Test strip for detecting estriol and applications thereof

A technology of estriol and test strips, which is applied in the field of test strips for the detection of estriol, can solve the problems of infants and adolescents' growth and development impact, susceptibility, birth defects and birth defects, and achieve short detection time and low cost. Low, long shelf life effect

Inactive Publication Date: 2017-01-11
北京安达兴业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, abuse of antibiotics and hormones often occurs in the aquaculture industry, resulting in a certain amount of estriol hormone remaining in animal-derived foods, which may cause human hormone-related diseases

Method used

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  • Test strip for detecting estriol and applications thereof
  • Test strip for detecting estriol and applications thereof
  • Test strip for detecting estriol and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 estriol detection test strip

[0041] The preparation method of this test strip mainly comprises the following steps:

[0042] 1) Prepare a conjugate release pad sprayed with estriol monoclonal antibody-colloidal gold marker;

[0043] 2) preparing a reaction membrane with a detection line coated with an estriol hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse anti-antibody;

[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the PVC bottom plate into a test strip.

[0045] The following step-by-step detailed description:

[0046] 1. Preparation of estriol hapten

[0047] Take 1.0 g of estriol and add acetonitrile to dissolve, add 0.62 g of potassium carbonate, add 0.47 g of chloroacetic acid, and stir at 60 ° C for 6 h. Detection, the basic reaction of the raw material is complete, stop the reaction...

Embodiment 2

[0080] The detection of estriol residue in the sample of embodiment 2

[0081] 1. Sample pretreatment

[0082] Take the fat-free tissue sample and homogenize it in a homogenizer for 1min (10000rpm), or cut it into pieces with scissors; weigh 2.0±0.05g of the homogenized tissue sample into a 10ml polystyrene centrifuge tube, add 1ml of the sample to extract solution, shake and shake; add 1ml of sample diluent to a polystyrene centrifuge tube, shake and shake.

[0083] 2. Test with test strips

[0084] Use a disposable straw to absorb the sample solution to be tested and drop 2-3 drops vertically into the sample hole. When the liquid flows, start timing, react for 5-10 minutes, and judge the result according to the schematic diagram. Other times are invalid.

[0085] 3. Analyze test results

[0086] Negative (-): The color of the T line is darker than that of the C line or the color is consistent, indicating that the concentration of estriol in the sample is lower than the de...

Embodiment 3

[0089] Example 3 sample detection example

[0090] 1. Detection limit test

[0091] Take blank fish, chicken, shrimp, and pork samples, add estriol to them to the final concentration of 5, 10, and 20 μg / kg, respectively, take test strips for detection, and repeat the measurement three times for each sample.

[0092] When testing fish, chicken, shrimp, and pork samples with test strips, when the concentration of estriol is 5 μg / kg, it is negative; when the concentration of estriol is 10 and 20 μg / kg, it is positive. It shows that the detection limit of this test strip for estriol in fish, chicken, shrimp and pork is 10 μg / kg.

[0093] 2. False positive rate and false negative rate test

[0094] Take 20 positive samples of fish, chicken, shrimp, and pork with known estriol content greater than 10 μg / kg (number 1-20) and 20 negative samples of fish, chicken, shrimp, and pork with content less than 10 μg / kg (No. 21-40) were tested with three batches of test strips, and the nega...

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Abstract

The invention discloses a test strip for detecting estriol and applications thereof. The test strip comprises a sample absorbing pad (1), a combined substance releasing pad (2), a reaction membrane (3), a water absorbing pad (4), and a bottom plate (7). The reaction membrane comprises a detection line (5) embedded with an estriol semi-antigen-carrier protein conjugate and a quality control line (6) embedded with a goat-anti-mouse anti-antibody. An estriol monoclonal antibody-colloid gold marker is sprayed on the combined substance releasing pad (2). The invention also provides a method using the provided estriol test strip to detect residual estriol in fish meat, chicken, shrimp meat, and pork. The provided test strip has the characteristics of simple operation, high sensitivity, fast detection speed, and low cost, and is suitable for screening of massive samples and onsite monitoring.

Description

technical field [0001] The invention relates to a test strip for detecting estriol and its application, in particular to a colloidal gold test strip for detecting estriol, which is especially suitable for use in fish, chicken, shrimp, pork and other animal-derived products. Detection of estriol residues in food. Background technique [0002] Estriol is a metabolite of estradiol in the body and is a natural estrogen mainly found in urine. However, abuse of antibiotics and hormones often exists in the aquaculture industry, resulting in a certain amount of estriol hormone remaining in animal-derived foods, which may cause human hormone-related diseases to easily occur after eating, such as breast, ovary, prostate tumors, Endocrine-related tumors, birth defects and birth defects, etc., will also have a serious impact on the growth and development of infants and adolescents. [0003] Based on the above reasons, we designed a method for the detection of estriol in animal-derived...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/577G01N33/558G01N33/52
CPCG01N33/743G01N33/523G01N33/558G01N33/577G01N2407/00
Inventor 徐宏鑫
Owner 北京安达兴业科技有限公司
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