Construction of neural stem cell derived tissue engineering spinal cord tissue

A technology for tissue engineering spinal cord and neural stem cells, applied in the field of neural stem cell-derived tissue engineering spinal cord tissue, can solve the problems of non-directional host nerve fibers, chaotic distribution of myelinating cells, and other problems

Inactive Publication Date: 2016-12-21
SUN YAT SEN UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, we are still facing some unresolved problems: that is, in the stem cell-derived neural network constructed earlier, the distribution of neurons and myelin-forming cells is random and disordered, facing the non-directional regeneration of host nerve fibers, The distribution is chaotic or intertwined, and it cannot e

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction of neural stem cell derived tissue engineering spinal cord tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] The main instrument used in the present invention, biodegradable material, experimental cells and reagents are described in detail below by specific examples:

[0011] 1. Main instruments

[0012] Ultra-clean workbench (Suzhou Purification Electronic Equipment Factory); ordinary centrifuge (Kubota Japan); constant temperature water bath box (Beijing Medical Equipment Factory); 5% CO 2 Incubator (Queue USA); Inverted Phase Contrast Microscope (Olympus Japan); Fluorescence Microscope (Leica Germany); Scanning Electron Microscope (Philips Netherlands); Transmission Electron Microscope (Philips Netherlands); Laser Confocal Imaging System (Carl Zeiss Germany); Low Temperature Oven ( Shanghai Yuejin Medical Instrument Factory); high temperature oven (Shanghai Yuejin Medical Instrument Factory); high pressure sterilizer (Jiangyin Binjiang Medical Equipment Factory); constant cold box slicer (Shandon UK); ultrapure water instrument (Molsheim France); ELISA Detector (Bio-Rad US...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a neural stem cell derived tissue engineering spinal cord tissue for simulating a spinal cord tissue structure and main cell constituents. The neural stem cell derived tissue engineering spinal cord tissue is obtained by in-vitro culture and construction through a biological tissue engineering technology and a neural stem cell induced differentiation technology, and has a white matter like structure in the peripheral part and a grey matter like structure in the central part. CNTF (ciliary neurotrophic factor) gene modified oligodendrocyte precursor cells (OPCs) are planted in the white matter like structure; and NT-3 gene and receptor thereof TrkC gene modified neural stem cells (NSCs) are planted in the grey matter like structure. When being transplanted to a spinal cord complete transection injury site, the tissue engineering spinal cord tissue has favorable cell activity and can secrete neurotrophic factors to accelerate regeneration of spinal cord injured central neuron axons; and the tissue engineering spinal cord tissue can realize corresponding functions of oligodendrocytes and neurons, and serves as a neural information transfer relay to form a synaptic connection with regenerated nerve fibers so as to repair spinal cord injured neural circuits. The tissue engineering spinal cord tissue can also be used as an in-vitro model for neural pharmacology and neural development researches.

Description

technical field [0001] The invention relates to a neural stem cell-derived tissue engineered spinal cord tissue simulating the tissue structure and main cell components of the spinal cord, which is constructed in vitro by applying biological tissue engineering technology and neural stem cell induced differentiation technology. Cells of tissue-engineered spinal cord tissue are differentiated from neural stem cells, containing white matter-like structures and gray matter-like structures; white matter-like structures are composed of neural stem cell-derived oligodendrocytes, and gray matter-like structures are composed of neural stem cell-derived neurons; available It can also be used as an in vitro model for neuropharmacology and neurodevelopmental research by transplanting it into the spinal cord injury to observe its repair effect. Background technique [0002] In the research field of spinal cord injury repair, the combined use of stem cells, neurotrophic factors and biomat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/079A61L27/38A61L27/50
CPCA61L27/383A61L27/3878A61L27/3895A61L27/50C12N5/0622A61L2430/32
Inventor 曾园山赖碧琴
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap