[0061] Example
[0062] The new generation all-element chelated food-grade liquid bacterial fertilizer of this embodiment is prepared according to the following steps:
[0063] 1) Clean the fermentation tank, check whether the tank body is leaking electricity and turn on the power to start trial operation to ensure that the waterway is unblocked and all components are operating normally.
[0064] 2) Add water and molasses to the fermentation tank, and put the bran in a stainless steel box and hang on the tank wall.
[0065] 3) Close the manhole cover, open the mixing system and heating system, pay attention to the carbon dioxide exhaust port and jacket exhaust port to keep 1/2 open.
[0066] 4) When the water temperature of the jacket reaches 90°C or the temperature in the tank reaches 85°C, turn off the stirring system and heating system, and start sealing and high temperature sterilization.
[0067] 5) After 30 minutes, start to turn on the stirring system and add water to cool down, and turn on the oxygen system for oxygenation. When the temperature drops to 60°C, add the prepared tea, sodium chloride, and carbonamide. Note: When the water level reaches 70%, close the water inlet.
[0068] 6) At this time, if the temperature in the tank is greater than 38°C, turn on the condensing device to start the cooling process; if the temperature in the tank is less than 38°C, turn on the heating tube to control the temperature at about 38°C; if the temperature in the tank is equal to 38°C, Start the next step.
[0069] 7) Determine that the temperature in the tank is 38°C, start to add the compound microbial strains, seal the fermentation system for 10 minutes, close the stirring system and the oxygen system after 10 minutes, and start the fermentation process.
[0070] 8) After 24 hours of fermentation, add the prepared complex sugar alcohol trace element chelating liquid, ammonium polyphosphate nitrogen phosphorus potassium chelating liquid and organic acid trace element chelating liquid; turn on the stirring system and stir for 20-30 minutes.
[0071] 9) Drain the liquid in the tank into a 200L plastic barrel and seal it for deep fermentation. After 15-20 days, a new generation of all-element chelated food-grade liquid bacterial fertilizer will be prepared.
[0072] The formulations of the above raw materials are shown in Table 1 and Table 2.
[0073] Among them, the complex sugar alcohol trace element chelating liquid includes sugar alcohol chelated zinc liquid, sugar alcohol chelated magnesium liquid and sugar alcohol chelated calcium liquid;
[0074] Ammonium polyphosphate nitrogen phosphorus potassium chelate liquid includes ammonium polyphosphate chelated nitrogen liquid, ammonium polyphosphate chelated phosphorus liquid and ammonium polyphosphate chelated potassium liquid;
[0075] The chelating liquid of trace elements in organic acid includes organic acid chelated iron liquid, organic acid chelated boron liquid, organic acid chelated manganese liquid, organic acid chelated potassium liquid, organic acid chelated copper liquid and organic acid chelated molybdenum liquid .
[0076] The complex sugar alcohol trace element chelating solution is prepared according to the following steps:
[0077] (1) Mix mannitol: sorbitol: xylitol: glycerol according to the mass ratio of 4:2:3:1;
[0078] (2) Raise the temperature of the mixed liquid to 70℃ first, increase the density by pressurization, and increase the pressure to 4 atmospheres. After 8-10 hours, when the density of the liquid stabilizes to 1.15g/mL, the compound can be obtained. Sugar alcohol chelating liquid;
[0079] (3) Add the trace element solution to the compound sugar alcohol mixture prepared in step 2, stir evenly, and raise the temperature to 100°C for 3 hours;
[0080] (4) Naturally cool down to 40°C, stir for 20 minutes, and stand for 24 hours after stirring to obtain a complex sugar alcohol trace element chelating solution;
[0081] The trace element solution is 0.5mol/L zinc sulfate solution, 25% 0.5mol/L magnesium sulfate solution or 30% 0.5mol/L calcium sulfate solution;
[0082] When the trace element solution is zinc sulfate solution or calcium sulfate solution, the mass fraction of the trace element solution is 30% after adding;
[0083] When the trace element solution is a magnesium sulfate solution, the mass fraction of the trace element solution after adding is 25%.
