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Antarctic krill symbiotic bacteria, Antarctic krill antioxidant peptide, preparation method and application thereof

An Antarctic krill and antioxidant peptide technology, applied in the Antarctic krill antioxidant peptide and its preparation, and the field of Antarctic krill symbiotic bacteria, can solve problems such as side effects, and achieve the effects of low cost, cost saving and mild reaction conditions

Inactive Publication Date: 2019-09-13
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Synthetic antioxidants are efficient and cheap, but due to side effects, people prefer to choose natural antioxidants

Method used

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  • Antarctic krill symbiotic bacteria, Antarctic krill antioxidant peptide, preparation method and application thereof
  • Antarctic krill symbiotic bacteria, Antarctic krill antioxidant peptide, preparation method and application thereof
  • Antarctic krill symbiotic bacteria, Antarctic krill antioxidant peptide, preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The screening of embodiment 1 bacterial strain NL-6

[0029] 1. Obtaining of fermented liquid with antioxidant activity

[0030] 1) Screening of symbiotic microbial strains Wash the Antarctic krill, grind in a mortar, take 1g of the homogenate and grind it in sterile distilled water, 150-220r / min, shake for 1-2h, take the suspension in distilled water, and gradient dilute , spread on 2216E solid medium, culture at 25-37°C for 24-48h, pick 10 strains of bacteria (number NL-(1~10)) with different appearances and culture them in 2216E liquid medium, and preserve the strains.

[0031] 2) Strain activation culture:

[0032] Inoculate 10 symbiotic strains into test tubes of 2216E liquid medium, and ferment for 8-14 hours at 25-37°C, 150-220r / min.

[0033] Inoculate the above-mentioned cultured strain into a shaker flask containing 2216E liquid medium with an inoculation amount of 4% by volume, at 25-37° C., 150-220 r / min, and ferment for 8-14 hours to obtain a seed fermenta...

Embodiment 2

[0047] Species Identification of Strain NL-6

[0048] (1) Morphological characteristics

[0049] The results of Gram staining showed that the strain NL-6 was a Gram-negative bacterium, and the colony was white or milky yellow, with smooth surface, raised and neat edges. The scanning electron microscope pictures show that the strain NL-6 is short-rod and rod-shaped, and the surface of the bacteria is smooth. Some of the newly divided bacteria are ellipsoidal. Slender, with mucous membranes between bacteria, see figure 1 . Depend on figure 1 It can be estimated that the size of a single bacterium is: width: 0.6-0.9 μm, length: 0.8-1.5 μm.

[0050] (2) Physiological and biochemical reaction characteristics

[0051] Because Gram staining showed that the bacteria were Gram-negative bacteria, the API 20E bacteria identification system could be used for physiological and biochemical analysis. The results are shown in Table 2

[0052] Table 2 Physiological and biochemical ident...

Embodiment 3

[0062] The preparation of embodiment 3 antioxidant peptides

[0063] 1) Activation of the strain

[0064] Antarctic krill symbiotic bacteria Psychrophilus NL-6 was inoculated in a test tube of 2216E liquid medium, cultivated for 10 h at 28° C., 200 r / min; In the shake flask of the fermentation medium, under the condition of 28° C., 200 r / min, the fermentation culture was carried out for 10 h. That is, the seed fermentation liquid is obtained.

[0065] 2) NL-6 fermented Antarctic krill

[0066] Antarctic krill was thawed in cold water, homogenized with a homogenizer, dissolved in distilled water at a mass volume ratio of 10% (wet weight), and sterilized under high pressure at 121° C. for 20 minutes to obtain an Antarctic krill culture medium.

[0067] The prepared seed fermentation liquid was inoculated in the Antarctic krill culture medium with an inoculation amount of 8% by volume, and fermented at 32° C. and 200 r / min for 48 hours to obtain a strain fermentation suspensio...

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Abstract

The present invention relates to an Antarctic krill symbiotic bacterium, an Antarctic krill antioxidant peptide and its preparation method and application, and belongs to the technical field of microorganisms and biologically active peptides. It was deposited in China Wuhan Type Culture Collection Center with the accession number CCTCCM 2016341. Using this strain to ferment Antarctic krill homogenate, an Antarctic krill antioxidant peptide is obtained. The active peptide component with the highest antioxidant activity is 1-5kDa component, its hydroxyl radical scavenging rate reaches 93.0%, and its reducing power is 1.32. The ABTS clearance rate was 92.4%. The polypeptide has better stability in vivo and in vitro, can better meet the needs of large-scale production, and has better development and application value as an antioxidant food additive.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and bioactive peptides, and in particular relates to an Antarctic krill symbiotic bacterium, an Antarctic krill antioxidant peptide, a preparation method and application thereof. Background technique [0002] Reactive oxygen free radicals (ROS) in the human body have certain functions, such as immune and signal transduction processes, and play an important role in cell proliferation, differentiation and apoptosis, but too much reactive oxygen free radicals will have destructive behavior. A large number of studies have proved that the mechanism of aging and aging-related diseases such as cancer, cardiovascular, autoimmune diseases, and arthritis is closely related to the excessive production of free radicals in the body or the decline in the ability to scavenge free radicals. Therefore, people have aroused strong attention to various diseases caused by free radicals causing damage and degene...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00C07K1/34A23L33/18A61K8/64A61Q19/00C12R1/01
CPCA23V2002/00A61K8/64A61K2800/10A61Q19/00C07K1/34C12P21/00C12N1/205C12R2001/01A23V2200/32A23V2250/55
Inventor 郑兰红梁方方朱美虹康道乐朱祥杰苑志欣
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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