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Preparation method of composite bacterial cellulose medical dressing

A technology of bacterial cellulose and composite membranes, applied in medical science, absorbent pads, bandages, etc., can solve the problem of wounds that cannot be used for dry wounds and wounds covered by hard necrotic tissue, biological dressings that cannot be used for dry wounds, inhibit In order to achieve the effect of promoting wound healing, improving mechanical properties and thermal stability, and low cost

Inactive Publication Date: 2017-01-04
仇颖莹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: Aiming at the problem that current biological dressings require a certain humidity to function, so they cannot be used for dry wounds and wounds covered by hard necrotic tissue, and have poor antibacterial and disinfection effects, provide A method for preparing a composite bacterial cellulose medical dressing. The invention first inoculates S. The seeds are inoculated into the seed medium for cultivation, and then the seed liquid is inoculated into the fermenter for anaerobic fermentation together with sodium alginate, chondroitin sulfate, etc., and then the obtained bacterial cellulose film is taken out and the bacteria and medium are removed , and finally freeze-dried and soaked in buffer solution with Burnet extract to obtain a composite bacterial cellulose medical dressing
The chondroitin sulfate in the present invention has good hygroscopicity, can absorb moisture in the air, increases its own water content, solves the problem that biological dressings cannot be used for dry wounds, and the medical dressing prepared by the present invention can penetrate hard The necrotic tissue enters the wound, so that the wound can heal quickly, and when it acts on the cartilage tissue, it can also promote the growth of cartilage tissue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019]First measure 400mL of deionized water into the beaker, heat to boiling, then stop heating, then add 20g of green tea, soak for 40min, filter, collect the filtrate, then add 30g of sucrose, 8g of peptone, 10g of yeast powder, stir for 10min, and transfer to In a sterilizing tank, sterilize at 130°C for 20 minutes to prepare a seed medium for use; then weigh 200g of mashed potatoes, 20g of glucose and 15g of agar into a petri dish, then add 1000mL of deionized water, and stir evenly. Then add 5% acetic acid solution to adjust the pH to 6.8, then transfer it into a sterilization tank, and sterilize at 120°C for 30 minutes to obtain an activated medium; then add 5g of Saccharomyces cerevisiae, 5g of Acetobacter aceti and 8g of Gluconoacetobacter xylinum was inoculated into three activation media respectively, and then placed in a constant temperature incubator, cultured at 30°C for 3 days, and the activated strains were inoculated to the spare seeds at an inoculation amount ...

example 2

[0022] First measure 300mL of deionized water into the beaker, heat to boiling, then stop heating, then add 15g of green tea, soak for 30min, filter, collect the filtrate, then add 20g of sucrose, 5g of peptone, 8g of yeast powder, stir for 5min, and transfer to In a sterilizing tank, sterilize at 120°C for 10 minutes to prepare a seed medium for use; then weigh 100g of mashed potatoes, 10g of glucose and 10g of agar into a petri dish, then add 800mL of deionized water, and stir evenly. Then add 5% acetic acid solution to adjust the pH to 6.5, then transfer it into a sterilization tank, and sterilize it at 110°C for 20 minutes to obtain an activated medium; then add 3g of Saccharomyces cerevisiae, 3g of Acetobacter aceti and 6g of Gluconoacetobacter xylinum was inoculated into three activation media respectively, and then placed in a constant temperature incubator, cultured at 20°C for 2 days, and the activated strains were inoculated to spare seeds at an inoculation amount of ...

example 3

[0025] First measure 350mL of deionized water into the beaker, heat to boiling, then stop heating, then add 18g of green tea, soak for 35min, filter, collect the filtrate, then add 25g of sucrose, 7g of peptone, 9g of yeast powder, stir for 8min, and transfer to Sterilize the tank at 125°C for 15 minutes to prepare the seed culture medium for later use; then weigh 150g of mashed potatoes, 15g of glucose and 12g of agar into a petri dish, then add 900mL of deionized water, and stir evenly. Then add 5% acetic acid solution to adjust the pH to 6.6, then transfer it into a sterilization tank, and sterilize at 115°C for 25 minutes to obtain an activated medium; then add 4g of Saccharomyces cerevisiae, 4g of Acetobacter aceti and 7g of Gluconoacetobacter xylinum was inoculated into three activated media respectively, and then placed in a constant temperature incubator, cultured at 25°C for 2.5 days, and the activated strains were inoculated into spare seeds at an inoculation amount o...

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Abstract

The invention relates to a preparation method of composite bacterial cellulose medical dressing, and belongs to the technical field of biological material preparation. According to the method, firstly, saccharomyces cerevisiae, acetobacter aceti and gluconacetobacter xylinus are inoculated into a self made activation culture medium for activation culture; then, activated strains are inoculated into a seed culture medium for culture; then, seed liquid is inoculated into a fermentation tank and is subjected to anaerobic fermentation together with sodium alginate, chondroitin-sulfuric acid and the like; next, an obtained bacterial cellulose membrane is taken out, and thalli and the culture medium are removed; finally, the bacterial cellulose membrane and sanguisorba officinalis extracts are subjected to operation such as freeze drying and buffer solution soaking; the composite bacterial cellulose medical dressing is obtained. The problem that the biological dressing cannot be used for a dry wound is solved; the medical dressing prepared by the invention can penetrate through hard necrotic tissues to enter the wound inside, so that the wound is fast healed; in addition, when the medical dressing acts on cartilage tissues, the cartilage tissue growth can also be promoted.

Description

technical field [0001] The invention relates to a preparation method of a composite bacterial cellulose medical dressing, belonging to the technical field of biological material preparation. Background technique [0002] The main function of medical dressings is to cover and protect damaged skin, and at the same time provide an environment conducive to wound healing. Traditionally, absorbent cotton gauze, gauze strips, cotton balls and cotton membranes and other natural fibrous material dressings are used to dry the wound and have physical properties. Isolation function; but there are problems such as crusting with exudate, causing wound adhesion, causing pain and new wounds when dressing is changed, as well as bacterial growth, poor moisturizing and hemostatic performance. For this reason, scholars have developed biological dressings, which can closely adhere to the wound, keep the wound moist and prevent it from drying out, and can assist locally applied drugs and endogeno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/36A61L15/40A61L15/42A61L15/28
CPCA61L15/36A61L15/28A61L15/40A61L15/42A61L2300/412C08L5/04C08L5/08
Inventor 仇颖莹孟浩影
Owner 仇颖莹
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