[0084] The ammonium polyphosphate chelated nitrogen solution is prepared according to the following steps:
[0085] (1) Mix ammonium phosphate and urea according to a mass ratio of 5:8, heat up to 240~260℃, keep 2~3 times atmospheric pressure for 1h to maintain polymer stability;
[0086] (2) Then maintain 2 atmospheres for 12 hours, and then slowly deflate to normal pressure to obtain ammonium polyphosphate chelated nitrogen liquid.
[0087] The ammonium polyphosphate chelated phosphorus liquid or ammonium polyphosphate chelated potassium liquid is prepared according to the following steps:
[0088] (1) Mix phosphorus pentoxide or potassium oxide and ammonium phosphate uniformly, heat up to 210°C, and maintain 2.5 times the atmospheric pressure for 10 hours;
[0089] (2) Slowly lower the temperature to 130°C and keep it for 8 hours, then lower the temperature to 80°C and keep it at 2 times the atmospheric pressure for 12 hours to obtain ammonium polyphosphate chelated phosphorus liquid or ammonium polyphosphate chelated potassium liquid;
[0090] Wherein, the mass ratio of phosphorus pentoxide to ammonium phosphate in step (1) is 5:9; the mass ratio of potassium oxide to ammonium phosphate is 4:9.
[0091] The chelating solution of trace elements in organic acid is prepared according to the following steps:
[0092] (1) Mix the carboxylic acid: medium and trace element solution with a mass ratio of 2:1, heat up to 90°C and continue to stir, and continue at 90°C for 4 to 5 hours;
[0093] (2) Cool down to 40°C and keep for 12 hours to obtain a chelating solution of trace elements in organic acids;
[0094] Among them, the trace element solution in step (1) is 0.5mol/L ferric sulfate, 0.5mol/L boric acid, 0.5mol/L manganese sulfate, 0.1mol/L potassium chloride, 0.3mol/L sulfuric acid Copper or 0.5mol/L ammonium molybdate.
[0095] Compound microbial species include Zygosaccharomyces rouxii, Rhodop seudanonas palustris, Sac-charomyces cerevisiae, Bacillus (such as Bacillus subtilis), Bacillus coagulans (Bacillus coagulans, etc.) and halophilic actinomycetes (Streptosporangium roseum, Actinopolymorphasingaporensis, Nocardiopsis halotolerans Al-Zarban et al)) .
[0096] Table 1 Amount of each raw material in the embodiment (kg)
[0097] 1 2 3 4 5 Compound microbial strains 5.5 5 4 4.5 6 Molasses 4.25 4.5 4 4.75 5 Bran 0.5 0.55 0.6 0.4 0.45 Carbonamide 0.05 0.075 0.1 0.085 0.06 Sodium chloride 0.05 0.06 0.075 0.1 0.085 tea 0.015 0.02 0.0175 0.0125 0.01 Complex sugar alcohol trace element chelating liquid 7.5 9 8.5 8 7 Ammonium polyphosphate nitrogen phosphorus potassium chelating liquid 23 22 25 20 26 Chelating liquid of trace elements in organic acid 3.5 4.5 4 5 3 water 47 45 49 46 50
[0098] Table 2 Examples of the three kinds of chelating liquid and the specific raw material ratio in the composite microorganism
[0099]
[0100]
[0101] The values in Table 2 are all proportions. In Table 2, the number of viable bacteria used in the yeast Lu's yeast is ≥5 billion/g; among the inoculum used by Rhodopseudomonas palustris, the number of live bacteria is ≥8 billion/g; in the inoculum used by brewer's yeast , The number of viable bacteria ≥ 7 billion/g; among the inoculants used for Bacillus, the number of viable bacteria is ≥ 10 billion/g; among the bacteria used for halophilic actinomycetes, the number of viable bacteria is ≥ 10 billion/g. Among them, Bacillus includes Bacillus subtilis (Bacillus subtilis), Bacillus coagulans (Bacillus coagulans), etc. Halophilic actinomycetes include Streptosporangium roseum (Streptosporangium roseum), Singapore polymorphic actinomycetes (Actinopolymorpha singaporensis), salt-tolerant Nocardiopsishalotolerans Al-Zarban et al.
[0102] Proof test 1
