Construction of neural stem cell derived tissue engineering spinal cord tissue

Abstract

The invention relates to a neural stem cell derived tissue engineering spinal cord tissue for simulating a spinal cord tissue structure and main cell constituents. The neural stem cell derived tissue engineering spinal cord tissue is obtained by in-vitro culture and construction through a biological tissue engineering technology and a neural stem cell induced differentiation technology, and has a white matter like structure in the peripheral part and a grey matter like structure in the central part. CNTF (ciliary neurotrophic factor) gene modified oligodendrocyte precursor cells (OPCs) are planted in the white matter like structure; and NT-3 gene and receptor thereof TrkC gene modified neural stem cells (NSCs) are planted in the grey matter like structure. When being transplanted to a spinal cord complete transection injury site, the tissue engineering spinal cord tissue has favorable cell activity and can secrete neurotrophic factors to accelerate regeneration of spinal cord injured central neuron axons; and the tissue engineering spinal cord tissue can realize corresponding functions of oligodendrocytes and neurons, and serves as a neural information transfer relay to form a synaptic connection with regenerated nerve fibers so as to repair spinal cord injured neural circuits. The tissue engineering spinal cord tissue can also be used as an in-vitro model for neural pharmacology and neural development researches.

Description

technical field [0001] The invention relates to a neural stem cell-derived tissue engineered spinal cord tissue simulating the tissue structure and main cell components of the spinal cord, which is constructed in vitro by applying biological tissue engineering technology and neural stem cell induced differentiation technology. Cells of tissue-engineered spinal cord tissue are differentiated from neural stem cells, containing white matter-like structures and gray matter-like structures; white matter-like structures are composed of neural stem cell-derived oligodendrocytes, and gray matter-like structures are composed of neural stem cell-derived neurons; available It can also be used as an in vitro model for neuropharmacology and neurodevelopmental research by transplanting it into the spinal cord injury to observe its repair effect. Background technique [0002] In the research field of spinal cord injury repair, the combined use of stem cells, neurotrophic factors and biomat...

AI Technical Summary

Problems solved by technology

[0004] At present, we are still facing some unresolved problems: that is, in the stem cell-derived neural network constructed earlier, the distribution of neurons and myelin-forming cells is random and disordered, facing the non-directional regeneration of host nerve fibers, The distribution is chaotic or intertwined, and it cannot effectively form synaptic connections with the transplanted neurons; at the same time, it faces the problem of chaotic distribution of myelinating cells, which cannot effectively wrap the axons of neurons that need to be myelinated

Solve the difficult problem of rebuilding the own neural pathway after total spinal cord transection injury

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