculata cells through ultrasonic wave, then extracting through an organic solvent to obtain microalgae grease, and then adding the microalgae grease, methyl alcohol and a zeolite molecular sieve catalyst into to a reactor according to a mass ratio of 1 : (5 to 7) : (0.01 to 0.03) to perform esterification reaction, so as to obtain an esterification product; then separating and purifying the esterification product to obtain the biodiesel. According to the production method, the nannochloropsis oculata which is short in production period, wide in distribution, strong in photosynthesis and high in grease content is utilized as a raw material, so that the problem of vegetable oil and fat raw materials in biodiesel development is solved. The production method also has the advantages of being simple in preparation technology, high in catalytic efficiency, high in grease extraction rate, high in product yield, and the like, and also lays a good foundation for industrialization and scale of the biodiesel.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, in particular to a method for producing microalgae biodiesel. Background technique [0002] With the depletion of fossil fuels, the price of oil continues to rise sharply, and the search for renewable energy has become the need to solve the needs of human survival and maintain the sustainable development of the world economy. Biodiesel refers to oil crops such as soybeans, rapeseed, cotton, palm, etc., wild oil plants and engineering microalgae and other aquatic plant oils, animal oils, food waste oil, etc. as raw materials. A regenerative diesel fuel that replaces petrochemical diesel. The advantages of biodiesel are not limited to renewability. Compared with existing diesel, biodiesel also has the following advantages: high cetane number, which makes combustion performance better; better wetting performance, which makes fuel injection pump, engine block and connecting The wear of the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/02C11B1/10C11B3/00C11B3/12C11C3/10C12P7/64C12R1/89
CPCC11B1/025C11B1/106C11B3/00C11B3/12C11C3/10C12P7/6463Y02E50/10
Inventor 黄健峻其他发明人请求不公开姓名
Owner 南宁华侨投资区政孙贸易有限公司
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